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# ddPCR R package - Dean Attali 2015
# --- Results tab UI --- #
tabPanel(
title = "Results",
id = "resultsTab",
value = "resultsTab",
name = "resultsTab",
class = "fade",
icon = icon("bar-chart"),
conditionalPanel(
condition = "output.datasetChosen",
tabsetPanel(
id = "resultsTabs", type = "tabs",
# Plate data tab ----
tabPanel(
title = "Plate summary",
id = "metaTab",
value = "metaTab",
name = "metaTab",
br(),
downloadButton("saveMetaBtn", "Download plate summary"),
br(), br(),
h4(id = "aggregateDesc",
"Select multiple rows to see combined statistics",
helpPopup(paste("Selecting multiple rows by clicking on them will show a table at the",
"bottom of the page. The table will show statistics for all the selected wells combined."))
),
DT::dataTableOutput("metaTable"),
DT::dataTableOutput("metaAggregate", width = 500)
),
# Plot tab ----
tabPanel(
title = "Plot",
id = "plotTab",
br(),
div(
id = "plotOptionsSection",
tabsetPanel(
id = "plotParamsTabs", type = "pills",
tabPanel(
title = "General options",
id = "plotGeneralTab",
br(),
fixedRow(
column(
4,
selectInput(
"plotParamSubsetType", "Wells to plot",
c("Show all wells" = "all",
"Select specific wells" = "wells",
"Select specific samples" = "samples"),
selected = "all"),
conditionalPanel(
"input.plotParamSubsetType == 'wells'",
uiOutput("plotParamWellsSelect")
),
conditionalPanel(
"input.plotParamSubsetType == 'samples'",
uiOutput("plotParamSamplesSelect")
),
div(
`data-ddpcr-type` = paste(plate_types$hex_positive_pnpp, "ddpcr_plate", plate_types$fam_positive_pnpp, collapse = " "),
checkboxInput("plotParam_show_failed_wells", "Include failed wells", TRUE)
),
checkboxInput("plotParam_show_drops", "Show droplets", TRUE)
),
column(
4,
numericInput("plotParam_drops_size", "Droplets size", 1, 0, 50),
selectInput("plotParam_col_drops", "Droplets colour",
allCols, "black"),
sliderInput("plotParam_alpha_drops", "Droplets transparency",
0, 1, 0.2, 0.05, ticks = FALSE)
),
column(
4,
checkboxInput("plotParam_superimpose", "Superimpose all data in one panel", FALSE),
checkboxInput("plotParam_show_full_plate", "Show full plate", FALSE),
div(
`data-ddpcr-type` = plate_types$custom_thresholds,
checkboxInput("plotParam_show_thresholds", "Show threshold borders", TRUE),
selectInput("plotParam_col_thresholds", "Threshold borders colour",
allCols, "black")
),
div(
`data-ddpcr-type` = paste(plate_types$hex_positive_pnpp, plate_types$fam_positive_pnpp, collapse = " "),
checkboxInput("plotParam_show_mutant_freq", "set value in server/tab-results.R", TRUE),
numericInput("plotParam_text_size_mutant_freq", "set value in server/tab-results.R", 4, 0, 100)
)
)
)
),
tabPanel(
title = "Droplets",
id = "plotDropsTab",
conditionalPanel(
"!input.plotParam_show_drops",
br(),
h4("Turn on \"Show droplets\" in the General options",
"to see more droplet options."),
br()
),
conditionalPanel(
"input.plotParam_show_drops",
fixedRow(
column(
width = 3,
offset = 6,
h3(strong("Colour"))
),
column(
3,
h3(strong("Transparency"))
)
),
lapply(names(plotDropsParams), function(x) {
div(
id = sprintf("plotParamsDropRow-%s", x),
class = "plotParamsDropRow",
`data-ddpcr-type` = paste(plotDropsParams[[x]]$type, collapse = " "),
fixedRow(
column(
3,
tags$label(strong(HTML(plotDropsParams[[x]]$name)),
id = sprintf("plotParamsDropRowLabel-%s", x)),
class = "plotParamDropName"
),
column(
3,
selectInput(sprintf("plotParamDropShow-%s", x),
NULL,
c("Show" = TRUE, "Don't show" = FALSE),
plotDropsParams[[x]]$show)
),
column(
3,
selectInput(sprintf("plotParamDropCol-%s", x),
NULL, allColsDefault, plotDropsParams[[x]]$col)
),
column(
3,
sliderInput(sprintf("plotParamDropAlpha-%s", x), NULL,
0, 1, plotDropsParams[[x]]$alpha, 0.05,
ticks = FALSE)
)
)
)
})
)
),
tabPanel(
title = "Figure options",
id = "plotFigureTab",
br(),
fixedRow(
column(
4,
textInput("plotParam_title", "Title", NULL),
textInput("plotParam_xlab", "X-axis label", ""),
textInput("plotParam_ylab", "Y-axis label", "")
),
column(
4,
numericInput("plotParam_text_size_title", "Title text size", 14, 0, 100),
numericInput("plotParam_text_size_axes_labels", "X/Y labels text size", 12, 0, 100),
numericInput("plotParam_text_size_grid_labels", "Grid line labels text size", 12, 0, 100),
numericInput("plotParam_text_size_row_col", "Row/column number text size", 12, 0, 100)
),
column(
4,
selectInput("plotParam_height_type", "Height",
c("Automatic" = "auto", "Custom (specify pixels)" = "custom")),
conditionalPanel(
"input.plotParam_height_type == 'custom'",
numericInput("plotParam_height", NULL, 500, min = 0, step = 50)
),
selectInput("plotParam_width_type", "Width",
c("Automatic" = "auto", "Custom (specify pixels)" = "custom")),
conditionalPanel(
"input.plotParam_width_type == 'custom'",
numericInput("plotParam_width", NULL, 500, min = 0, step = 50)
),
checkboxInput("plotParam_show_grid", "Show grid lines", FALSE),
checkboxInput("plotParam_show_grid_labels", "Label grid lines", FALSE)
)
)
),
tabPanel(
title = "Well colours",
id = "plotWellTab",
br(),
fixedRow(
column(
6,
selectInput("plotParam_bg_unused", "Unused wells colour",
allCols, "white"),
selectInput("plotParam_bg_failed", "Failed wells colour",
allCols, "gray7"),
sliderInput("plotParam_alpha_bg_failed", "Transparency of failed wells",
0, 1, 0.7, 0.05, ticks = FALSE)
),
column(
6,
div(
`data-ddpcr-type` = paste(plate_types$hex_positive_pnpp, plate_types$fam_positive_pnpp, collapse = " "),
checkboxInput("plotParam_show_low_high_mut_freq",
"set value in server/tab-results.R",
TRUE),
selectInput("plotParam_bg_mutant", "set value in server/tab-results.R",
allCols, "purple3"),
selectInput("plotParam_bg_wildtype", "set value in server/tab-results.R",
allCols, "green3"),
sliderInput("plotParam_alpha_bg_low_high_mut_freq",
"set value in server/tab-results.R",
0, 1, 0.1, 0.05, ticks = FALSE)
)
)
)
)
)
),
actionButton(
"plotBtn",
"Plot",
class = "btn-primary"
),
hidden(downloadButton("downloadPlot", "Save figure")),
hidden(
div(id = "mainPlotContainer",
img(src = "ajax-loader.gif", id = "plotSpinner"),
plotOutput("mainPlot", width = "auto", height = "auto")
)
)
),
# Explore summary variable tab ----
tabPanel(
title = "Explore summary variable",
id = "exploreTab",
br(),
uiOutput("exploreVarOutput"),
selectInput("explorePlotType", "Graph type",
c("Box plot" = "box",
"Density plot" = "density",
"Histogram" = "histogram")),
downloadButton("saveExplorePlot", "Save figure"),
plotOutput("explorePlot", width = "500")
),
# Droplets data tab ----
tabPanel(
title = "Droplets data",
id = "dropletsTab",
br(),
downloadButton("saveDropletsBtn", "Download droplets data"),
br(), br(),
DT::dataTableOutput("dropletsTable", width = 500),
div(id = "clustersMappingOuter",
span(id = "clustersMappingInner",
div(id = "clustersTitle", "Clusters"),
uiOutput("clustersMapping")
)
)
)
)
)
)
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