inst/app/contributions/gQC_generalQC/outputs.R
In C3BI-pasteur-fr/UTechSCB-SCHNAPPs: Single Cell Shiny Application for Analysing Single Cell Transcriptomics Data
# generalQC outputs.R
# source("moduleServer.R", local = TRUE)
# source("reactives.R", local = TRUE)
# TODO: verify that this anything and then integrate in DUMMY
myZippedReportFiles <- c("gqcProjections.csv")
require(stringr)
.schnappsEnv$gQC_X1 <- "tsne1"
.schnappsEnv$gQC_X2 <- "tsne2"
.schnappsEnv$gQC_X3 <- "tsne3"
.schnappsEnv$gQC_col <- "sampleNames"
observe(label = "ob2", {
if (DEBUG) cat(file = stderr(), "observe: gQC_dim3D_x\n")
.schnappsEnv$gQC_X1 <- input$gQC_dim3D_x
})
observe(label = "ob3", {
if (DEBUG) cat(file = stderr(), "observe: gQC_dim3D_y\n")
.schnappsEnv$gQC_X2 <- input$gQC_dim3D_y
})
observe(label = "ob4", {
if (DEBUG) cat(file = stderr(), "observe: gQC_dim3D_z\n")
.schnappsEnv$gQC_X3 <- input$gQC_dim3D_z
})
observe(label = "ob5", {
if (DEBUG) cat(file = stderr(), "observe: gQC_col3D\n")
.schnappsEnv$gQC_col <- input$gQC_col3D
})
observe(label = "obs_gQC_tsnePerplexity", x = {
.schnappsEnv$defaultValues[["gQC_tsnePerplexity"]] = input$gQC_tsnePerplexity
})
observe(label = "obs_gQC_tsneSeed", x = {
.schnappsEnv$defaultValues[["gQC_tsneSeed"]] = input$gQC_tsneSeed
})
observe(label = "obs_gQC_tsneTheta", x = {
.schnappsEnv$defaultValues[["gQC_tsneTheta"]] = input$gQC_tsneTheta
})
observe(label = "obs_gQC_tsneDim", x = {
.schnappsEnv$defaultValues[["gQC_tsneDim"]] = input$gQC_tsneDim
})
observe(label = "obs_gQC_dim3D_x", x = {
.schnappsEnv$defaultValues[["gQC_dim3D_x"]] = input$gQC_dim3D_x
})
observe(label = "obs_gQC_dim3D_y", x = {
.schnappsEnv$defaultValues[["gQC_dim3D_y"]] = input$gQC_dim3D_y
})
observe(label = "obs_gQC_dim3D_z", x = {
.schnappsEnv$defaultValues[["gQC_dim3D_z"]] = input$gQC_dim3D_z
})
observe(label = "obs_gQC_col3D", x = {
.schnappsEnv$defaultValues[["gQC_col3D"]] = input$gQC_col3D
})
observe(label = "obs_gQC_um_spread", x = {
.schnappsEnv$defaultValues[["gQC_um_spread"]] = input$gQC_um_spread
})
observe(label = "obs_gQC_um_n_components", x = {
.schnappsEnv$defaultValues[["gQC_um_n_components"]] = input$gQC_um_n_components
})
observe(label = "obs_gQC_um_n_neighbors", x = {
.schnappsEnv$defaultValues[["gQC_um_n_neighbors"]] = input$gQC_um_n_neighbors
})
observe(label = "obs_gQC_um_init", x = {
.schnappsEnv$defaultValues[["gQC_um_init"]] = input$gQC_um_init
})
observe(label = "obs_gQC_um_negative_sample_rate", x = {
.schnappsEnv$defaultValues[["gQC_um_negative_sample_rate"]] = input$gQC_um_negative_sample_rate
})
observe(label = "obs_gQC_um_randSeed", x = {
.schnappsEnv$defaultValues[["gQC_um_randSeed"]] = input$gQC_um_randSeed
})
observe(label = "obs_gQC_um_local_connectivity", x = {
.schnappsEnv$defaultValues[["gQC_um_local_connectivity"]] = input$gQC_um_local_connectivity
})
observe(label ="obs_gQC_um_bandwidth", x = {
.schnappsEnv$defaultValues[["gQC_um_bandwidth"]] = input$gQC_um_bandwidth
})
observe(label ="obs_gQC_um_n_epochs", x = {
.schnappsEnv$defaultValues[["gQC_um_n_epochs"]] = input$gQC_um_n_epochs
})
observe(label ="obs_gQC_um_set_op_mix_ratio", x = {
.schnappsEnv$defaultValues[["gQC_um_set_op_mix_ratio"]] = input$gQC_um_set_op_mix_ratio
})
observe(label ="obs_gQC_um_metric", x = {
.schnappsEnv$defaultValues[["gQC_um_metric"]] = input$gQC_um_metric
})
observe(label ="obs_gQC_um_min_dist", x = {
.schnappsEnv$defaultValues[["gQC_um_min_dist"]] = input$gQC_um_min_dist
})
observe(label ="obs_gQC_binSize", x = {
.schnappsEnv$defaultValues[["gQC_binSize"]] = input$gQC_binSize
})
# gQC_update3DInput ----
#' gQC_update3DInput
#' update axes for tsne display
gQC_update3DInput <- observeEvent(projections(),{
if (DEBUG) cat(file = stderr(), "gQC_update3DInput started.\n")
start.time <- base::Sys.time()
on.exit({
printTimeEnd(start.time, "gQC_update3DInput")
if (!is.null(getDefaultReactiveDomain())) {
removeNotification(id = "gQC_update3DInput")
}
})
if (!is.null(getDefaultReactiveDomain())) {
showNotification("gQC_update3DInput", id = "gQC_update3DInput", duration = NULL)
}
projections <- projections()
# Can use character(0) to remove all choices
if (is.null(projections)) {
return(NULL)
}
# choices = colnames(projections)[unlist(lapply(colnames(projections), function(x) !is.factor(projections[,x])))]
choices <- colnames(projections)
# Can also set the label and select items
updateSelectInput(session, "gQC_dim3D_x",
choices = choices,
selected = .schnappsEnv$gQC_X1
)
updateSelectInput(session, "gQC_dim3D_y",
choices = choices,
selected = .schnappsEnv$gQC_X2
)
updateSelectInput(session, "gQC_dim3D_z",
choices = choices,
selected = .schnappsEnv$gQC_X3
)
updateSelectInput(session, "gQC_col3D",
choices = colnames(projections),
selected = .schnappsEnv$gQC_col
)
})
# observer of UMAP button ----
observe(label = "ob_UMAPParams", {
# save(file = "~/SCHNAPPsDebug/ob_UMAPParams.RData", list = c(ls(), ".schnappsEnv"))
# load("~/SCHNAPPsDebug/updateButtonColor.RData")
deepDebug()
if (DEBUG) cat(file = stderr(), "observe umapVars\n")
input$activateUMAP
setRedGreenButtonCurrent(
vars = list(
c("gQC_um_randSeed", input$gQC_um_randSeed),
c("gQC_um_n_neighbors", input$gQC_um_n_neighbors),
c("gQC_um_n_components", input$gQC_um_n_components),
c("gQC_um_n_epochs", input$gQC_um_n_epochs),
# c("um_alpha", input$um_alpha),
c("gQC_um_init", input$gQC_um_init),
c("gQC_um_min_dist", input$gQC_um_min_dist),
c("gQC_um_set_op_mix_ratio", input$gQC_um_set_op_mix_ratio),
c("gQC_um_local_connectivity", input$gQC_um_local_connectivity),
c("gQC_um_bandwidth", input$gQC_um_bandwidth),
c("um_gamma", input$um_gamma),
c("gQC_um_negative_sample_rate", input$gQC_um_negative_sample_rate),
c("gQC_um_metric", input$gQC_um_metric),
c("gQC_um_spread", input$gQC_um_spread)
)
)
updateButtonColor(buttonName = "activateUMAP", parameters = c(
"gQC_um_randSeed", "gQC_um_n_neighbors", "gQC_um_n_components", "gQC_um_n_epochs",
"gQC_um_init", "gQC_um_min_dist", "gQC_um_set_op_mix_ratio",
"gQC_um_local_connectivity", "gQC_um_bandwidth", "um_gamma",
"gQC_um_negative_sample_rate", "gQC_um_metric", "gQC_um_spread"
))
})
## observe coE_updateInputXviolinPlot ----
observe({
if (DEBUG) cat(file = stderr(), "coE_updateInputXviolinPlot started.\n")
start.time <- base::Sys.time()
on.exit({
printTimeEnd(start.time, "coE_updateInputXviolinPlot")
if (!is.null(getDefaultReactiveDomain())) {
removeNotification(id = "coE_updateInputXviolinPlot")
}
})
if (!is.null(getDefaultReactiveDomain())) {
showNotification("coE_updateInputXviolinPlot", id = "coE_updateInputXviolinPlot", duration = NULL)
}
tsneData <- projections()
projFactors <- projFactors()
# Can use character(0) to remove all choices
if (is.null(tsneData)) {
return(NULL)
}
updateSelectInput(
session,
"coE_dimension_xVioiGrp",
choices = projFactors,
selected = .schnappsEnv$coE_dimension_xVioiGrp
)
updateSelectInput(
session,
"coE_scranFactor",
choices = projFactors
)
updateSelectInput(
session,
"coE_dimension_xVioiGrp2",
choices = projFactors,
selected = .schnappsEnv$coE_dimension_xVioiGrp2
)
})
# observe: cellNameTable_rows_selected ----
observe(label = "ob_tsneParams", {
deepDebug()
if (DEBUG) cat(file = stderr(), "observe tsneVars\n")
out <- tsne()
if (is.null(out)) {
.schnappsEnv$calculated_gQC_tsneDim <- "NA"
}
input$updatetsneParameters
setRedGreenButtonCurrent(
vars = list(
c("gQC_tsneDim", input$gQC_tsneDim),
c("gQC_tsnePerplexity", input$gQC_tsnePerplexity),
c("gQC_tsneTheta", input$gQC_tsneTheta),
c("gQC_tsneSeed", input$gQC_tsneSeed)
)
)
updateButtonColor(buttonName = "updatetsneParameters", parameters = c(
"gQC_tsneDim", "gQC_tsnePerplexity",
"gQC_tsneTheta", "gQC_tsneSeed"
))
})
# gQC_tsne_main ----
output$gQC_tsne_main <- plotly::renderPlotly({
if (DEBUG) cat(file = stderr(), "gQC_tsne_main started.\n")
start.time <- base::Sys.time()
on.exit({
printTimeEnd(start.time, "gQC_tsne_main")
if (!is.null(getDefaultReactiveDomain())) {
removeNotification(id = "gQC_tsne_main")
}
})
if (!is.null(getDefaultReactiveDomain())) {
showNotification("gQC_tsne_main", id = "gQC_tsne_main", duration = NULL)
}
# upI <- gQC_update3DInput()
projections <- projections()
dimX <- input$gQC_dim3D_x
dimY <- input$gQC_dim3D_y
dimZ <- input$gQC_dim3D_z
dimCol <- input$gQC_col3D
# scols <- projectionColors$sampleNames
# ccols <- clusterCols$colPal
pc = projectionColors %>% reactiveValuesToList()
if (is.null(projections)) {
if (DEBUG) cat(file = stderr(), "output$gQC_tsne_main:NULL\n")
return(NULL)
}
if (.schnappsEnv$DEBUGSAVE) {
save(file = normalizePath("~/SCHNAPPsDebug/gQC_tsne_main.RData"), list = c(ls()))
}
# cp =load(file="~/SCHNAPPsDebug/gQC_tsne_main.RData")
retVal <- tsnePlot(projections, dimX, dimY, dimZ, dimCol, projColors = pc)
exportTestValues(tsnePlot = {
str(retVal)
})
layout(retVal)
})
# gQC_umap_main 2D plot ----
callModule(
clusterServer,
"gQC_umap_main",
projections
)
# gQC_projectionTableMod ----
callModule(
tableSelectionServer,
"gQC_projectionTableMod",
projectionTable, caption = "Table with projections"
)
# gQC_projectionCombTableMod ----
callModule(
tableSelectionServer,
"gQC_projCombTableMod",
projectionTable, caption = "Tables with projections"
)
# gQC_plotUmiHist ----
output$gQC_plotUmiHist <- plotly::renderPlotly({
if (DEBUG) cat(file = stderr(), "gQC_plotUmiHist started.\n")
start.time <- base::Sys.time()
on.exit({
printTimeEnd(start.time, "gQC_plotUmiHist")
if (!is.null(getDefaultReactiveDomain())) {
removeNotification(id = "gQC_plotUmiHist")
}
})
if (!is.null(getDefaultReactiveDomain())) {
showNotification("gQC_plotUmiHist", id = "gQC_plotUmiHist", duration = NULL)
}
scEx <- scEx()
pc = projectionColors %>% reactiveValuesToList()
scols <- projectionColors$sampleNames
binSize <- input$gQC_binSize
if (is.null(scEx)) {
return(NULL)
}
if (.schnappsEnv$DEBUGSAVE) {
save(file = normalizePath("~/SCHNAPPsDebug/gQC_plotUmiHist.RData"), list = c(ls()))
}
# cp = load(file = "~/SCHNAPPsDebug/gQC_plotUmiHist.RData")
dat <- data.frame(counts = Matrix::colSums(assays(scEx)[["counts"]]))
dat$sample <- colData(scEx)$sampleNames
fig <- plotly::plot_ly(alpha = 1,
nbinsx = binSize)
# dat[dat$sample == levels(colData(scEx)$sampleNames)[[1]],],
# x = ~counts,
# # y = ~counts,
# type="histogram")
lev = levels(colData(scEx)$sampleNames)
for (idx in seq_along(lev)) {
fig <- fig %>% add_trace(
type = 'histogram', color = I(scols[idx]), name = lev[idx],
x = dat[dat$sample == levels(colData(scEx)$sampleNames)[[idx]],"counts"]
)
}
fig <- fig %>% layout(
barmode="stack",
bargap=0.1,
title = "Histogram of UMIs",
yaxis = list(title = "Number of cells"),
xaxis = list(title = "UMI count"))
fig
# marker = list(color = scols))
# retVal <- ggplot(data = dat, aes(counts, fill = sample)) +
# geom_histogram(bins = 50) +
# labs(title = "Histogram for raw counts", x = "count", y = "Frequency") +
# scale_fill_manual(values = scols, aesthetics = "fill")
#
.schnappsEnv[["gQC_plotUmiHist"]] <- fig
return(fig)
})
# gQC_plotSampleHist -----
output$gQC_plotSampleHist <- plotly::renderPlotly({
if (DEBUG) cat(file = stderr(), "gQC_plotSampleHist started.\n")
start.time <- base::Sys.time()
on.exit({
printTimeEnd(start.time, "gQC_plotSampleHist")
if (!is.null(getDefaultReactiveDomain())) {
removeNotification(id = "gQC_plotSampleHist")
}
})
if (!is.null(getDefaultReactiveDomain())) {
showNotification("gQC_plotSampleHist", id = "gQC_plotSampleHist", duration = NULL)
}
sampleInf <- sampleInfo()
scols <- projectionColors$sampleNames
if (is.null(sampleInf)) {
return(NULL)
}
if (.schnappsEnv$DEBUGSAVE) {
save(file = normalizePath("~/SCHNAPPsDebug/sampleHist.RData"), list = c(ls()))
}
# cp = load(file = "~/SCHNAPPsDebug/sampleHist.RData")
retVal <- gQC_sampleHistFunc(sampleInf, scols)
.schnappsEnv[["gQC_plotSampleHist"]] <- retVal
return(retVal)
})
output$gQC_variancePCA <- renderPlot({
if (DEBUG) cat(file = stderr(), "gQC_variancePCA started.\n")
start.time <- base::Sys.time()
on.exit({
printTimeEnd(start.time, "gQC_variancePCA")
if (!is.null(getDefaultReactiveDomain())) {
removeNotification(id = "gQC_variancePCA")
}
})
if (!is.null(getDefaultReactiveDomain())) {
showNotification("gQC_variancePCA", id = "gQC_variancePCA", duration = NULL)
}
pca <- pcaReact()
if (is.null(pca)) {
return(NULL)
}
if (.schnappsEnv$DEBUGSAVE) {
save(file = normalizePath("~/SCHNAPPsDebug/gQC_variancePCA.RData"), list = c(ls()))
}
# load(file = "~/SCHNAPPsDebug/gQC_variancePCA.RData")
# h2("Variances of PCs")
df <- data.frame(var = pca$var_pcs, pc = 1:length(pca$var_pcs))
retVal <- plotHistVarPC(df, pc, var)
af = plotHistVarPC
# remove env because it is too big
environment(af) = new.env(parent = emptyenv())
.schnappsEnv[["gQC_variancePCA"]] <- list(plotFunc = af,
df, pc, var)
# .schnappsEnv[["gQC_variancePCA"]] <- retVal
return(retVal)
# barplot(pca$var_pcs, main = "Variance captured by first PCs")
})
# gene set related -----
## modify a gene set ----
observeEvent(input$geneSetModifyButton,{
if (DEBUG) cat(file = stderr(), "geneSetModifyButton\n")
start.time <- base::Sys.time()
on.exit({
printTimeEnd(start.time, "geneSetModifyButton")
if (!is.null(getDefaultReactiveDomain())) {
removeNotification(id = "geneSetModifyButton")
}
})
# show in the app that this is running
if (!is.null(getDefaultReactiveDomain())) {
showNotification("geneSetModifyButton", id = "geneSetModifyButton", duration = NULL)
}
# inputGS <- input$gQC_geneSetModifyInput
newName = make.names(input$gQC_geneSetModifynName)
newGenes = input$gQC_geneSetModifyGenes
desc = input$gQC_geneSetModifynDesc
gd = gmtData()
userData = gmtUserData()
scEx = scEx()
featureData <- rowData(scEx)
# browser()
# deepDebug()
if (.schnappsEnv$DEBUGSAVE) {
save(file = normalizePath("~/SCHNAPPsDebug/geneSetModifyButton.RData"), list = c(ls()))
}
# cp = load(file="~/SCHNAPPsDebug/geneSetModifyButton.RData")
if (newGenes == "" | newName == "X" | is.null(scEx)) {
return(NULL)
}
# li = new gene list
li <- geneName2Index(g_id = newGenes, featureData = featureData)
if(is.null(li)) {
cat(file = stderr(), "!!!!geneSetModifyButton: no genes found\n")
return(NULL)
}
if(newName %in% names(gd)) {
cat(file = stderr(), "!!!!geneSetModifyButton: gene set already set.\n")
return(NULL)
}
li = list(list(genes = featureData[li,"symbol"], desc = desc, name = newName))
names(li) = newName
# append to global list
if(is.null(userData)){
gmtUserData(li)
} else {
gmtUserData(append(userData, li))
}
.schnappsEnv$defaultValues["gQC_geneSetModifyInput"] = newName
updateSelectizeInput(
session = session,
inputId = "gQC_geneSetModifyInput",
# choices = ,
selected = newName
)
})
# GSEA ----
output$gQC_geneSetsearchOutput = renderText({
if (DEBUG) cat(file = stderr(), "geneSetsearchOutput\n")
start.time <- base::Sys.time()
on.exit({
printTimeEnd(start.time, "geneSetsearchOutput")
if (!is.null(getDefaultReactiveDomain())) {
removeNotification(id = "geneSetsearchOutput")
}
})
# show in the app that this is running
if (!is.null(getDefaultReactiveDomain())) {
showNotification("geneSetsearchOutput", id = "geneSetsearchOutput", duration = NULL)
}
# inputGS <- input$gQC_geneSetModifyInput
genes = input$gQC_genesets_search
maxItems = isolate(input$gQC_genesets_maxPrint)
gd = gmtData()
userData = gmtUserData()
scEx = scEx()
featureData <- rowData(scEx)
# browser()
# deepDebug()
if (.schnappsEnv$DEBUGSAVE) {
save(file = normalizePath("~/SCHNAPPsDebug/geneSetsearchOutput.RData"), list = c(ls()))
}
# cp = load(file="~/SCHNAPPsDebug/geneSetsearchOutput.RData")
if ( is.null(scEx)) {
return(NULL)
}
# li = new gene list
li <- geneName2Index(g_id = genes, featureData = featureData)
if(is.null(li)) {
cat(file = stderr(), "!!!!geneSetsearchOutput: no genes found\n")
return(NULL)
}
# parallel bplapply on gmtData -- fast enough
counts = bplapply(gd, FUN=function(x)sum(li %in% x$genes))
counts = counts[which(counts>0)]
outStr = ""
countNames = counts %>% unlist() %>% sort(decreasing = T) %>% names()
maxItems = min(maxItems, length(countNames))
for (name in countNames[1:maxItems]){
outStr = paste(outStr, name, "found:", counts[[name]], " of ", length(gd[[name]]$genes),"\n",
gd[[name]]$desc, "\n",
paste(li[which(li %in% gd[[name]]$genes)], collapse=", "), "\n",
paste(gd[[name]]$genes, collapse=", "), "\n\n")
}
outStr
})
# %>% bindCache(input$gQC_genesets_search, gmtData())
# gQC_renameGenes ----
output$gQC_renameGenes <- renderText({
if (DEBUG) cat(file = stderr(), "gQC_renameGenes\n")
start.time <- base::Sys.time()
on.exit({
printTimeEnd(start.time, "gQC_renameGenes")
if (!is.null(getDefaultReactiveDomain())) {
removeNotification(id = "gQC_renameGenes")
}
})
# show in the app that this is running
if (!is.null(getDefaultReactiveDomain())) {
showNotification("gQC_renameGenes", id = "gQC_renameGenes", duration = NULL)
}
gd = gmtData()
inputGS <- input$oldGS
req(gd)
if (inputGS == "") {
return(NULL)
}
# deepDebug()
if (.schnappsEnv$DEBUGSAVE) {
save(file = normalizePath("~/SCHNAPPsDebug/gQC_renameGenes.RData"), list = c(ls()))
}
# cp = load(file="~/SCHNAPPsDebug/gQC_renameGenes.RData")
if(inputGS %in% names(gd)) {
retVal = paste(gd[[inputGS]]$genes, collapse = ", ")
} else {
retVal = ""
}
return(retVal)
})
output$gQC_geneSetModifyInputGL <- renderText({
if (DEBUG) cat(file = stderr(), "gQC_geneSetModifyInputGL\n")
start.time <- base::Sys.time()
on.exit({
printTimeEnd(start.time, "gQC_geneSetModifyInputGL")
if (!is.null(getDefaultReactiveDomain())) {
removeNotification(id = "gQC_geneSetModifyInputGL")
}
})
# show in the app that this is running
if (!is.null(getDefaultReactiveDomain())) {
showNotification("gQC_geneSetModifyInputGL", id = "gQC_geneSetModifyInputGL", duration = NULL)
}
gd = gmtData()
inputGS <- input$gQC_geneSetModifyInput
req(gd)
if (inputGS == "") {
return(NULL)
}
# deepDebug()
if (.schnappsEnv$DEBUGSAVE) {
save(file = normalizePath("~/SCHNAPPsDebug/gQC_geneSetModifyInput.RData"), list = c(ls()))
}
# cp = load(file="~/SCHNAPPsDebug/gQC_geneSetModifyInput.RData")
if(inputGS %in% names(gd)) {
retVal = paste(gd[[inputGS]]$genes, collapse = ", ")
} else {
retVal = ""
}
return(retVal)
})
observe({
gd = gmtData()
updateSelectizeInput(session, inputId = "oldGS",choices = names(gd), server = TRUE)
updateSelectizeInput(session, inputId = "gQC_geneSetModifyInput",
choices = names(gd),
selected = defaultValue("gQC_geneSetModifyInput", "dummy"), server = TRUE)
})
observeEvent(input$updateGSButton,{
userData = gmtUserData()
gd = gmtData()
name = input$oldGS
newName = input$newGS
if(!name %in% names(gd)) return(NULL)
li = gd[name]
names(li) = newName
gmtUserData(append(userData, li))
if(.schnappsEnv$DEBUGSAVE){
save(file = normalizePath("~/SCHNAPPsDebug/updateGSButton.RData"), list = c(ls()))
}
# cp =load(file='~/SCHNAPPsDebug/updateGSButton.RData')
})
# rename projections observers ----
observeEvent(
label = "ob30",
eventExpr = input$updatePrjsButton,
handlerExpr = {
deepDebug()
if (DEBUG) cat(file = stderr(), "updatePrjsButton\n")
oldPrj <- input$oldPrj
newPrj <- input$newPrj
projections <- projections()
acn = allCellNames()
newPrjs <- projectionsTable$newProjections
if (is.null(projections)) {
return(NULL)
}
if (.schnappsEnv$DEBUGSAVE) {
save(file = normalizePath("~/SCHNAPPsDebug/updatePrjsButton.RData"),
list = c("normaliztionParameters", ls())
)
}
# cp = load(file="~/SCHNAPPsDebug/updatePrjsButton.RData")
# deepDebug()
if (newPrj %in% colnames(projections)) {
showNotification(
"New column name already used",
type = "error",
duration = NULL
)
return(NULL)
}
if (ncol(newPrjs) == 0) {
newPrjs = data.frame(row.names = acn)
newPrjs[,newPrj] = NA
newPrjs[rownames(projections),newPrj] <- projections[, oldPrj, drop = FALSE]
} else {
# newPrjs <- cbind(newPrjs[rownames(projections), , drop = FALSE], projections[, oldPrj, drop = FALSE])
# deepDebug()
newPrjs <- dplyr::full_join(
tibble::rownames_to_column(newPrjs),
tibble::rownames_to_column(projections[, oldPrj, drop = FALSE]),
by='rowname')
rownames(newPrjs) = newPrjs[,1]
newPrjs = newPrjs[,-1]
}
colnames(newPrjs)[ncol(newPrjs)] <- newPrj
if(is.factor(projections[, oldPrj])) newPrjs[,newPrj] = as.factor(newPrjs[,newPrj])
projectionsTable$newProjections <- newPrjs
}
)
# # rename projections
# observe(label = "ob27", {
# projections <- projections()
#
# })
observe(label = "ob28", {
deepDebug()
input$newPrj
updateTextInput(session, "newPrj", value = make.names(input$newPrj, unique = TRUE))
})
observeEvent(
label = "ob29",
eventExpr = input$delPrjsButton,
handlerExpr = {
deepDebug()
if (DEBUG) cat(file = stderr(), "updatePrjsButton\n")
newPrjs <- projectionsTable$newProjections
delPrj <- input$delPrj
if (is.null(projections)) {
return(NULL)
}
if (!delPrj %in% colnames(newPrjs)) {
return(NULL)
}
# deepDebug()
if (.schnappsEnv$DEBUGSAVE) {
save(file = normalizePath("~/SCHNAPPsDebug/delPrjsButton.RData"),
list = c("normaliztionParameters", ls())
)
}
# load(file="~/SCHNAPPsDebug/delPrjsButton.RData")
projectionsTable$newProjections <- newPrjs[, -which(colnames(newPrjs) == delPrj), drop = FALSE]
}
)
# combine projections observers ----
observeEvent(
label = "gQC_updateCombPrjsButton",
eventExpr = input$gQC_updateCombPrjsButton,
handlerExpr = {
if (DEBUG) cat(file = stderr(), "gQC_updateCombPrjsButton\n")
prj1 <- input$gQC_combPrj1
prj2 <- input$gQC_combPrj2
newPrj <- make.names(input$gQC_newCombPrj)
projections <- projections()
newPrjs <- projectionsTable$newProjections
acn = allCellNames()
if (is.null(projections)) {
return(NULL)
}
if (!all(c(prj1, prj2) %in% colnames(projections))) {
return(NULL)
}
if (.schnappsEnv$DEBUGSAVE) {
save(file = normalizePath("~/SCHNAPPsDebug/gQC_updateCombPrjsButton.RData"),
list = c("normaliztionParameters", ls())
)
}
# cp= load(file="~/SCHNAPPsDebug/gQC_updateCombPrjsButton.RData")
if (newPrj %in% colnames(projections)) {
showNotification(
"New column name already used",
type = "error",
duration = NULL
)
return(NULL)
}
# deepDebug()
combProjections = data.frame(row.names = rownames(projections),
newPrj= paste(projections[,prj1], projections[,prj2], sep = " - ") %>% as.factor())
if (length(levels(combProjections[,1])) > 100) {
out = showModal(verifyLevelModal(NLevel = length(levels(combProjections[,1]))))
if (DEBUG) cat(file = stderr(), paste("gQC_updateCombPrjsButton modal out:", out, "\n"))
# deepDebug()
}
if (ncol(newPrjs) == 0) {
newPrjs <- data.frame(row.names = acn)
newPrjs[rownames(combProjections),newPrj] = combProjections[,1]
# rownames(newPrjs) = rownames(projections)
} else {
# newPrjs <- cbind(newPrjs[rownames(projections), , drop = FALSE], combProjections)
# deepDebug()
newPrjs <- dplyr::left_join(
tibble::rownames_to_column(newPrjs),
tibble::rownames_to_column(combProjections),
by='rowname')
rownames(newPrjs) = newPrjs[,1]
newPrjs = newPrjs[,-1]
}
colnames(newPrjs)[ncol(newPrjs)] <- newPrj
projectionsTable$newProjections <- newPrjs
}
)
# output$gQC_orgLevels ----
output$gQC_orgLevels = renderText({
rnProj = input$gQC_rnProj
projections = projections()
shiny::req(rnProj)
shiny::req(projections)
if (! rnProj %in% colnames(projections)) return(NULL)
# deepDebug()
paste(levels(factor(projections[,rnProj])), collapse = ", ")
})
# rename projection levels ----
verifyLevelModal <- function(NLevel, failed = FALSE) {
modalDialog(
span(paste(
"There are ", NLevel, "new levels, are you sure you want to do this?\n")
)
,
footer = tagList(
modalButton("Cancel"),
actionButton("commentok", "OK")
)
)
}
# gQC_rearrange levels ----
observeEvent(eventExpr = input$gQC_raProj,
label = "raLevBtn",
handlerExpr = {
deepDebug()
projections <- projections()
projFactors <- projFactors()
if(is.null(projections)) return()
if(is.null(projFactors)) return()
if(input$gQC_raProj %in% projFactors){
projLevels = levels(projections[,input$gQC_raProj])
updateOrderInput(
session,
'gQC_newRaLev',
items = projLevels
)
}else{
updateOrderInput(
session,
'gQC_newRaLev',
items = "not yet"
)
}
}
)
observeEvent(eventExpr = input$gQC_rearrangeLevButton,
label = "raLevBtn",
handlerExpr = {
deepDebug()
newProjName = make.names(input$gQC_newRaPrj)
newLevelOrder = input$gQC_newRaLev
projections = projections()
raProj = input$gQC_raProj
acn = allCellNames()
newPrjs <- projectionsTable$newProjections
if (is.null(projections)) {
return(NULL)
}
if (.schnappsEnv$DEBUGSAVE) {
save(file = normalizePath("~/SCHNAPPsDebug/gQC_rearrangeButton.RData"),
list = c("normaliztionParameters", ls())
)
}
# cp= load(file="~/SCHNAPPsDebug/gQC_rearrangeButton.RData")
orgLevelNames = levels(factor(projections[,raProj]))
newLbVec = stringr::str_trim(str_split(newLevelOrder, ","))
# names(newLbVec) = orgLevelNames
# deepDebug()
# sampe projections as displayed, i.e. only those available for the cells
# otherwise the diplay (output$gQC_orgLevels) has to be changed as well
projections[,raProj] = factor(projections[,raProj])
if(is.null(
tryCatch({
if (ncol(newPrjs) == 0) {
newPrjs = data.frame(row.names = acn)
newPrjs[,newProjName] = "NA"
# drop = TRUE: we re interested in the vector not the data frame
newPrjs[rownames(projections),newProjName] <- as.character(projections[, raProj, drop = TRUE])
} else {
# deepDebug()
newPrjs <- dplyr::full_join(
tibble::rownames_to_column(newPrjs),
tibble::rownames_to_column(projections[, raProj, drop = FALSE]),
by='rowname')
rownames(newPrjs) = newPrjs[,1]
newPrjs = newPrjs[,-1]
# newPrjs <- cbind(newPrjs[rownames(projections), , drop = FALSE], projections[,raProj])
}
newPrjs[,ncol(newPrjs)] = factor(newPrjs[,ncol(newPrjs)], levels = newLbVec)
# # in case there was NA introduced by hidden cells
# if ("NA" %in% levels(newPrjs[,ncol(newPrjs)]) & !"NA" %in% newLevelOrder ){
# newLevelOrder = c(newLevelOrder, "NA")
# }
# if (!length(levels(newPrjs[,ncol(newPrjs)])) == length(newLevelOrder) ){
# cat(file = stderr(), paste("number of levels not correct\n\nold levels:\n"))
# cat(file = stderr(), levels(newPrjs[,ncol(newPrjs)]))
# cat(file = stderr(), paste("\n\n\nnew levels:\n"))
# cat(file = stderr(), newLevelOrder)
# cat(file = stderr(), paste("\n"))
# showNotification("number of levels not correct. See console", id = "renameProbl", duration = NULL, type = "error")
# return(NULL)
# }
# newPrjs[,ncol(newPrjs)] = factor(newPrjs[,ncol(newPrjs)], levels = newLbVec)
}, error=function(w){
# deepDebug()
cat(file = stderr(), paste("something went wrong during releveling", w,"\n"))
showNotification("problem with names", id = "renameProbl", duration = NULL, type = "error")
return(NULL)
}))) return(NULL)
# newProjName = make.unique(c(colnames(projections),newProjName))[length(c(colnames(projections),newProjName))]
# updateTextInput(session, "gQC_newRnPrj", value = newProjName)
colnames(newPrjs)[ncol(newPrjs)] <- newProjName
projectionsTable$newProjections <- newPrjs
})
# gQC_renameLevButton ----
observeEvent(eventExpr = input$gQC_renameLevButton,
label = "rnLevBtn",
handlerExpr = {
deepDebug()
newLables = input$gQC_renameLev
rnProj = input$gQC_rnProj
newProjName = make.names(input$gQC_newRnPrj)
projections = projections()
acn = allCellNames()
newPrjs <- projectionsTable$newProjections
if (is.null(projections)) {
return(NULL)
}
orgLevelNames = levels(factor(projections[,rnProj]))
newLbVec = stringr::str_trim(str_split(newLables, ",")[[1]]) %>% make.names()
names(newLbVec) = orgLevelNames
if (.schnappsEnv$DEBUGSAVE) {
# browser()
save(file = normalizePath("~/SCHNAPPsDebug/gQC_renameLevButton.RData"),
list = c("normaliztionParameters", ls())
)
}
# cp= load(file="~/SCHNAPPsDebug/gQC_renameLevButton.RData")
# deepDebug()
# sampe projections as displayed, i.e. only those available for the cells
# otherwise the display (output$gQC_orgLevels) has to be changed as well
proj2Add = projections[,rnProj,drop=FALSE]
proj2Add[,rnProj] = factor(proj2Add[,rnProj])
if(is.null(
tryCatch({
# copy original data
# This can be the first newPrjs
if (ncol(newPrjs) == 0) {
newPrjs = data.frame(row.names = acn)
newPrjs[,newProjName] = "NA"
# drop = TRUE: we re interested in the vector not the data frame
newPrjs[rownames(projections),newProjName] <- as.character(projections[, rnProj, drop = TRUE])
} else {
# deepDebug()
newPrjs <- dplyr::full_join(
tibble::rownames_to_column(newPrjs),
tibble::rownames_to_column(projections[, rnProj, drop = FALSE]),
by='rowname')
rownames(newPrjs) = newPrjs[,1]
newPrjs = newPrjs[,-1]
# newPrjs <- cbind(newPrjs[rownames(projections), , drop = FALSE], projections[,rnProj])
}
# ensure that this is a factor
newPrjs[,ncol(newPrjs)] = as.factor(newPrjs[,ncol(newPrjs)])
if ("NA" %in% levels(newPrjs[,ncol(newPrjs)]) & !"NA" %in% stringr::str_trim(newLbVec) ){
newLevelNames = levels(newPrjs[,ncol(newPrjs)])
naPos = which ("NA" == newLevelNames)
newLbVec = newLbVec[newLevelNames]
newLbVec[which(is.na(newLbVec))] = "NA"
}
# if (!length(levels(newPrjs[,ncol(newPrjs)])) == length(stringr::str_trim(newLbVec)) ){
# cat(file = stderr(), paste("number of levels not correct\n\nold levels:\n"))
# cat(file = stderr(), levels(newPrjs[,ncol(newPrjs)]))
# cat(file = stderr(), paste("\n\n\nnew levels:\n"))
# cat(file = stderr(), stringr::str_trim(newLbVec))
# cat(file = stderr(), paste("\n"))
# showNotification("number of levels not correct. See console", id = "renameProbl", duration = NULL, type = "error")
# return(NULL)
# }
oldLevels = levels(newPrjs[,ncol(newPrjs)])
names(oldLevels) = oldLevels
oldLevels[names(newLbVec)] = stringr::str_trim(newLbVec)
levels(newPrjs[,ncol(newPrjs)]) = oldLevels
}, error=function(w){
# deepDebug()
cat(file = stderr(), paste("something went wrong during releveling", w,"\n"))
showNotification("problem with names", id = "renameProbl", duration = NULL, type = "error")
return(NULL)
}))) return(NULL)
newProjName = make.unique(c(colnames(projections),newProjName)) %>% tail(n=1)
colnames(newPrjs)[ncol(newPrjs)] <- newProjName
projectionsTable$newProjections <- newPrjs
updateTextInput(session, "gQC_newRnPrj", value = newProjName)
})
observeEvent(eventExpr = input$gQC_rnProj,
label = "gqc1",
handlerExpr = {
deepDebug()
rnProj = input$gQC_rnProj
projections = projections()
shiny::req(rnProj)
shiny::req(projections)
if (! rnProj %in% colnames(projections)) return(NULL)
# deepDebug()
updateTextAreaInput(session, inputId = "gQC_renameLev", value = paste(as.character(levels(factor(projections[,rnProj]))),
collapse = ", "))
})
# rename projections
.schnappsEnv$gQC_combPrj1 <- "tsne1"
.schnappsEnv$gQC_combPrj2 <- "tsne1"
.schnappsEnv$gQC_rnProj <- "tsne1"
observe(label = "ob27b", {
deepDebug()
projections <- projections()
projFactors <- projFactors()
# only factorials?
updateSelectInput(session, "gQC_raProj",
choices = projFactors,
selected = .schnappsEnv$gQC_raProj
)
updateSelectInput(session, "gQC_combPrj1",
choices = colnames(projections),
selected = .schnappsEnv$gQC_combPrj1
)
updateSelectInput(session, "gQC_combPrj2",
choices = colnames(projections),
selected = .schnappsEnv$gQC_combPrj2
)
updateSelectInput(session, "gQC_rnProj",
choices = colnames(projections),
selected = .schnappsEnv$gQC_rnProj
)
updateSelectInput(session, "oldPrj",
choices = c(colnames(projections)),
selected = .schnappsEnv$oldPrj
)
updateSelectInput(session, "delPrj",
choices = c(colnames(projectionsTable$newProjections)),
selected = .schnappsEnv$delPrj
)
updateSelectInput(session, "gQC_windProj",
choices = projFactors,
selected = .schnappsEnv$gQC_windProj
)
})
observe(label = "ob27c", {
if (DEBUG) cat(file = stderr(), "observe: gQC_combPrj1\n")
.schnappsEnv$gQC_combPrj1 <- input$gQC_combPrj1
})
observe(label = "ob27d", {
if (DEBUG) cat(file = stderr(), "observe: gQC_combPrj2\n")
.schnappsEnv$gQC_combPrj2 <- input$gQC_combPrj2
})
observe(label = "ob27e", {
if (DEBUG) cat(file = stderr(), "observe: gQC_rnProj\n")
.schnappsEnv$gQC_rnProj <- input$gQC_rnProj
})
observe(label = "ob27f", {
if (DEBUG) cat(file = stderr(), "observe: oldPrj\n")
.schnappsEnv$oldPrj <- input$oldPrj
})
observe(label = "ob27g", {
if (DEBUG) cat(file = stderr(), "observe: delPrj\n")
.schnappsEnv$delPrj <- input$delPrj
})
observe(label = "ob27h", {
if (DEBUG) cat(file = stderr(), "observe: gQC_windProj\n")
.schnappsEnv$gQC_windProj <- input$gQC_windProj
.schnappsEnv$defaultValues[["gQC_windProj"]] <- input$gQC_windProj
})
observe(label = "ob27i", {
if (DEBUG) cat(file = stderr(), "observe: gQC_raProj\n")
.schnappsEnv$gQC_raProj <- input$gQC_raProj
})
# rename levels
output$gQC_renameLev <- renderText({"text"})
# WIND ----
output$gQC_windHC <- renderPlot({
require(Wind)
# remotes::install_github("renozao/xbioc")
# library(xbioc)
if ("xbioc" %in% rownames(installed.packages())){
require(xbioc)
} else {
is_logscale <- function(x) {return(T)
cat(file = stderr(), "Please install xbioc: remotes::install_github('renozao/xbioc')")
}
}
if (DEBUG) cat(file = stderr(), "gQC_windHC started.\n")
start.time <- base::Sys.time()
on.exit({
printTimeEnd(start.time, "gQC_windHC")
if (!is.null(getDefaultReactiveDomain())) {
removeNotification(id = "gQC_windHC")
}
})
if (!is.null(getDefaultReactiveDomain())) {
showNotification("gQC_windHC", id = "gQC_windHC", duration = NULL)
}
scEx <- scEx()
projections <- projections()
pca = pcaReact()
gQC_windProj <- input$gQC_windProj
# browser()
if (is.null(projections) | is.null(scEx) | !gQC_windProj %in% colnames(projections)) {
return(NULL)
}
if (length(levels(projections[,gQC_windProj]))<3) {
if (!is.null(getDefaultReactiveDomain())) {
showNotification("Projections have less than 3 levels", id = "gQC_windHCPR", duration = 20, type = "warning")
}
}
if (.schnappsEnv$DEBUGSAVE) {
save(file = normalizePath("~/SCHNAPPsDebug/gQC_windHC.RData"), list = c(ls()))
}
# cp = load(file = "~/SCHNAPPsDebug/gQC_windHC.RData")
Y <- as.matrix(assays(scEx)[[1]])
# if(is_logscale(Y)) {
# Y = exp(Y)
# }
trueclass <- projections[,gQC_windProj]
ctStruct = tryCatch({
createRef(Y, classes = trueclass)
},error = function(e) {
if (!is.null(getDefaultReactiveDomain())) {
showNotification("Problem with WIND", type = "warning", duration = NULL)
}
cat(file = stderr(), paste("\n+++++ Error in WIND\n\t", e, "\n"))
return(NULL)
}
)
if(is.null(ctStruct)) return(NULL)
plot(ctStruct$hc, xlab="", axes=FALSE, ylab="", ann=FALSE)
})
# DoubletFinder related ----
##
if("DoubletFinder" %in% installed.packages()){
observe({
if (DEBUG) cat(file = stderr(), "GS_DF_pk update\n")
scEx = scEx()
updateNumericInput(session = session, inputId = "GS_DF_pk", value=20/ncol(scEx))
})
observeEvent(input$GS_DF_button,{
if (DEBUG) cat(file = stderr(), "GS_DF_button started.\n")
start.time <- base::Sys.time()
on.exit({
printTimeEnd(start.time, "GS_DF_button")
if (!is.null(getDefaultReactiveDomain())) {
removeNotification(id = "GS_DF_button")
}
})
if (!is.null(getDefaultReactiveDomain())) {
showNotification("GS_DF_button", id = "GS_DF_button", duration = NULL)
removeNotification(id = "GS_DF_buttonERROR")
}
scEx = scEx()
dims = input$GS_DF_dims
nRecover = input$GS_DF_nRecover
pK = input$GS_DF_pk
pN = input$GS_DF_pN
projections <- as.data.frame(projections)
newPrjs <- sessionProjections$prjs
acn = allCellNames()
if (is.null(scEx)) {
return(NULL)
}
if (is.null(projections)) {
return(NULL)
}
if (.schnappsEnv$DEBUGSAVE) {
save(file = normalizePath("~/SCHNAPPsDebug/GS_DF_button.RData"), list = c(ls()))
}
# cp = load(file = "~/SCHNAPPsDebug/GS_DF_button.RData")
dubs = tryCatch({
find_doublets_m(scEx, dims=1:dims, n_recovered=nRecover, pK=pK, pN=pN)
},
error = function(e) {
cat(file = stderr(), paste("\n\n!!!Error during detach process:", e, "\n\nDo you need to increase the memory?\n\n"))
if (!is.null(getDefaultReactiveDomain())) {
showNotification("DE_scaterPNG ERROR", id = "DE_scaterPNG_Error", duration = NULL, type = "error")
}
return(NULL)
}
)
if (ncol(newPrjs) == 0) {
newPrjs = data.frame(row.names = acn)
}
newPrjs[,colnames(dubs)] = 0
newPrjs[rownames(dubs),colnames(dubs)] = dubs
sessionProjections$prjs <- newPrjs
.schnappsEnv[["GS_DF_plot-dimension_x"]] <- "barcode"
.schnappsEnv$defaultValues[["GS_DF_plot-dimension_x"]] <- "barcode"
.schnappsEnv[["GS_DF_plot-dimension_y"]] <- colnames(dubs)[1]
.schnappsEnv$defaultValues[["GS_DF_plot-dimension_y"]] <- colnames(dubs)[1]
.schnappsEnv[["GS_DF_plot-dimension_col"]] <- colnames(dubs)[2]
.schnappsEnv$defaultValues[["GS_DF_plot-dimension_col"]] <- colnames(dubs)[2]
updateSelectizeInput(session = session, inputId = "GS_DF_plot-dimension_x", selected = "barcode")
updateSelectizeInput(session = session, inputId = "GS_DF_plot-dimension_y", selected = colnames(dubs)[1])
updateSelectizeInput(session = session, inputId = "GS_DF_plot-dimension_col", selected = colnames(dubs)[2])
})
# gc_DF_2D <-
callModule(
clusterServer,
"GS_DF_plot",
projections # ,
# reactive(input$coE_gene_id_sch)
)
}
C3BI-pasteur-fr/UTechSCB-SCHNAPPs documentation built on Sept. 8, 2024, 12:44 a.m.
R Package Documentation
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Note that we can't provide technical support on individual packages. You should contact the package authors for that.
# generalQC outputs.R
# source("moduleServer.R", local = TRUE)
# source("reactives.R", local = TRUE)
# TODO: verify that this anything and then integrate in DUMMY
myZippedReportFiles <- c("gqcProjections.csv")
require(stringr)
.schnappsEnv$gQC_X1 <- "tsne1"
.schnappsEnv$gQC_X2 <- "tsne2"
.schnappsEnv$gQC_X3 <- "tsne3"
.schnappsEnv$gQC_col <- "sampleNames"
observe(label = "ob2", {
if (DEBUG) cat(file = stderr(), "observe: gQC_dim3D_x\n")
.schnappsEnv$gQC_X1 <- input$gQC_dim3D_x
})
observe(label = "ob3", {
if (DEBUG) cat(file = stderr(), "observe: gQC_dim3D_y\n")
.schnappsEnv$gQC_X2 <- input$gQC_dim3D_y
})
observe(label = "ob4", {
if (DEBUG) cat(file = stderr(), "observe: gQC_dim3D_z\n")
.schnappsEnv$gQC_X3 <- input$gQC_dim3D_z
})
observe(label = "ob5", {
if (DEBUG) cat(file = stderr(), "observe: gQC_col3D\n")
.schnappsEnv$gQC_col <- input$gQC_col3D
})
observe(label = "obs_gQC_tsnePerplexity", x = {
.schnappsEnv$defaultValues[["gQC_tsnePerplexity"]] = input$gQC_tsnePerplexity
})
observe(label = "obs_gQC_tsneSeed", x = {
.schnappsEnv$defaultValues[["gQC_tsneSeed"]] = input$gQC_tsneSeed
})
observe(label = "obs_gQC_tsneTheta", x = {
.schnappsEnv$defaultValues[["gQC_tsneTheta"]] = input$gQC_tsneTheta
})
observe(label = "obs_gQC_tsneDim", x = {
.schnappsEnv$defaultValues[["gQC_tsneDim"]] = input$gQC_tsneDim
})
observe(label = "obs_gQC_dim3D_x", x = {
.schnappsEnv$defaultValues[["gQC_dim3D_x"]] = input$gQC_dim3D_x
})
observe(label = "obs_gQC_dim3D_y", x = {
.schnappsEnv$defaultValues[["gQC_dim3D_y"]] = input$gQC_dim3D_y
})
observe(label = "obs_gQC_dim3D_z", x = {
.schnappsEnv$defaultValues[["gQC_dim3D_z"]] = input$gQC_dim3D_z
})
observe(label = "obs_gQC_col3D", x = {
.schnappsEnv$defaultValues[["gQC_col3D"]] = input$gQC_col3D
})
observe(label = "obs_gQC_um_spread", x = {
.schnappsEnv$defaultValues[["gQC_um_spread"]] = input$gQC_um_spread
})
observe(label = "obs_gQC_um_n_components", x = {
.schnappsEnv$defaultValues[["gQC_um_n_components"]] = input$gQC_um_n_components
})
observe(label = "obs_gQC_um_n_neighbors", x = {
.schnappsEnv$defaultValues[["gQC_um_n_neighbors"]] = input$gQC_um_n_neighbors
})
observe(label = "obs_gQC_um_init", x = {
.schnappsEnv$defaultValues[["gQC_um_init"]] = input$gQC_um_init
})
observe(label = "obs_gQC_um_negative_sample_rate", x = {
.schnappsEnv$defaultValues[["gQC_um_negative_sample_rate"]] = input$gQC_um_negative_sample_rate
})
observe(label = "obs_gQC_um_randSeed", x = {
.schnappsEnv$defaultValues[["gQC_um_randSeed"]] = input$gQC_um_randSeed
})
observe(label = "obs_gQC_um_local_connectivity", x = {
.schnappsEnv$defaultValues[["gQC_um_local_connectivity"]] = input$gQC_um_local_connectivity
})
observe(label ="obs_gQC_um_bandwidth", x = {
.schnappsEnv$defaultValues[["gQC_um_bandwidth"]] = input$gQC_um_bandwidth
})
observe(label ="obs_gQC_um_n_epochs", x = {
.schnappsEnv$defaultValues[["gQC_um_n_epochs"]] = input$gQC_um_n_epochs
})
observe(label ="obs_gQC_um_set_op_mix_ratio", x = {
.schnappsEnv$defaultValues[["gQC_um_set_op_mix_ratio"]] = input$gQC_um_set_op_mix_ratio
})
observe(label ="obs_gQC_um_metric", x = {
.schnappsEnv$defaultValues[["gQC_um_metric"]] = input$gQC_um_metric
})
observe(label ="obs_gQC_um_min_dist", x = {
.schnappsEnv$defaultValues[["gQC_um_min_dist"]] = input$gQC_um_min_dist
})
observe(label ="obs_gQC_binSize", x = {
.schnappsEnv$defaultValues[["gQC_binSize"]] = input$gQC_binSize
})
# gQC_update3DInput ----
#' gQC_update3DInput
#' update axes for tsne display
gQC_update3DInput <- observeEvent(projections(),{
if (DEBUG) cat(file = stderr(), "gQC_update3DInput started.\n")
start.time <- base::Sys.time()
on.exit({
printTimeEnd(start.time, "gQC_update3DInput")
if (!is.null(getDefaultReactiveDomain())) {
removeNotification(id = "gQC_update3DInput")
}
})
if (!is.null(getDefaultReactiveDomain())) {
showNotification("gQC_update3DInput", id = "gQC_update3DInput", duration = NULL)
}
projections <- projections()
# Can use character(0) to remove all choices
if (is.null(projections)) {
return(NULL)
}
# choices = colnames(projections)[unlist(lapply(colnames(projections), function(x) !is.factor(projections[,x])))]
choices <- colnames(projections)
# Can also set the label and select items
updateSelectInput(session, "gQC_dim3D_x",
choices = choices,
selected = .schnappsEnv$gQC_X1
)
updateSelectInput(session, "gQC_dim3D_y",
choices = choices,
selected = .schnappsEnv$gQC_X2
)
updateSelectInput(session, "gQC_dim3D_z",
choices = choices,
selected = .schnappsEnv$gQC_X3
)
updateSelectInput(session, "gQC_col3D",
choices = colnames(projections),
selected = .schnappsEnv$gQC_col
)
})
# observer of UMAP button ----
observe(label = "ob_UMAPParams", {
# save(file = "~/SCHNAPPsDebug/ob_UMAPParams.RData", list = c(ls(), ".schnappsEnv"))
# load("~/SCHNAPPsDebug/updateButtonColor.RData")
deepDebug()
if (DEBUG) cat(file = stderr(), "observe umapVars\n")
input$activateUMAP
setRedGreenButtonCurrent(
vars = list(
c("gQC_um_randSeed", input$gQC_um_randSeed),
c("gQC_um_n_neighbors", input$gQC_um_n_neighbors),
c("gQC_um_n_components", input$gQC_um_n_components),
c("gQC_um_n_epochs", input$gQC_um_n_epochs),
# c("um_alpha", input$um_alpha),
c("gQC_um_init", input$gQC_um_init),
c("gQC_um_min_dist", input$gQC_um_min_dist),
c("gQC_um_set_op_mix_ratio", input$gQC_um_set_op_mix_ratio),
c("gQC_um_local_connectivity", input$gQC_um_local_connectivity),
c("gQC_um_bandwidth", input$gQC_um_bandwidth),
c("um_gamma", input$um_gamma),
c("gQC_um_negative_sample_rate", input$gQC_um_negative_sample_rate),
c("gQC_um_metric", input$gQC_um_metric),
c("gQC_um_spread", input$gQC_um_spread)
)
)
updateButtonColor(buttonName = "activateUMAP", parameters = c(
"gQC_um_randSeed", "gQC_um_n_neighbors", "gQC_um_n_components", "gQC_um_n_epochs",
"gQC_um_init", "gQC_um_min_dist", "gQC_um_set_op_mix_ratio",
"gQC_um_local_connectivity", "gQC_um_bandwidth", "um_gamma",
"gQC_um_negative_sample_rate", "gQC_um_metric", "gQC_um_spread"
))
})
## observe coE_updateInputXviolinPlot ----
observe({
if (DEBUG) cat(file = stderr(), "coE_updateInputXviolinPlot started.\n")
start.time <- base::Sys.time()
on.exit({
printTimeEnd(start.time, "coE_updateInputXviolinPlot")
if (!is.null(getDefaultReactiveDomain())) {
removeNotification(id = "coE_updateInputXviolinPlot")
}
})
if (!is.null(getDefaultReactiveDomain())) {
showNotification("coE_updateInputXviolinPlot", id = "coE_updateInputXviolinPlot", duration = NULL)
}
tsneData <- projections()
projFactors <- projFactors()
# Can use character(0) to remove all choices
if (is.null(tsneData)) {
return(NULL)
}
updateSelectInput(
session,
"coE_dimension_xVioiGrp",
choices = projFactors,
selected = .schnappsEnv$coE_dimension_xVioiGrp
)
updateSelectInput(
session,
"coE_scranFactor",
choices = projFactors
)
updateSelectInput(
session,
"coE_dimension_xVioiGrp2",
choices = projFactors,
selected = .schnappsEnv$coE_dimension_xVioiGrp2
)
})
# observe: cellNameTable_rows_selected ----
observe(label = "ob_tsneParams", {
deepDebug()
if (DEBUG) cat(file = stderr(), "observe tsneVars\n")
out <- tsne()
if (is.null(out)) {
.schnappsEnv$calculated_gQC_tsneDim <- "NA"
}
input$updatetsneParameters
setRedGreenButtonCurrent(
vars = list(
c("gQC_tsneDim", input$gQC_tsneDim),
c("gQC_tsnePerplexity", input$gQC_tsnePerplexity),
c("gQC_tsneTheta", input$gQC_tsneTheta),
c("gQC_tsneSeed", input$gQC_tsneSeed)
)
)
updateButtonColor(buttonName = "updatetsneParameters", parameters = c(
"gQC_tsneDim", "gQC_tsnePerplexity",
"gQC_tsneTheta", "gQC_tsneSeed"
))
})
# gQC_tsne_main ----
output$gQC_tsne_main <- plotly::renderPlotly({
if (DEBUG) cat(file = stderr(), "gQC_tsne_main started.\n")
start.time <- base::Sys.time()
on.exit({
printTimeEnd(start.time, "gQC_tsne_main")
if (!is.null(getDefaultReactiveDomain())) {
removeNotification(id = "gQC_tsne_main")
}
})
if (!is.null(getDefaultReactiveDomain())) {
showNotification("gQC_tsne_main", id = "gQC_tsne_main", duration = NULL)
}
# upI <- gQC_update3DInput()
projections <- projections()
dimX <- input$gQC_dim3D_x
dimY <- input$gQC_dim3D_y
dimZ <- input$gQC_dim3D_z
dimCol <- input$gQC_col3D
# scols <- projectionColors$sampleNames
# ccols <- clusterCols$colPal
pc = projectionColors %>% reactiveValuesToList()
if (is.null(projections)) {
if (DEBUG) cat(file = stderr(), "output$gQC_tsne_main:NULL\n")
return(NULL)
}
if (.schnappsEnv$DEBUGSAVE) {
save(file = normalizePath("~/SCHNAPPsDebug/gQC_tsne_main.RData"), list = c(ls()))
}
# cp =load(file="~/SCHNAPPsDebug/gQC_tsne_main.RData")
retVal <- tsnePlot(projections, dimX, dimY, dimZ, dimCol, projColors = pc)
exportTestValues(tsnePlot = {
str(retVal)
})
layout(retVal)
})
# gQC_umap_main 2D plot ----
callModule(
clusterServer,
"gQC_umap_main",
projections
)
# gQC_projectionTableMod ----
callModule(
tableSelectionServer,
"gQC_projectionTableMod",
projectionTable, caption = "Table with projections"
)
# gQC_projectionCombTableMod ----
callModule(
tableSelectionServer,
"gQC_projCombTableMod",
projectionTable, caption = "Tables with projections"
)
# gQC_plotUmiHist ----
output$gQC_plotUmiHist <- plotly::renderPlotly({
if (DEBUG) cat(file = stderr(), "gQC_plotUmiHist started.\n")
start.time <- base::Sys.time()
on.exit({
printTimeEnd(start.time, "gQC_plotUmiHist")
if (!is.null(getDefaultReactiveDomain())) {
removeNotification(id = "gQC_plotUmiHist")
}
})
if (!is.null(getDefaultReactiveDomain())) {
showNotification("gQC_plotUmiHist", id = "gQC_plotUmiHist", duration = NULL)
}
scEx <- scEx()
pc = projectionColors %>% reactiveValuesToList()
scols <- projectionColors$sampleNames
binSize <- input$gQC_binSize
if (is.null(scEx)) {
return(NULL)
}
if (.schnappsEnv$DEBUGSAVE) {
save(file = normalizePath("~/SCHNAPPsDebug/gQC_plotUmiHist.RData"), list = c(ls()))
}
# cp = load(file = "~/SCHNAPPsDebug/gQC_plotUmiHist.RData")
dat <- data.frame(counts = Matrix::colSums(assays(scEx)[["counts"]]))
dat$sample <- colData(scEx)$sampleNames
fig <- plotly::plot_ly(alpha = 1,
nbinsx = binSize)
# dat[dat$sample == levels(colData(scEx)$sampleNames)[[1]],],
# x = ~counts,
# # y = ~counts,
# type="histogram")
lev = levels(colData(scEx)$sampleNames)
for (idx in seq_along(lev)) {
fig <- fig %>% add_trace(
type = 'histogram', color = I(scols[idx]), name = lev[idx],
x = dat[dat$sample == levels(colData(scEx)$sampleNames)[[idx]],"counts"]
)
}
fig <- fig %>% layout(
barmode="stack",
bargap=0.1,
title = "Histogram of UMIs",
yaxis = list(title = "Number of cells"),
xaxis = list(title = "UMI count"))
fig
# marker = list(color = scols))
# retVal <- ggplot(data = dat, aes(counts, fill = sample)) +
# geom_histogram(bins = 50) +
# labs(title = "Histogram for raw counts", x = "count", y = "Frequency") +
# scale_fill_manual(values = scols, aesthetics = "fill")
#
.schnappsEnv[["gQC_plotUmiHist"]] <- fig
return(fig)
})
# gQC_plotSampleHist -----
output$gQC_plotSampleHist <- plotly::renderPlotly({
if (DEBUG) cat(file = stderr(), "gQC_plotSampleHist started.\n")
start.time <- base::Sys.time()
on.exit({
printTimeEnd(start.time, "gQC_plotSampleHist")
if (!is.null(getDefaultReactiveDomain())) {
removeNotification(id = "gQC_plotSampleHist")
}
})
if (!is.null(getDefaultReactiveDomain())) {
showNotification("gQC_plotSampleHist", id = "gQC_plotSampleHist", duration = NULL)
}
sampleInf <- sampleInfo()
scols <- projectionColors$sampleNames
if (is.null(sampleInf)) {
return(NULL)
}
if (.schnappsEnv$DEBUGSAVE) {
save(file = normalizePath("~/SCHNAPPsDebug/sampleHist.RData"), list = c(ls()))
}
# cp = load(file = "~/SCHNAPPsDebug/sampleHist.RData")
retVal <- gQC_sampleHistFunc(sampleInf, scols)
.schnappsEnv[["gQC_plotSampleHist"]] <- retVal
return(retVal)
})
output$gQC_variancePCA <- renderPlot({
if (DEBUG) cat(file = stderr(), "gQC_variancePCA started.\n")
start.time <- base::Sys.time()
on.exit({
printTimeEnd(start.time, "gQC_variancePCA")
if (!is.null(getDefaultReactiveDomain())) {
removeNotification(id = "gQC_variancePCA")
}
})
if (!is.null(getDefaultReactiveDomain())) {
showNotification("gQC_variancePCA", id = "gQC_variancePCA", duration = NULL)
}
pca <- pcaReact()
if (is.null(pca)) {
return(NULL)
}
if (.schnappsEnv$DEBUGSAVE) {
save(file = normalizePath("~/SCHNAPPsDebug/gQC_variancePCA.RData"), list = c(ls()))
}
# load(file = "~/SCHNAPPsDebug/gQC_variancePCA.RData")
# h2("Variances of PCs")
df <- data.frame(var = pca$var_pcs, pc = 1:length(pca$var_pcs))
retVal <- plotHistVarPC(df, pc, var)
af = plotHistVarPC
# remove env because it is too big
environment(af) = new.env(parent = emptyenv())
.schnappsEnv[["gQC_variancePCA"]] <- list(plotFunc = af,
df, pc, var)
# .schnappsEnv[["gQC_variancePCA"]] <- retVal
return(retVal)
# barplot(pca$var_pcs, main = "Variance captured by first PCs")
})
# gene set related -----
## modify a gene set ----
observeEvent(input$geneSetModifyButton,{
if (DEBUG) cat(file = stderr(), "geneSetModifyButton\n")
start.time <- base::Sys.time()
on.exit({
printTimeEnd(start.time, "geneSetModifyButton")
if (!is.null(getDefaultReactiveDomain())) {
removeNotification(id = "geneSetModifyButton")
}
})
# show in the app that this is running
if (!is.null(getDefaultReactiveDomain())) {
showNotification("geneSetModifyButton", id = "geneSetModifyButton", duration = NULL)
}
# inputGS <- input$gQC_geneSetModifyInput
newName = make.names(input$gQC_geneSetModifynName)
newGenes = input$gQC_geneSetModifyGenes
desc = input$gQC_geneSetModifynDesc
gd = gmtData()
userData = gmtUserData()
scEx = scEx()
featureData <- rowData(scEx)
# browser()
# deepDebug()
if (.schnappsEnv$DEBUGSAVE) {
save(file = normalizePath("~/SCHNAPPsDebug/geneSetModifyButton.RData"), list = c(ls()))
}
# cp = load(file="~/SCHNAPPsDebug/geneSetModifyButton.RData")
if (newGenes == "" | newName == "X" | is.null(scEx)) {
return(NULL)
}
# li = new gene list
li <- geneName2Index(g_id = newGenes, featureData = featureData)
if(is.null(li)) {
cat(file = stderr(), "!!!!geneSetModifyButton: no genes found\n")
return(NULL)
}
if(newName %in% names(gd)) {
cat(file = stderr(), "!!!!geneSetModifyButton: gene set already set.\n")
return(NULL)
}
li = list(list(genes = featureData[li,"symbol"], desc = desc, name = newName))
names(li) = newName
# append to global list
if(is.null(userData)){
gmtUserData(li)
} else {
gmtUserData(append(userData, li))
}
.schnappsEnv$defaultValues["gQC_geneSetModifyInput"] = newName
updateSelectizeInput(
session = session,
inputId = "gQC_geneSetModifyInput",
# choices = ,
selected = newName
)
})
# GSEA ----
output$gQC_geneSetsearchOutput = renderText({
if (DEBUG) cat(file = stderr(), "geneSetsearchOutput\n")
start.time <- base::Sys.time()
on.exit({
printTimeEnd(start.time, "geneSetsearchOutput")
if (!is.null(getDefaultReactiveDomain())) {
removeNotification(id = "geneSetsearchOutput")
}
})
# show in the app that this is running
if (!is.null(getDefaultReactiveDomain())) {
showNotification("geneSetsearchOutput", id = "geneSetsearchOutput", duration = NULL)
}
# inputGS <- input$gQC_geneSetModifyInput
genes = input$gQC_genesets_search
maxItems = isolate(input$gQC_genesets_maxPrint)
gd = gmtData()
userData = gmtUserData()
scEx = scEx()
featureData <- rowData(scEx)
# browser()
# deepDebug()
if (.schnappsEnv$DEBUGSAVE) {
save(file = normalizePath("~/SCHNAPPsDebug/geneSetsearchOutput.RData"), list = c(ls()))
}
# cp = load(file="~/SCHNAPPsDebug/geneSetsearchOutput.RData")
if ( is.null(scEx)) {
return(NULL)
}
# li = new gene list
li <- geneName2Index(g_id = genes, featureData = featureData)
if(is.null(li)) {
cat(file = stderr(), "!!!!geneSetsearchOutput: no genes found\n")
return(NULL)
}
# parallel bplapply on gmtData -- fast enough
counts = bplapply(gd, FUN=function(x)sum(li %in% x$genes))
counts = counts[which(counts>0)]
outStr = ""
countNames = counts %>% unlist() %>% sort(decreasing = T) %>% names()
maxItems = min(maxItems, length(countNames))
for (name in countNames[1:maxItems]){
outStr = paste(outStr, name, "found:", counts[[name]], " of ", length(gd[[name]]$genes),"\n",
gd[[name]]$desc, "\n",
paste(li[which(li %in% gd[[name]]$genes)], collapse=", "), "\n",
paste(gd[[name]]$genes, collapse=", "), "\n\n")
}
outStr
})
# %>% bindCache(input$gQC_genesets_search, gmtData())
# gQC_renameGenes ----
output$gQC_renameGenes <- renderText({
if (DEBUG) cat(file = stderr(), "gQC_renameGenes\n")
start.time <- base::Sys.time()
on.exit({
printTimeEnd(start.time, "gQC_renameGenes")
if (!is.null(getDefaultReactiveDomain())) {
removeNotification(id = "gQC_renameGenes")
}
})
# show in the app that this is running
if (!is.null(getDefaultReactiveDomain())) {
showNotification("gQC_renameGenes", id = "gQC_renameGenes", duration = NULL)
}
gd = gmtData()
inputGS <- input$oldGS
req(gd)
if (inputGS == "") {
return(NULL)
}
# deepDebug()
if (.schnappsEnv$DEBUGSAVE) {
save(file = normalizePath("~/SCHNAPPsDebug/gQC_renameGenes.RData"), list = c(ls()))
}
# cp = load(file="~/SCHNAPPsDebug/gQC_renameGenes.RData")
if(inputGS %in% names(gd)) {
retVal = paste(gd[[inputGS]]$genes, collapse = ", ")
} else {
retVal = ""
}
return(retVal)
})
output$gQC_geneSetModifyInputGL <- renderText({
if (DEBUG) cat(file = stderr(), "gQC_geneSetModifyInputGL\n")
start.time <- base::Sys.time()
on.exit({
printTimeEnd(start.time, "gQC_geneSetModifyInputGL")
if (!is.null(getDefaultReactiveDomain())) {
removeNotification(id = "gQC_geneSetModifyInputGL")
}
})
# show in the app that this is running
if (!is.null(getDefaultReactiveDomain())) {
showNotification("gQC_geneSetModifyInputGL", id = "gQC_geneSetModifyInputGL", duration = NULL)
}
gd = gmtData()
inputGS <- input$gQC_geneSetModifyInput
req(gd)
if (inputGS == "") {
return(NULL)
}
# deepDebug()
if (.schnappsEnv$DEBUGSAVE) {
save(file = normalizePath("~/SCHNAPPsDebug/gQC_geneSetModifyInput.RData"), list = c(ls()))
}
# cp = load(file="~/SCHNAPPsDebug/gQC_geneSetModifyInput.RData")
if(inputGS %in% names(gd)) {
retVal = paste(gd[[inputGS]]$genes, collapse = ", ")
} else {
retVal = ""
}
return(retVal)
})
observe({
gd = gmtData()
updateSelectizeInput(session, inputId = "oldGS",choices = names(gd), server = TRUE)
updateSelectizeInput(session, inputId = "gQC_geneSetModifyInput",
choices = names(gd),
selected = defaultValue("gQC_geneSetModifyInput", "dummy"), server = TRUE)
})
observeEvent(input$updateGSButton,{
userData = gmtUserData()
gd = gmtData()
name = input$oldGS
newName = input$newGS
if(!name %in% names(gd)) return(NULL)
li = gd[name]
names(li) = newName
gmtUserData(append(userData, li))
if(.schnappsEnv$DEBUGSAVE){
save(file = normalizePath("~/SCHNAPPsDebug/updateGSButton.RData"), list = c(ls()))
}
# cp =load(file='~/SCHNAPPsDebug/updateGSButton.RData')
})
# rename projections observers ----
observeEvent(
label = "ob30",
eventExpr = input$updatePrjsButton,
handlerExpr = {
deepDebug()
if (DEBUG) cat(file = stderr(), "updatePrjsButton\n")
oldPrj <- input$oldPrj
newPrj <- input$newPrj
projections <- projections()
acn = allCellNames()
newPrjs <- projectionsTable$newProjections
if (is.null(projections)) {
return(NULL)
}
if (.schnappsEnv$DEBUGSAVE) {
save(file = normalizePath("~/SCHNAPPsDebug/updatePrjsButton.RData"),
list = c("normaliztionParameters", ls())
)
}
# cp = load(file="~/SCHNAPPsDebug/updatePrjsButton.RData")
# deepDebug()
if (newPrj %in% colnames(projections)) {
showNotification(
"New column name already used",
type = "error",
duration = NULL
)
return(NULL)
}
if (ncol(newPrjs) == 0) {
newPrjs = data.frame(row.names = acn)
newPrjs[,newPrj] = NA
newPrjs[rownames(projections),newPrj] <- projections[, oldPrj, drop = FALSE]
} else {
# newPrjs <- cbind(newPrjs[rownames(projections), , drop = FALSE], projections[, oldPrj, drop = FALSE])
# deepDebug()
newPrjs <- dplyr::full_join(
tibble::rownames_to_column(newPrjs),
tibble::rownames_to_column(projections[, oldPrj, drop = FALSE]),
by='rowname')
rownames(newPrjs) = newPrjs[,1]
newPrjs = newPrjs[,-1]
}
colnames(newPrjs)[ncol(newPrjs)] <- newPrj
if(is.factor(projections[, oldPrj])) newPrjs[,newPrj] = as.factor(newPrjs[,newPrj])
projectionsTable$newProjections <- newPrjs
}
)
# # rename projections
# observe(label = "ob27", {
# projections <- projections()
#
# })
observe(label = "ob28", {
deepDebug()
input$newPrj
updateTextInput(session, "newPrj", value = make.names(input$newPrj, unique = TRUE))
})
observeEvent(
label = "ob29",
eventExpr = input$delPrjsButton,
handlerExpr = {
deepDebug()
if (DEBUG) cat(file = stderr(), "updatePrjsButton\n")
newPrjs <- projectionsTable$newProjections
delPrj <- input$delPrj
if (is.null(projections)) {
return(NULL)
}
if (!delPrj %in% colnames(newPrjs)) {
return(NULL)
}
# deepDebug()
if (.schnappsEnv$DEBUGSAVE) {
save(file = normalizePath("~/SCHNAPPsDebug/delPrjsButton.RData"),
list = c("normaliztionParameters", ls())
)
}
# load(file="~/SCHNAPPsDebug/delPrjsButton.RData")
projectionsTable$newProjections <- newPrjs[, -which(colnames(newPrjs) == delPrj), drop = FALSE]
}
)
# combine projections observers ----
observeEvent(
label = "gQC_updateCombPrjsButton",
eventExpr = input$gQC_updateCombPrjsButton,
handlerExpr = {
if (DEBUG) cat(file = stderr(), "gQC_updateCombPrjsButton\n")
prj1 <- input$gQC_combPrj1
prj2 <- input$gQC_combPrj2
newPrj <- make.names(input$gQC_newCombPrj)
projections <- projections()
newPrjs <- projectionsTable$newProjections
acn = allCellNames()
if (is.null(projections)) {
return(NULL)
}
if (!all(c(prj1, prj2) %in% colnames(projections))) {
return(NULL)
}
if (.schnappsEnv$DEBUGSAVE) {
save(file = normalizePath("~/SCHNAPPsDebug/gQC_updateCombPrjsButton.RData"),
list = c("normaliztionParameters", ls())
)
}
# cp= load(file="~/SCHNAPPsDebug/gQC_updateCombPrjsButton.RData")
if (newPrj %in% colnames(projections)) {
showNotification(
"New column name already used",
type = "error",
duration = NULL
)
return(NULL)
}
# deepDebug()
combProjections = data.frame(row.names = rownames(projections),
newPrj= paste(projections[,prj1], projections[,prj2], sep = " - ") %>% as.factor())
if (length(levels(combProjections[,1])) > 100) {
out = showModal(verifyLevelModal(NLevel = length(levels(combProjections[,1]))))
if (DEBUG) cat(file = stderr(), paste("gQC_updateCombPrjsButton modal out:", out, "\n"))
# deepDebug()
}
if (ncol(newPrjs) == 0) {
newPrjs <- data.frame(row.names = acn)
newPrjs[rownames(combProjections),newPrj] = combProjections[,1]
# rownames(newPrjs) = rownames(projections)
} else {
# newPrjs <- cbind(newPrjs[rownames(projections), , drop = FALSE], combProjections)
# deepDebug()
newPrjs <- dplyr::left_join(
tibble::rownames_to_column(newPrjs),
tibble::rownames_to_column(combProjections),
by='rowname')
rownames(newPrjs) = newPrjs[,1]
newPrjs = newPrjs[,-1]
}
colnames(newPrjs)[ncol(newPrjs)] <- newPrj
projectionsTable$newProjections <- newPrjs
}
)
# output$gQC_orgLevels ----
output$gQC_orgLevels = renderText({
rnProj = input$gQC_rnProj
projections = projections()
shiny::req(rnProj)
shiny::req(projections)
if (! rnProj %in% colnames(projections)) return(NULL)
# deepDebug()
paste(levels(factor(projections[,rnProj])), collapse = ", ")
})
# rename projection levels ----
verifyLevelModal <- function(NLevel, failed = FALSE) {
modalDialog(
span(paste(
"There are ", NLevel, "new levels, are you sure you want to do this?\n")
)
,
footer = tagList(
modalButton("Cancel"),
actionButton("commentok", "OK")
)
)
}
# gQC_rearrange levels ----
observeEvent(eventExpr = input$gQC_raProj,
label = "raLevBtn",
handlerExpr = {
deepDebug()
projections <- projections()
projFactors <- projFactors()
if(is.null(projections)) return()
if(is.null(projFactors)) return()
if(input$gQC_raProj %in% projFactors){
projLevels = levels(projections[,input$gQC_raProj])
updateOrderInput(
session,
'gQC_newRaLev',
items = projLevels
)
}else{
updateOrderInput(
session,
'gQC_newRaLev',
items = "not yet"
)
}
}
)
observeEvent(eventExpr = input$gQC_rearrangeLevButton,
label = "raLevBtn",
handlerExpr = {
deepDebug()
newProjName = make.names(input$gQC_newRaPrj)
newLevelOrder = input$gQC_newRaLev
projections = projections()
raProj = input$gQC_raProj
acn = allCellNames()
newPrjs <- projectionsTable$newProjections
if (is.null(projections)) {
return(NULL)
}
if (.schnappsEnv$DEBUGSAVE) {
save(file = normalizePath("~/SCHNAPPsDebug/gQC_rearrangeButton.RData"),
list = c("normaliztionParameters", ls())
)
}
# cp= load(file="~/SCHNAPPsDebug/gQC_rearrangeButton.RData")
orgLevelNames = levels(factor(projections[,raProj]))
newLbVec = stringr::str_trim(str_split(newLevelOrder, ","))
# names(newLbVec) = orgLevelNames
# deepDebug()
# sampe projections as displayed, i.e. only those available for the cells
# otherwise the diplay (output$gQC_orgLevels) has to be changed as well
projections[,raProj] = factor(projections[,raProj])
if(is.null(
tryCatch({
if (ncol(newPrjs) == 0) {
newPrjs = data.frame(row.names = acn)
newPrjs[,newProjName] = "NA"
# drop = TRUE: we re interested in the vector not the data frame
newPrjs[rownames(projections),newProjName] <- as.character(projections[, raProj, drop = TRUE])
} else {
# deepDebug()
newPrjs <- dplyr::full_join(
tibble::rownames_to_column(newPrjs),
tibble::rownames_to_column(projections[, raProj, drop = FALSE]),
by='rowname')
rownames(newPrjs) = newPrjs[,1]
newPrjs = newPrjs[,-1]
# newPrjs <- cbind(newPrjs[rownames(projections), , drop = FALSE], projections[,raProj])
}
newPrjs[,ncol(newPrjs)] = factor(newPrjs[,ncol(newPrjs)], levels = newLbVec)
# # in case there was NA introduced by hidden cells
# if ("NA" %in% levels(newPrjs[,ncol(newPrjs)]) & !"NA" %in% newLevelOrder ){
# newLevelOrder = c(newLevelOrder, "NA")
# }
# if (!length(levels(newPrjs[,ncol(newPrjs)])) == length(newLevelOrder) ){
# cat(file = stderr(), paste("number of levels not correct\n\nold levels:\n"))
# cat(file = stderr(), levels(newPrjs[,ncol(newPrjs)]))
# cat(file = stderr(), paste("\n\n\nnew levels:\n"))
# cat(file = stderr(), newLevelOrder)
# cat(file = stderr(), paste("\n"))
# showNotification("number of levels not correct. See console", id = "renameProbl", duration = NULL, type = "error")
# return(NULL)
# }
# newPrjs[,ncol(newPrjs)] = factor(newPrjs[,ncol(newPrjs)], levels = newLbVec)
}, error=function(w){
# deepDebug()
cat(file = stderr(), paste("something went wrong during releveling", w,"\n"))
showNotification("problem with names", id = "renameProbl", duration = NULL, type = "error")
return(NULL)
}))) return(NULL)
# newProjName = make.unique(c(colnames(projections),newProjName))[length(c(colnames(projections),newProjName))]
# updateTextInput(session, "gQC_newRnPrj", value = newProjName)
colnames(newPrjs)[ncol(newPrjs)] <- newProjName
projectionsTable$newProjections <- newPrjs
})
# gQC_renameLevButton ----
observeEvent(eventExpr = input$gQC_renameLevButton,
label = "rnLevBtn",
handlerExpr = {
deepDebug()
newLables = input$gQC_renameLev
rnProj = input$gQC_rnProj
newProjName = make.names(input$gQC_newRnPrj)
projections = projections()
acn = allCellNames()
newPrjs <- projectionsTable$newProjections
if (is.null(projections)) {
return(NULL)
}
orgLevelNames = levels(factor(projections[,rnProj]))
newLbVec = stringr::str_trim(str_split(newLables, ",")[[1]]) %>% make.names()
names(newLbVec) = orgLevelNames
if (.schnappsEnv$DEBUGSAVE) {
# browser()
save(file = normalizePath("~/SCHNAPPsDebug/gQC_renameLevButton.RData"),
list = c("normaliztionParameters", ls())
)
}
# cp= load(file="~/SCHNAPPsDebug/gQC_renameLevButton.RData")
# deepDebug()
# sampe projections as displayed, i.e. only those available for the cells
# otherwise the display (output$gQC_orgLevels) has to be changed as well
proj2Add = projections[,rnProj,drop=FALSE]
proj2Add[,rnProj] = factor(proj2Add[,rnProj])
if(is.null(
tryCatch({
# copy original data
# This can be the first newPrjs
if (ncol(newPrjs) == 0) {
newPrjs = data.frame(row.names = acn)
newPrjs[,newProjName] = "NA"
# drop = TRUE: we re interested in the vector not the data frame
newPrjs[rownames(projections),newProjName] <- as.character(projections[, rnProj, drop = TRUE])
} else {
# deepDebug()
newPrjs <- dplyr::full_join(
tibble::rownames_to_column(newPrjs),
tibble::rownames_to_column(projections[, rnProj, drop = FALSE]),
by='rowname')
rownames(newPrjs) = newPrjs[,1]
newPrjs = newPrjs[,-1]
# newPrjs <- cbind(newPrjs[rownames(projections), , drop = FALSE], projections[,rnProj])
}
# ensure that this is a factor
newPrjs[,ncol(newPrjs)] = as.factor(newPrjs[,ncol(newPrjs)])
if ("NA" %in% levels(newPrjs[,ncol(newPrjs)]) & !"NA" %in% stringr::str_trim(newLbVec) ){
newLevelNames = levels(newPrjs[,ncol(newPrjs)])
naPos = which ("NA" == newLevelNames)
newLbVec = newLbVec[newLevelNames]
newLbVec[which(is.na(newLbVec))] = "NA"
}
# if (!length(levels(newPrjs[,ncol(newPrjs)])) == length(stringr::str_trim(newLbVec)) ){
# cat(file = stderr(), paste("number of levels not correct\n\nold levels:\n"))
# cat(file = stderr(), levels(newPrjs[,ncol(newPrjs)]))
# cat(file = stderr(), paste("\n\n\nnew levels:\n"))
# cat(file = stderr(), stringr::str_trim(newLbVec))
# cat(file = stderr(), paste("\n"))
# showNotification("number of levels not correct. See console", id = "renameProbl", duration = NULL, type = "error")
# return(NULL)
# }
oldLevels = levels(newPrjs[,ncol(newPrjs)])
names(oldLevels) = oldLevels
oldLevels[names(newLbVec)] = stringr::str_trim(newLbVec)
levels(newPrjs[,ncol(newPrjs)]) = oldLevels
}, error=function(w){
# deepDebug()
cat(file = stderr(), paste("something went wrong during releveling", w,"\n"))
showNotification("problem with names", id = "renameProbl", duration = NULL, type = "error")
return(NULL)
}))) return(NULL)
newProjName = make.unique(c(colnames(projections),newProjName)) %>% tail(n=1)
colnames(newPrjs)[ncol(newPrjs)] <- newProjName
projectionsTable$newProjections <- newPrjs
updateTextInput(session, "gQC_newRnPrj", value = newProjName)
})
observeEvent(eventExpr = input$gQC_rnProj,
label = "gqc1",
handlerExpr = {
deepDebug()
rnProj = input$gQC_rnProj
projections = projections()
shiny::req(rnProj)
shiny::req(projections)
if (! rnProj %in% colnames(projections)) return(NULL)
# deepDebug()
updateTextAreaInput(session, inputId = "gQC_renameLev", value = paste(as.character(levels(factor(projections[,rnProj]))),
collapse = ", "))
})
# rename projections
.schnappsEnv$gQC_combPrj1 <- "tsne1"
.schnappsEnv$gQC_combPrj2 <- "tsne1"
.schnappsEnv$gQC_rnProj <- "tsne1"
observe(label = "ob27b", {
deepDebug()
projections <- projections()
projFactors <- projFactors()
# only factorials?
updateSelectInput(session, "gQC_raProj",
choices = projFactors,
selected = .schnappsEnv$gQC_raProj
)
updateSelectInput(session, "gQC_combPrj1",
choices = colnames(projections),
selected = .schnappsEnv$gQC_combPrj1
)
updateSelectInput(session, "gQC_combPrj2",
choices = colnames(projections),
selected = .schnappsEnv$gQC_combPrj2
)
updateSelectInput(session, "gQC_rnProj",
choices = colnames(projections),
selected = .schnappsEnv$gQC_rnProj
)
updateSelectInput(session, "oldPrj",
choices = c(colnames(projections)),
selected = .schnappsEnv$oldPrj
)
updateSelectInput(session, "delPrj",
choices = c(colnames(projectionsTable$newProjections)),
selected = .schnappsEnv$delPrj
)
updateSelectInput(session, "gQC_windProj",
choices = projFactors,
selected = .schnappsEnv$gQC_windProj
)
})
observe(label = "ob27c", {
if (DEBUG) cat(file = stderr(), "observe: gQC_combPrj1\n")
.schnappsEnv$gQC_combPrj1 <- input$gQC_combPrj1
})
observe(label = "ob27d", {
if (DEBUG) cat(file = stderr(), "observe: gQC_combPrj2\n")
.schnappsEnv$gQC_combPrj2 <- input$gQC_combPrj2
})
observe(label = "ob27e", {
if (DEBUG) cat(file = stderr(), "observe: gQC_rnProj\n")
.schnappsEnv$gQC_rnProj <- input$gQC_rnProj
})
observe(label = "ob27f", {
if (DEBUG) cat(file = stderr(), "observe: oldPrj\n")
.schnappsEnv$oldPrj <- input$oldPrj
})
observe(label = "ob27g", {
if (DEBUG) cat(file = stderr(), "observe: delPrj\n")
.schnappsEnv$delPrj <- input$delPrj
})
observe(label = "ob27h", {
if (DEBUG) cat(file = stderr(), "observe: gQC_windProj\n")
.schnappsEnv$gQC_windProj <- input$gQC_windProj
.schnappsEnv$defaultValues[["gQC_windProj"]] <- input$gQC_windProj
})
observe(label = "ob27i", {
if (DEBUG) cat(file = stderr(), "observe: gQC_raProj\n")
.schnappsEnv$gQC_raProj <- input$gQC_raProj
})
# rename levels
output$gQC_renameLev <- renderText({"text"})
# WIND ----
output$gQC_windHC <- renderPlot({
require(Wind)
# remotes::install_github("renozao/xbioc")
# library(xbioc)
if ("xbioc" %in% rownames(installed.packages())){
require(xbioc)
} else {
is_logscale <- function(x) {return(T)
cat(file = stderr(), "Please install xbioc: remotes::install_github('renozao/xbioc')")
}
}
if (DEBUG) cat(file = stderr(), "gQC_windHC started.\n")
start.time <- base::Sys.time()
on.exit({
printTimeEnd(start.time, "gQC_windHC")
if (!is.null(getDefaultReactiveDomain())) {
removeNotification(id = "gQC_windHC")
}
})
if (!is.null(getDefaultReactiveDomain())) {
showNotification("gQC_windHC", id = "gQC_windHC", duration = NULL)
}
scEx <- scEx()
projections <- projections()
pca = pcaReact()
gQC_windProj <- input$gQC_windProj
# browser()
if (is.null(projections) | is.null(scEx) | !gQC_windProj %in% colnames(projections)) {
return(NULL)
}
if (length(levels(projections[,gQC_windProj]))<3) {
if (!is.null(getDefaultReactiveDomain())) {
showNotification("Projections have less than 3 levels", id = "gQC_windHCPR", duration = 20, type = "warning")
}
}
if (.schnappsEnv$DEBUGSAVE) {
save(file = normalizePath("~/SCHNAPPsDebug/gQC_windHC.RData"), list = c(ls()))
}
# cp = load(file = "~/SCHNAPPsDebug/gQC_windHC.RData")
Y <- as.matrix(assays(scEx)[[1]])
# if(is_logscale(Y)) {
# Y = exp(Y)
# }
trueclass <- projections[,gQC_windProj]
ctStruct = tryCatch({
createRef(Y, classes = trueclass)
},error = function(e) {
if (!is.null(getDefaultReactiveDomain())) {
showNotification("Problem with WIND", type = "warning", duration = NULL)
}
cat(file = stderr(), paste("\n+++++ Error in WIND\n\t", e, "\n"))
return(NULL)
}
)
if(is.null(ctStruct)) return(NULL)
plot(ctStruct$hc, xlab="", axes=FALSE, ylab="", ann=FALSE)
})
# DoubletFinder related ----
##
if("DoubletFinder" %in% installed.packages()){
observe({
if (DEBUG) cat(file = stderr(), "GS_DF_pk update\n")
scEx = scEx()
updateNumericInput(session = session, inputId = "GS_DF_pk", value=20/ncol(scEx))
})
observeEvent(input$GS_DF_button,{
if (DEBUG) cat(file = stderr(), "GS_DF_button started.\n")
start.time <- base::Sys.time()
on.exit({
printTimeEnd(start.time, "GS_DF_button")
if (!is.null(getDefaultReactiveDomain())) {
removeNotification(id = "GS_DF_button")
}
})
if (!is.null(getDefaultReactiveDomain())) {
showNotification("GS_DF_button", id = "GS_DF_button", duration = NULL)
removeNotification(id = "GS_DF_buttonERROR")
}
scEx = scEx()
dims = input$GS_DF_dims
nRecover = input$GS_DF_nRecover
pK = input$GS_DF_pk
pN = input$GS_DF_pN
projections <- as.data.frame(projections)
newPrjs <- sessionProjections$prjs
acn = allCellNames()
if (is.null(scEx)) {
return(NULL)
}
if (is.null(projections)) {
return(NULL)
}
if (.schnappsEnv$DEBUGSAVE) {
save(file = normalizePath("~/SCHNAPPsDebug/GS_DF_button.RData"), list = c(ls()))
}
# cp = load(file = "~/SCHNAPPsDebug/GS_DF_button.RData")
dubs = tryCatch({
find_doublets_m(scEx, dims=1:dims, n_recovered=nRecover, pK=pK, pN=pN)
},
error = function(e) {
cat(file = stderr(), paste("\n\n!!!Error during detach process:", e, "\n\nDo you need to increase the memory?\n\n"))
if (!is.null(getDefaultReactiveDomain())) {
showNotification("DE_scaterPNG ERROR", id = "DE_scaterPNG_Error", duration = NULL, type = "error")
}
return(NULL)
}
)
if (ncol(newPrjs) == 0) {
newPrjs = data.frame(row.names = acn)
}
newPrjs[,colnames(dubs)] = 0
newPrjs[rownames(dubs),colnames(dubs)] = dubs
sessionProjections$prjs <- newPrjs
.schnappsEnv[["GS_DF_plot-dimension_x"]] <- "barcode"
.schnappsEnv$defaultValues[["GS_DF_plot-dimension_x"]] <- "barcode"
.schnappsEnv[["GS_DF_plot-dimension_y"]] <- colnames(dubs)[1]
.schnappsEnv$defaultValues[["GS_DF_plot-dimension_y"]] <- colnames(dubs)[1]
.schnappsEnv[["GS_DF_plot-dimension_col"]] <- colnames(dubs)[2]
.schnappsEnv$defaultValues[["GS_DF_plot-dimension_col"]] <- colnames(dubs)[2]
updateSelectizeInput(session = session, inputId = "GS_DF_plot-dimension_x", selected = "barcode")
updateSelectizeInput(session = session, inputId = "GS_DF_plot-dimension_y", selected = colnames(dubs)[1])
updateSelectizeInput(session = session, inputId = "GS_DF_plot-dimension_col", selected = colnames(dubs)[2])
})
# gc_DF_2D <-
callModule(
clusterServer,
"GS_DF_plot",
projections # ,
# reactive(input$coE_gene_id_sch)
)
}
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