The 'data' folder contains examples of how files and folders should be organized, with matching data between CDOM and FDOM files. The 'MissnamedData' folder is an example where data files are properly stuctured while having a missmatch between a CDOM and a FDOM file. To identify which file is wrongly associated, look at 'verifyMatches.csv'.
if (!require("devtools")) install.packages("devtools")
library("devtools")
devtools::install_github("RichardLaBrie/paRafac_correction")
library("paRafac.correction")
Set a working directory and run the following function to create files and download the example data
FolderCreation(WDpath=".", folder = "data", subfiles = c("CDOM","FDOM","nano"),example=T)
Try the package main function
cube=PARAFAC.cube.design(dot.number = 2, zlim = c(0,20))
The 'excitation and emission correction' .csv files (Excorr and Emcorr, respectively) have to be in the same folder as the .Rproj file. The files available with the packages are given only as example. You should use your own correction files given with your fluorometer.
FDOM, CDOM and nano files must be in their respective repositories and must respect the lower/upper case format (uppercase for CDOM and FDOM; lowercase for nano). All FDOM and CDOM files must have the same name.
->data
-> ->CDOM -> ->FDOM -> ->nano
You can see all your corrected EEMs using PlotAllEEM.R on your R plots or by exporting a pdf file containing all graphs.
Make sure that all cdom and fdom files have been correctly assigned to each other (look in verifyMatches.csv).
Export your data cube, ready to be imported into Matlab with ExportEEM.R
nEx = size(Ex,1); %Gives the number of excitation you use
nEm = size(Em,1); %Gives the number of emission you use
nSample = size(DataCube,2)/nEm; %Gives the number of sample you have
Data = DataCube;
X = reshape(DataCube, nEx, nEm, nSample); %Reshape the matrix to create a cube
X = permute(X, [3 2 1]); %Rotate the cube in the correct order for EEMs
From here, you can follow commands from DrEEM toolbox (Murphy K.R., Stedmon C.A., Graeber D. and R. Bro, Fluorescence spectroscopy and multi-way techniques. PARAFAC, Anal. Methods, 2013, DOI:10.1039/c3ay41160e.)
Add the following code to your website.
For more information on customizing the embed code, read Embedding Snippets.