GSE20986_design: Comparative Gene Expression Profiling of HUVEC and Ocular...

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Evolution combined with genomic study elucidates genetic bases of isobutanol tolerance in Escherichia coli

Platform: GPL570 [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array
Organism: Homo sapiens
Experiment type: Expression profiling by array

To compare the gene expression profiles of unpassaged, proliferating HUVEC and human iris, retinal and choroidal microvascular endothelial cells. Gene expression profiling revealed significant differences between HUVEC and ocular microvascular endothelial cells suggesting that HUVE cells may not be a suitable surrogate when studying pathophysiological mechanisms of ocular disorders. There were significant differences in the gene expression of important cell signalling pathways in human retinal and choroidal ECs. These differences may be important in the mechanisms and treatment of choroidal and retinal neovascularisation.

Overall design
12 arrays are included. Endothelial cells were derived from 4 tissues: iris, retina, choroid and human umbilical vein. RNA extracts from cells were hybridised to Affymetrix HGU133plus2 arrays in triplicate.





A 4 column table relating each sample (see above list) to parts of the body. The columns (parts of the body) are the following:


Geo Accession #Series GSE20986


Browning, A. C., Halligan, E. P., Swan, D. C., et al. (Jan 2012) “Comparative Gene Expression Profiling of HUVEC and Ocular Vascular Endothelial Cells.” Br J Ophthalmol. Vol. 96, No. 1, p. 128–132. doi: 10.1136/bjophthalmol-2011-300572.

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