# suppressMessages(library(digitalDLSorteR)) knitr::opts_chunk$set( echo = TRUE, dpi = 80, fig.width = 8, fig.height = 4.5, fig.align = "center", eval = digitalDLSorteR:::.checkPythonDependencies(alert = "none") )
digitalDLSorteR implements all the necessary tools to build new context-specific deconvolution models from previously characterized scRNA-Seq data. Taking into account the variability of some cell types depending on the context in which they are found, we aim to generate different models to each environment. From our view, this opens a door to more accurate and specific models instead of using generic transcriptional profiles as a reference (i.e., the use of peripheral blood mononuclear cell (PBMC) to estimate the proportions of tumor-infiltrating lymphocytes in oncological settings). In this vignette, we will show the workflow required to build new context-specific deconvolution models. For this, simulated data are going to be used in order to avoid long runtimes. The performance of a real model can be explored in the article Performance of a real model: deconvolution of colorectal cancer samples.
This workflow is computationally more expensive than using pre-trained models, so we recommend building new models if you want to deconvolute samples from an unavailable model or in case you think your scRNA-Seq data provide a better picture of this environment than the ones already offered in digitalDLSorteRmodels
. In any case, digitalDLSorteR provides a set of functionalities that make this process easier and computationally cheaper in terms of RAM usage: batch processing of data and the use of the HDF5Array and DelayedArray packages (see the article HDF5 files as back-end for more information). Furthermore, all steps are centralized in the DigitalDLSorter
S4-class, the core of digitalDLSorteR, to provide a good user experience and keep all the information tidy in the same object.
The main steps needed to build new models are summarized below, but you can find a visual summary in the following figure.
DigitalDLSorter
objectDigitalDLSorter
object and trained modelsknitr::include_graphics("workflow_building_models.png")
DigitalDLSorter
object {#load}First, we have to load scRNA-Seq data into a DigitalDLSorter
object. This S4 class contains all the slots needed to store the data generated during the construction of new deconvolution models. The information to be provided consists of three elements:
This information may come from a pre-loaded SingleCellExperiment
object or from files stored on disk. For the latter, tsv, tsv.gz, sparse matrices (mtx) and HDF5 (h5) formats are accepted. Finally, data will be stored as a SingleCellExperiment
object in the single.cell.real
slot of the new DigitalDLSorter
object.
To do so, we first simulate a scRNA-Seq data matrix of 500 rows (genes) and 100 columns (samples) by randomly sampling from a Poisson distribution. Note: these simulated data are only for descriptive purposes to show functionalities of the package, it is not intended to generate realistic transcriptomic data.
## loading the packages suppressMessages(library(digitalDLSorteR)) suppressMessages(library(SingleCellExperiment)) ## set seed for reproducibility set.seed(123) sce <- SingleCellExperiment( matrix( stats::rpois(50000, lambda = 5), nrow = 500, ncol = 100, dimnames = list(paste0("Gene", seq(500)), paste0("RHC", seq(100))) ), colData = data.frame( Cell_ID = paste0("RHC", seq(100)), Cell_Type = sample( x = paste0("CellType", seq(5)), size = 100, replace = TRUE ) ), rowData = data.frame( Gene_ID = paste0("Gene", seq(500)) ) )
Then, we provide this SingleCellExperiment
object to loadSCProfiles
as follows:
DDLSToy <- loadSCProfiles( single.cell = sce, cell.ID.column = "Cell_ID", gene.ID.column = "Gene_ID", min.cells = 0, min.counts = 0, project = "ToyExample" ) DDLSToy
In this case, we are loading single-cell profiles from a SingleCellExperiment
object, but it could be done by directly providing files as follows:
## this code will not be run toyFiles <- c("countsMatrix.tsv.gz", "cellsMetadata.tsv.gz", "genesMetadata.tsv.gz") DDLSToy <- loadSCProfiles( single.cell = toyFiles, cell.ID.column = "Cell_ID", gene.ID.column = "external_gene_name", min.cells = 0, min.counts = 0, project = "ToyExampleBreast" )
In documentation, you can see all the parameters that loadSCProfiles
offers to preprocess the loaded data, such as min.counts
and min.cells
, fun.aggregate
, etc. In addition, in case of working with very large scRNA-Seq datasets, digitalDLSorteR allows to use HDF5 files as back-end to handle data that do not fit in RAM using the HDF5Array and DelayedArray packages. We only recommend their use in actual cases of huge datasets. HDF5 files, despite being very powerful and useful for dealing with RAM problems, make processes much slower. As an example, the following code chunk would create an HDF5 file with the scRNA-seq data that allows working without loading them into RAM. See the documentation for more details.
DDLSToy <- loadSCProfiles( single.cell = toyFiles, cell.ID.column = "Cell_ID", gene.ID.column = "external_gene_name", min.cells = 0, min.counts = 0, file.backend = "singlecell_data.h5", project = "ToyExampleBreast" )
digitalDLSorteR offers the possibility to simulate new single-cell profiles from real ones to increase signal and variability in small datasets or when under-represented cell types are present. This step is optional but recommended in these situations. The estimateZinbwaveParams
and simSCProfiles
functions are used for this purpose.
First step is to estimate a set of parameters that fit the real single-cell data to simulate new realistic single-cell profiles. We chose the ZINB-WaVE framework [@Risso2018] that estimates the parameters of a ZINB (zero-inflated negative binomial) distribution. It was chosen for its ability to accommodate not only variability within a particular cell type, but also variability within the entire experiment.
This process is performed by the estimateZinbwaveParams
function, which makes use of the zinbwave package. You must specify the column corresponding to cell types in the cells metadata, and other cell/gene covariates can be added based on your experimental design, such as patient, gender or gene length. This process may take a few minutes to run, so be patient. In any case, you can adjust the number of used threads in some steps during the estimation with the threads
argument depending on your computational resources by the BiocParallel package. In case of large datasets with some cell types under-represented, the subset.cells
parameter allows making a subset of cells to speed up the process. With the following code, a total of 40 cells will be taken from the original scRNA-Seq data and used to fit a ZINB-WaVE model.
DDLSToy <- estimateZinbwaveParams( object = DDLSToy, cell.ID.column = "Cell_ID", gene.ID.column = "Gene_ID", cell.type.column = "Cell_Type", subset.cells = 40, threads = 1, verbose = TRUE )
DDLSToy
Once ZINB-WaVE parameters have been estimated, the simSCProfiles
function uses them to simulate new single-cell profiles based on the real ones. It is done by randomly sampling from a negative binomial distribution with the estimated ZINB parameters $\mu$ and $\theta$, and introducing dropouts by sampling from a binomial distribution with the estimated probability $\pi$. You must specify the number of cell profiles per cell type to be generated (n.cells
). For example, if your data set is composed of 5 cell types and n.cells
is equal to 10, the number of simulated profiles will be 50.
DDLSToy <- simSCProfiles( object = DDLSToy, cell.ID.column = "Cell_ID", cell.type.column = "Cell_Type", n.cells = 10, suffix.names = "_Simul", verbose = TRUE )
These simulated single-cell profiles are stored in single.cell.simul
slot to be used to simulate new bulk RNA-Seq profiles with a known cell composition.
DDLSToy
In this step, it is also possible to store the new simulated single-cell profiles in a HDF5 file. Indeed, they can be simulated in batches, avoiding loading all data into RAM. The code would be as follows:
DDLSToy <- simSCProfiles( object = DDLSToy, cell.ID.column = "Cell_ID", cell.type.column = "Cell_Type", n.cells = 10, suffix.names = "_Simul", file.backend = "simulated_singlecell_data.h5", block.processing = TRUE, block.size = 20, # number of single-cell profiles simulated per batch verbose = TRUE )
To simulate pseudo-bulk samples with a known cell composition, it is necessary to generate a cell composition matrix that determines the proportion of every cell type in every sample. This is carried out using the generateBulkCellMatrix
function that stores these results in the prob.cell.types
slot as a ProbMatrixCellTypes
object.
This process starts with dividing single-cell profiles into training and test data (see train.freq.cells
argument in documentation). Each subset will be used to generate each subset of bulk samples (training and test) in order to avoid any distortion of results during model evaluation. Then, proportions are generated using six different methods to avoid biases during training due to the cellular composition of the simulated bulk RNA-Seq samples:
prob.design
argument). This information can be inferred from the single cell analysis itself or from the literature.Proportion of each type of sample can be set by the proportion.train
and proportion.test
arguments. Moreover, prob.zero
controls the number of zeros (number of cell types that will be zero in each sample) produced by each method. This parameter was introduced because otherwise cell compositions that are strongly biased towards a certain cell type. To account for this, prob.zero
generate sparse compositions depending on the probability entered for each method. Finally, other important parameters are n.cells
, which determines the number of cells that will compose each pseudo-bulk sample, and num.bulk.samples
, which defines the total number of pseudo-bulk samples generated (training + test subsets). The code would be as follows:
## for reproducibility set.seed(123) ## prior knowledge for prob.design argument probMatrix <- data.frame( Cell_Type = paste0("CellType", seq(5)), from = c(rep(1, 2), 1, rep(30, 2)), to = c(rep(15, 2), 50, rep(70, 2)) ) DDLSToy <- generateBulkCellMatrix( object = DDLSToy, cell.ID.column = "Cell_ID", cell.type.column = "Cell_Type", prob.design = probMatrix, num.bulk.samples = 250, n.cells = 100, verbose = TRUE )
DDLSToy
Remember that this is a simulated example. In real circumstances, depending on the number of single-cell profiles loaded/simulated at the beginning and computational resources, about 20,000-30,000 samples would be recommended.
You can inspect the cell composition matrix created in this step with the getter function getProbMatrix
:
head(getProbMatrix(DDLSToy, type.data = "train")) tail(getProbMatrix(DDLSToy, type.data = "train"))
Moreover, distributions can be plotted using the showProbPlot
function:
lapply( 1:6, function(x) { showProbPlot( DDLSToy, type.data = "train", set = x, type.plot = "boxplot" ) } )
Now, simulated cell proportions are used to create pseudo-bulk samples. They are simulated by aggregating single-cell profiles of each cell type according to these proportions. The idea is to simulate a real bulk RNA-Seq data in which the gene expression levels of each cell are aggregated into a single sample. Therefore, this expression matrix will be generated according to the following equation:
\begin{equation} T_{ij} = \sum_{k = 1}^{K} \sum_{z = 1}^Z C_{izk} \end{equation}
\begin{equation}
\textrm{such as} \left{
\begin{array}{l}
i = 1 \ldots M;\
j = 1 \ldots N \
Z = 1 \ldots \textrm{n.cells} \cdot P_{kj} \
\sum_{k = 1}^K Z \cdot P_{kj} = \textrm{n.cells}
\end{array}
\right.
\end{equation}
where $T_{ij}$ is the expression level of gene $i$ in bulk sample $j$; $C_{izk}$ is the expression level of gene $i$ in cell $z$ in bulk sample $j$; and $P_{kj}$ is the proportion of cell type $k$ in bulk sample $j$ (the cell composition matrix generated in the previous step). $Z$ represents the number of cells that will make up the proportion of cell type $k$ in the bulk sample $j$ and corresponds to the n.cells
parameter from the generateBulkCellMatrix
function. Cells are randomly sampled based on their cell type and how they were divided into training and test subsets. This step is performed by simBulkProfiles
as follows:
DDLSToy <- simBulkProfiles( object = DDLSToy, type.data = "both", pseudobulk.function = "MeanCPM" )
These samples are stored as a SummarizedExperiment
object in the bulk.simul
slot where they can be inspected at any time:
DDLSToy
Again, these pseudo-bulk samples can be stored as an HDF5 file. This is the most recommended step of digitalDLSorteR to use this functionality, as it is the most computationally expensive part of the package and these samples will only be accessed during training and evaluation of Deep Neural Network (DNN) model. As in simSCProfiles
, samples can be simulated in batches and a desired number of threads can also be set:
DDLSToy <- simBulkProfiles( object = DDLSToy, type.data = "both", file.backend = "pseudobulk_samples.h5", block.processing = TRUE, block.size = 1000, threads = 2 )
digitalDLSorteR (>= 0.2.0) provides three methods to geneate pseudo-bulk samples through the pseudobulk.function
argument:
"MeanCPM"
: single-cell profiles (raw counts) are transformed into CPMs and cross-cell averages are calculated. Then, $log_2(CPM + 1)$ are calculated. In this way, every cell contributes the same weight rather than creating biases due to sequencing depth or the fact that some cell types express more than others."AddCPM"
: single-cell profiles (raw counts) are transformed into CPMs and are added up across cells. Then, $log_2(CPM + 1)$ are calculated. This method is practically identical to the previous one."AddRawCount"
: single-cell profiles (raw counts) are added up across cells. Then, $log_2(CPM + 1)$ are calculated. Which method is chosen depends on the technology used to generate the starting single-cell RNA-seq data. In future releases, we will make available new examples for each situation.
Once pseudo-bulk samples have been generated, DNN model can be trained and evaluated. trainDigitalDLSorterModel
is the function in charge of both steps and uses the keras package with tensorflow as back-end. If you want more information about keras or have any problems during its installation, please see Keras/TensorFlow installation and configuration for more details. In any case, the installTFpython
function automates this process, so we recommend its use.
In terms of architecture and model parameters, trainDigitalDLSorterModel
implements by default two hidden layers with 200 neurons each, although any of these parameters can be modified through the trainDigitalDLSorterModel
parameters. In addition, for a more customized model, it is possible to provide a pre-built model in the custom.model
parameter. See the documentation for more details.
The code with default parameters is as follows:
DDLSToy <- trainDigitalDLSorterModel(object = DDLSToy, scaling = "standarize")
In the end, DDLSToy
will contain a DigitalDLSorterDNN
object with all the information associated with the model in the trained.model
slot: a keras.engine.sequential.Sequential
object with the trained model, metrics and loss function histories during training, and prediction results on test data.
DDLSToy
Since this is a 'toy' example, results are not very accurate. For a real example of a well trained model, see the Performance of a real model: deconvolution of colorectal cancer samples vignette.
on.the.fly
argumentThe on.the.fly
argument of trainDigitalDLSorterModel
allows generating pseudo-bulk samples 'on the fly'. It means it is possible to skip the simulation of pseudo-bulk samples performed by the simBulkProfiles
function, and create samples at the same time as DNN model is being trained. Of course, runtimes during training may increase, but data is not loaded into RAM or stored in large HDF5 files. To use this functionality, it is only necessary to set on.the.fly = TRUE
as follows:
DDLSToy <- trainDigitalDLSorterModel(object = DDLSToy, on.the.fly = TRUE)
While metrics from prediction results on test data are informative about the performance of the model, a more comprehensive analysis is needed. For this task, digitalDLSorteR provides a set of visualization functions to represent a variety of error metrics in different ways.
First, calculateEvalMetrics
is needed to calculate the error metrics to be plotted. By default, absolute error (AbsErr
), proportional absolute error (ppAbsErr
), squared error (SqrErr
) and proportional squared error (ppSqrErr
) are calculated for every sample of test data. Furthermore, they are all aggregated using their average values according to three criteria: each cell type (CellType
), proportion bins of 0.1 (pBin
) and number of different cell types (nCellTypes
).
DDLSToy <- calculateEvalMetrics(object = DDLSToy)
Now, these results can be plotted by the following battery of functions.
distErrorPlot
and barErrorPlot
: error distributions {#dist-err}The distErrorPlot
function allows pltting how errors are distributed in different ways. Moreover, it allows to split the plots in different panels representing how errors are distributed by a given variable. Available variables are cell types (CellType
) and number of cell types in samples (nCellTypes
). In the following example, we will represent the overall errors by cell type.
distErrorPlot( DDLSToy, error = "AbsErr", x.by = "CellType", color.by = "CellType", error.labels = FALSE, type = "boxplot", size.point = 1 )
Now, if you want to know if there is a bias towards a specific cell type, yo can use facet.by
parameter to split plots by cell type:
distErrorPlot( DDLSToy, error = "AbsErr", facet.by = "CellType", color.by = "nCellTypes", type = "violinplot", size.point = 1 )
It is also possible to represent errors by number of different cell types in samples:
distErrorPlot( DDLSToy, error = "AbsErr", color.by = "CellType", facet.by = "nCellTypes", type = "boxplot", size.point = 1 )
Finally, with barErrorPlot
, the mean error values with their corresponding dispersion ranges can be plotted as follows:
barErrorPlot(DDLSToy, error = "MAE", by = "CellType")
corrExpPredPlot
: correlation plots between predicted and expected proportions {#corr-err}Ideally, the model should provide predictions that linearly match the actual proportions. Therefore, you can generate correlation plots to assess the performance. By default, the Pearson's coefficient correlation ($R$) and the concordance correlation coefficient ($CCC$) are shown as annotations on the plots. The latter is a more realistic measure as it decreases as the points move away from the identity.
corrExpPredPlot( DDLSToy, color.by = "CellType", size.point = 1, corr = "both" )
As in the previous case, plots can be split according to different variables. Now, let's split the results by CellType
and nCellTypes
as an example:
corrExpPredPlot( DDLSToy, color.by = "CellType", facet.by = "CellType", size.point = 1, filter.sc = FALSE, corr = "both" )
corrExpPredPlot( DDLSToy, color.by = "CellType", facet.by = "nCellTypes", size.point = 1, corr = "both" )
blandAltmanLehPlot
: Bland-Altman agreement plots {#bland-err}The blandAltmanLehPlot
function allows to display Bland-Altman agreement plots. This is a kind of graphical method for comparing the level of agreement between two different sets of values. The differences between predictions and actual proportions are plotted against their averages. The central dashed line represents the mean difference, while the two red dashed lines are the limits of agreement, which are defined as the mean difference plus and minus 1.96 times the standard deviation of the differences. 95% of the differences are expected to fall between these two limits, so the wider the margins, the worse the performance. It is also possible to display it in $log_2$ space.
blandAltmanLehPlot( DDLSToy, color.by = "CellType", log.2 = FALSE, size.point = 1, filter.sc = TRUE, density = TRUE, )
In addition, this function has the same behaviour as previous ones, as it is possible to split plots:
blandAltmanLehPlot( DDLSToy, color.by = "nCellTypes", facet.by = "nCellTypes", log.2 = FALSE, size.point = 1, filter.sc = TRUE, density = FALSE )
Once the model has been evaluated, new bulk RNA-seq data can be loaded into the DigitalDLSorter
object to be deconvoluted. Here, we are going to simulate bulk RNA-Seq data from a Poisson distribution:
countsBulk <- matrix( stats::rpois(100, lambda = sample(seq(4, 10), size = 100, replace = TRUE)), nrow = 40, ncol = 15, dimnames = list(paste0("Gene", seq(40)), paste0("Bulk", seq(15))) )
And load them into the object:
suppressMessages(library(SummarizedExperiment, quietly = TRUE)) seExample <- SummarizedExperiment(assay = list(counts = countsBulk)) DDLSToy <- loadDeconvData( object = DDLSToy, data = seExample, name.data = "Simulated.example" )
Then, with the deconvDigitalDLSorterObj
function, these new samples can be deconvoluted into the cell types considered by the model and predicted proportions can be represented by the barPlotCellTypes
function. The cell composition matrix is stored in the deconv.results
slot.
DDLSToy <- deconvDigitalDLSorterObj( object = DDLSToy, name.data = "Simulated.example", normalize = TRUE, scaling = "standarize", verbose = FALSE ) ## plot results barPlotCellTypes( DDLSToy, name.data = "Simulated.example", rm.x.text = TRUE, color.line = "black" )
DigitalDLSorter
object and trained models {#save}digitalDLSorteR provides different ways to save models on disk and to retrieve them in the DigitalDLSorter
object. First, you can save DigitalDLSorter
objects as RDS files. Since this file format only accepts native R objects, they are not able to store complex data structures such as keras Python objects (keras.engine.sequential.Sequential
class). To make it possible, digitalDLSorteR implements a saveRDS
generic function that converts the keras model object into a list with network architecture and weights after training. These two pieces of information are the minimal part needed to perform new predictions. When the model is to be used, it is compiled back to a keras.engine.sequential.Sequential
object.
## this code will not be run saveRDS(object = DDLSToy, file = "valid/path")
However, the optimizer state is not saved in this way. To offer the possibility to save the complete model, digitalDLSorteR has the saveTrainedModelAsH5
function to save on disk the DNN model, and loadTrainedModelFromH5
to load-back models into DigitalDLSorter
objects. Note that in this way just the keras model is saved as an HDF5 file.
## this code will not be run saveTrainedModelAsH5(DDLSToy, file.path = "valid/path") DDLSToy <- loadTrainedModelFromH5(DDLSToy)
Any scripts or data that you put into this service are public.
Add the following code to your website.
For more information on customizing the embed code, read Embedding Snippets.