Retrieve a data frame of index sorted data and sort indices from an FCS file.
The input FCS file should already be compensated.
Index sorting permits association of cell-level fluorescence intensities with
downstream data collection on the sorted cells. Cells are sorted into a plate with
coordinates, and those coordinates are stored in the FCS file.
This function will extract the data frame of flow data and the
X,Y coordinates for the
cell-level data, which can be written to a text file, or concatenated with sample-level
information and analyzed in R. The coordinates are names 'XLoc','YLoc', and a 'name' column is
also prepended with the FCS file name.
Matrix of fluorescence intensities and sort indices for plate location. When no index sorting data is available, invisibly returns 0. Test for 0 to check success.
Return a matrix of fluorescence intensities and indices into the sorting plate for each cell.
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