This data set was used in Vandesompele et al (2002) to demonstrate normalization of real-time quantitative RT-PCR data by geometric averaging of housekeeping genes.
A data frame with 85 observations on the following 10 variables which stand for expression data of ten commonly used housekeeping genes
hypoxanthine phosphoribosyltransferase 1
ribosomal protein L13a
succinate dehydrogenase complex subunit A
TATA box binding protein
tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide
The row names of this data set indicate the various human tissues which were investigated.
9 normal bone-marrow samples
9 normal human tissues from pooled organs (heart, brain, fetal brain, lung, trachea, kidney, mammary gland, small intestine and uterus)
20 short-term cultured normal fibroblast samples from different individuals
13 normal leukocyte samples
34 neuroblastoma cell lines (independently prepared in different labs from different patients)
The data set was obtained from http://genomebiology.com/content/supplementary/gb-2002-3-7-research0034-s1.txt
Jo Vandesompele, Katleen De Preter, Filip Pattyn et al. (2002). Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biology 2002. 3(7):research0034.1-0034.11. http://genomebiology.com/2002/3/7/research/0034/
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