The Delta-Delta-Ct (ddCt) Algorithm is an approximation method to determine relative gene expression with quantitative real-time PCR (qRT-PCR) experiments. Compared to other approaches, it requires no standard curve for each primer-target pair, therefore reducing the working load and yet returning accurate enough results as long as the assumptions of the amplification efficiency hold. The ddCt package implements a pipeline to collect, analyse and visualize qRT-PCR results, for example those from TaqMan SDM software, mainly using the ddCt method. The pipeline can be either invoked by a script in command-line or through the API consisting of S4-Classes, methods and functions.
|Author||Jitao David Zhang, Rudolf Biczok, and Markus Ruschhaupt|
|Bioconductor views||DifferentialExpression GeneExpression MicrotitrePlateAssay qPCR|
|Maintainer||Jitao David Zhang <[email protected]>|
|Package repository||View on GitHub|
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