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#' mdftracks: Read and Write MTrackJ Data Files
#'
#' Reads and writes MTrackJ Data Files (`.mdf`).
#' Supports clusters, 2D data, and channel information. If desired, generates
#' unique track identifiers based on cluster and id data from the `.mdf` file.
#'
#' @docType package
#' @name mdftracks
#' @seealso [MTrackJ Data Format](https://imagescience.org/meijering/software/mtrackj/format/)
NULL
# Store package local variables
pkg.env <- new.env(parent = emptyenv())
pkg.env$mtrackj.version <- '1.5.1'
pkg.env$mtrackj.header <- "MTrackJ %s Data File"
#' Read an MTrackJ Data File (`.mdf`)
#'
#' Reads an MTrackJ Data File (`.mdf`) file in a data.frame.
#'
#' @param file MTrackJ Data File (`.mdf`) file with tracking data.
#' @param drop.Z drop z-coordinate (for 2D data)
#' @param include.point.numbers include the point numbers in the mdf file
#' (**NB** these can be different from the time/frame points)
#' @param include.channel include channel information
#' @param generate.unique.ids combine cluster and id columns to get unique ids
#' @param text character string: if file is not supplied and this is, then data
#' are read from the value of text via a text connection. Notice that a literal
#' string can be used to include (small) data sets within R code.
#' @param fileEncoding character string: if non-empty declares the encoding to
#' be used on a file (not a connection) so the character data can be re-encoded
#' as they are written. See [base::file()].
#'
#' @family mdftracks functions
#'
#' @export
#'
#' @seealso [MTrackJ Data Format](https://imagescience.org/meijering/software/mtrackj/format/)
#'
#' @examples
#' read.mdf(system.file("extdata", "example.mdf", package = 'mdftracks'))
#'
read.mdf <- function(file, drop.Z = F, include.point.numbers = FALSE,
include.channel = F, generate.unique.ids = F, text,
fileEncoding = "") {
if (missing(file) && !missing(text)) {
file <- textConnection(text, encoding = "UTF-8")
on.exit(close(file))
}
if (is.character(file)) {
file <- if (nzchar(fileEncoding))
file(file, "rt", encoding = fileEncoding)
else file(file, "rt")
on.exit(close(file))
}
if (!inherits(file, "connection"))
stop("'file' must be a character string or connection")
if (!isOpen(file, "rt")) {
open(file, "rt")
on.exit(close(file))
}
# Read first line
mdf.lines <- readLines(file, n = 1)
if(!grepl(sprintf(pkg.env$mtrackj.header, '[0-9]+(.[0-9]+)*'), mdf.lines)) {
stop("does not appear to be an MTrackJ Data File")
}
# message(mdf.lines) # Print mdf version info from file
mdf.lines <- c(mdf.lines, readLines(file))
cluster.bounds <- getClusterBounds(mdf.lines)
cluster.lines.list <- getClusterLines(mdf.lines, cluster.bounds)
cluster.track.list <- lapply(cluster.lines.list, getClusterTracks)
# Add cluster number
cluster.track.list <- mapply(function(df, id) {
df$cluster <- id
df
}, cluster.track.list, cluster.bounds$id, SIMPLIFY = F)
# Merge to one data frame
df <- do.call(rbind, cluster.track.list)
# Select columns of interest
cols <- c('cluster', 'id', 'time', 'x', 'y')
if(!drop.Z) { cols <- c(cols, "z")}
if(include.point.numbers) { cols <- c(cols, "point")}
if(include.channel) { cols <- c(cols, "channel")}
df <- df[, cols]
# Generate unique ids
if(generate.unique.ids) {
if(length(unique(df$cluster)) == 1) {
# Only one cluster, uid = id
df$uid <- df$id
} else {
# Multiple clusters, create uid based on cl and id
df$uid <- as.numeric(factor(paste(df$cluster, df$id, sep = ".")))
}
}
attr(df, "doc") <- paste0("Read from ", mdf.lines[[1]])
df
}
#' Write an MTrackJ Data File (`.mdf`)
#'
#' Writes a data.frame with tracking information as an MTrackJ Data File (`.mdf`)
#' file. Allows flexible column specification, and to avoid errors the column
#' mapping used for writing is reported back to the user. Writing tracking data in
#' 'id time x y z' format, for example, from the celltrackR package, doesn't
#' require additional arguments.
#'
#' @family mdftracks functions
#'
#' @export
#'
#' @param x the data.frame with track information.
#' @param file either a character string naming a file or a connection open for
#' writing. "" indicates output to the console.
#' @param cluster.column index or name of the column that contains the cluster
#' ID.
#' @param id.column index or name of the column that contains the track
#' ID (either the id in the cluster or a unique id).
#' @param time.column index or name of the column that contains elapsed time
#' @param scale.time a value by which to multiply each time point. Useful for
#' changing units, or specifying the time between positions if the time is given
#' in frames.
#' @param pos.columns vector containing indices or names of the columns that
#' contain the spatial coordinates. If this vector has two entries, the data is
#' assumed to be 2D and the z coordinate is set to 1.0.
#' @param channel.column index or name of the column that contains channel
#' information. If there is no channel column `default.channel` will be used.
#' @param default.channel channel to be used if `channel.column` is not
#' specified.
#' @param point.column index or name of the column that contains point ID. If
#' there is no point column, points will be numbered automatically (**NB** points
#' are not necessarily the same as frames).
#' @param fileEncoding character string: if non-empty declares the encoding to
#' be used on a file (not a connection) so the character data can be re-encoded
#' as they are written. See [base::file()].
#'
#' @seealso [MTrackJ Data Format](https://imagescience.org/meijering/software/mtrackj/format/)
#' @seealso [celltrackR](https://github.com/ingewortel/celltrackR)
#'
#' @examples
#' \dontrun{
#' # Output to file
#' write.mdf(mdftracks.example.data, '~/example.mdf', id.column = 'uid',
#' time.column = 't', pos.columns = letters[24:26])
#' }
#'
#' # Output to stdout with cluster column
#' write.mdf(mdftracks.example.data, cluster.column = 'cl',
#' id.column = 'id', time.column = 't', pos.columns = letters[24:26])
#'
#' # Output to stdout using data in (id, t, x, y, z) format
#' write.mdf(mdftracks.example.data[, c('uid', 't', letters[24:26])])
#'
#' @importFrom utils capture.output
write.mdf <- function(x, file = "", cluster.column = NA, id.column = 1,
time.column = 2, scale.time = 1, pos.columns = c(3,4,5),
channel.column = NA, point.column = NA,
default.channel = 1, fileEncoding = "") {
cn <- getColumnNames(colnames(x), cluster.column, id.column,
time.column, pos.columns, channel.column,
point.column)
message.cn <- cn
names(message.cn) <- c("cluster", "id", "time", letters[24:26], "channel",
"point")
message(paste(c("Using the following column mapping:",
capture.output(message.cn)), collapse = '\n'))
# Get rid of factor columns
factor.columns <- sapply(x[ , cn[!is.na(cn)]], is.factor)
if(any(factor.columns)) {
# Get factor.column names
fcn <- names(factor.columns[factor.columns == T])
message(paste("Converting factor to numeric in columns:",
paste(fcn, collapse = ", ")))
x[fcn] <- lapply(x[fcn], function(x) as.numeric(as.character(x)))
}
if(is.na(cn['cl'])) {
cn['cl'] <- "cl"
x[cn['cl']] <- 1
}
if(is.na(cn['ch'])) {
cn['ch'] <- "ch"
x[cn['ch']] <- default.channel
}
if(is.na(cn['z'])) {
cn['z'] <- "z"
x[cn['z']] <- 1
}
generate.points <- F
if(is.na(cn['p'])) {
generate.points <- T
cn['p'] <- 'p'
}
# Connection stuff, stolen from write.table
if (file == "")
file <- stdout()
else if (is.character(file)) {
file <- if (nzchar(fileEncoding))
file(file, "w", encoding = fileEncoding)
else file(file, "w")
on.exit(close(file))
}
if (!inherits(file, "connection"))
stop("'file' must be a character string or connection")
if (!isOpen(file, "w")) {
open(file, "w")
on.exit(close(file))
}
writeLines(sprintf(pkg.env$mtrackj.header, pkg.env$mtrackj.version), file,
sep = '\n')
writeLines("Assembly 1", file, sep = '\n')
cluster.l <- split(x, x[cn['cl']])
for(cluster in names(cluster.l)) {
writeLines(sprintf("Cluster %d", as.numeric(cluster)), file, sep = '\n')
track.l <- split(cluster.l[[cluster]], cluster.l[[cluster]][cn['id']])
for(track in names(track.l)) {
writeLines(sprintf("Track %d", as.numeric(track)), file, sep = '\n')
track.data <- track.l[[track]]
track.data <- track.data[sort.list(track.data[[cn['t']]]), ]
if(generate.points) {
track.data[cn['p']] <- 1:nrow(track.data)
}
point.l <- split(track.data, track.data[cn['p']])
for(point in names(point.l)) {
pd <- point.l[[point]]
writeLines(paste("Point", as.integer(point), pd[cn['x']], pd[cn['y']],
pd[cn['z']], pd[cn['t']] * scale.time, pd[cn['ch']]),
file, sep = '\n')
}
}
}
writeLines("End of MTrackJ Data File", file, sep = '\n')
}
# getMTrackJVersion <- function(mdf.lines) {
# first.line.split <- strsplit(mdf.lines[[1]], " ")[[1]]
# stopifnot(first.line.split[1] == "MTrackJ")
# first.line.split[2]
# }
getTrackBounds <- function(mdf.lines) {
track.lines <- grep("^Track", mdf.lines)
track.nrs <- as.numeric(sapply(strsplit(mdf.lines[track.lines], " "), "[[", 2))
data.frame(id = track.nrs, begin = track.lines + 1, end = c(track.lines[-1], length(mdf.lines)))
}
getClusterBounds <- function(mdf.lines) {
cluster.lines <- grep("^Cluster", mdf.lines)
cluster.nrs <- as.numeric(sapply(strsplit(mdf.lines[cluster.lines], " "), "[[", 2))
# -1 for the last line in the file
data.frame(id = cluster.nrs, begin = cluster.lines + 1, end = c(cluster.lines[-1], length(mdf.lines)) - 1)
}
getClusterLines <- function(mdf.lines, cluster.bounds) {
cluster.bounds.l <- split(cluster.bounds, cluster.bounds$id)
cluster.lines.l <- invisible(lapply(cluster.bounds.l, function(x) {
mdf.lines[x$begin:x$end]
}))
}
#' @importFrom utils read.delim
getClusterTracks <- function(cluster.lines) {
track.bounds <- getTrackBounds(cluster.lines)
track.bounds.l <- split(track.bounds, track.bounds$id)
track.df.l <- invisible(lapply(track.bounds.l, function(x) {
# Get correct rows from cluster.lines
t <- cluster.lines[x$begin:x$end]
# Filter out Point lines
t <- t[grep("^Point", t)]
# Read lines as data frame
t.df <- read.delim(sep = " ", header = F, text = t)
# Rename columns
colnames(t.df) <- c('id', 'point', 'x', 'y', 'z', 'time', 'channel')
# Put in the correct track id
t.df$id <- x$id
t.df
}))
# Bind list together to get the DF, then convert to data matrix (make numeric),
# then back to DF
as.data.frame(data.matrix(do.call(rbind, track.df.l)), stringsAsFactors = F)
}
getColumnNames <- function(col.names, cluster.column = NA, id.column = 1,
time.column = 2, pos.columns = c(3,4,5),
channel.column = NA, point.column = NA) {
if(length(pos.columns) < 3) {
pos.columns <- c(pos.columns, rep(NA, 3 - length(pos.columns)))
}
col.args <- as.character(c(cluster.column, id.column, time.column,
pos.columns, channel.column, point.column))
# Try to match the provided column names to the column names
matched.col.names <- col.names[match(col.args, col.names)]
# Try to match the provided column indices to the column indices
matched.col.indices <- match(col.args, seq_len(length(col.names)))
# Merge the 2 results: get names for the columns specified as index
non.matched.col.names <- is.na(matched.col.names)
matched.col.names[non.matched.col.names] <-
col.names[matched.col.indices[non.matched.col.names]]
# We only care about the columns that are not NA by default, however, if a
# column is specified (not NA in col.args) it should match an existing column
col.specified.not.matched <- !is.na(col.args) & is.na(matched.col.names)
if (any(col.specified.not.matched)) {
stop("Column(s) not found: ", paste(col.args[col.specified.not.matched],
collapse = ","))
}
colnames.names <- c("cl", "id", "t", "x", "y", "z", "ch", "p")
names(matched.col.names) <- colnames.names
matched.col.names
}
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