Six synthetic DNA control targets are included with every nCounter Gene Expression assay.
Their concentrations range linearly (in codeset) from 128 fM
to 0.125 fM
, and they are referred to as POS_A to POS_F, respectively.
These Positive Controls are typically used to measure the efficiency of the hybridization reaction, and their step-wise concentrations also make them useful in checking the linearity performance of the assay.
Since the known concentrations of the Positive Controls increase in a linear fashion, the resulting counts should, as well.
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