Any of rtracklayer::import.bed('samplesubjunc.bed'), unlist(spliceGraph(TxDb)), transcripts(TxDb), exons(TxDb), or features(FDB) will produce a GRanges object containing usable features for read counting.
This function converts a suitably streamlined GRanges object into annotations which can then be used by featureCounts() to quickly count aligned reads.
The GRanges object must contain an elementMetadata column named 'id'.
The GRanges object to convert to an Rsubread annotation file
A data frame with five columns named
Tim Triche, Jr. and Wei Shi
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