Bioconductor-mirror/dyebias: The GASSCO method for correcting for slide-dependent gene-specific dye bias

Many two-colour hybridizations suffer from a dye bias that is both gene-specific and slide-specific. The former depends on the content of the nucleotide used for labeling; the latter depends on the labeling percentage. The slide-dependency was hitherto not recognized, and made addressing the artefact impossible. Given a reasonable number of dye-swapped pairs of hybridizations, or of same vs. same hybridizations, both the gene- and slide-biases can be estimated and corrected using the GASSCO method (Margaritis et al., Mol. Sys. Biol. 5:266 (2009), doi:10.1038/msb.2009.21)

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Package details

AuthorPhilip Lijnzaad and Thanasis Margaritis
Bioconductor views Microarray Preprocessing QualityControl TwoChannel
MaintainerPhilip Lijnzaad <[email protected]>
Package repositoryView on GitHub
Installation Install the latest version of this package by entering the following in R:
Bioconductor-mirror/dyebias documentation built on June 1, 2017, 7:37 a.m.