R/plateHeat.R

Defines functions .plateHeat

Documented in .plateHeat

#-------------------------------------------------------------------------------
# plateHeat: create a plot showing the assay plate data
#-------------------------------------------------------------------------------

#' @title Plot plate heatmap
#' 
#' @description Plot plate heatmap, to be used with tcplPlotPlate
#' 
#' @param vals Numeric, the well values
#' @param rowi Integer, the row index
#' @param coli Integer, the column index
#' @param wllt Character, the well type
#' @param wllq Logical, the well quality
#' @param rown Integer, the number of rows on the plate
#' @param coln Integer, the number of columns on the plate
#' @param arng Numeric of length 2, the minimum and maximum values to constrain the color scale
#' @param main Character of length 1, the title/main
#'
#' @note
#' Optimized for an output with height = 20/3, width = 10, and 
#' pointsize = 10
#' 
#' @importFrom RColorBrewer brewer.pal
#' @importFrom graphics par layout plot.new plot.window text box abline axis 
#' @importFrom graphics lines rect
#' @importFrom grDevices colorRampPalette
#' @importFrom stats quantile

.plateHeat <- function(vals, rowi, coli, wllt, wllq, rown, coln, main, arng) {
  
  opar <- par()[c("pty", "mar", "mai", "family")]
  on.exit(par(opar))
  par(pty = "m", family = "mono")
  
  myPal <- colorRampPalette(rev(brewer.pal(11, "Spectral")), space = "Lab")
  
  outside <- vals < arng[1] | vals > arng[2]
  outside[is.na(outside)] <- TRUE
  vals[vals < arng[1]] <- arng[1]
  vals[vals > arng[2]] <- arng[2]
  badwlls <- cbind(rowi[!wllq], coli[!wllq])
  
  layout(matrix(c(1, 1, 2, 3), ncol = 2, byrow = TRUE),
         widths = c(9, 1),
         heights = c(1, 9))
  
  par(mar = c(0, 0, 0, 0), mai = c(0, 0, 0, 0), family = "mono")
  plot.new()
  plot.window(xlim = 0:1,
              ylim = 0:1)
  text(0.5, 0.5, main, font = 2, cex = 1.5)
  
  plot.new()
  par(mar = c(2, 3, 2, 2) + 0.1, family = "mono")
  plot.window(xlim = c(0, coln) + 0.5, 
              ylim = rev(c(0, rown) + 0.5),
              xaxs = "i", 
              yaxs = "i")
  box(which = "plot")
  abline(v = 1:(coln - 1) + 0.5)
  abline(h = 1:(rown - 1) + 0.5)
  axis(side = 3, 
       at = 1:coln, 
       tick = FALSE, 
       labels = sprintf("%02d", 1:coln), 
       cex = 0.75)
  axis(side = 2, 
       at = 1:rown, 
       tick = FALSE, 
       labels = sprintf("%02d", 1:rown), 
       cex = 0.75,
       las = 2)
  allwells <- expand.grid(1:coln, 1:rown)
  .drawCircles(x = allwells[ , 1], 
               y = allwells[ , 2], 
               r = 0.4, 
               border = "gray30")
  wcol <- myPal(500)[as.numeric(cut(c(vals, arng), breaks = 500))]
  wcol <- wcol[1:(length(vals))]
  .drawCircles(x = coli, 
               y = rowi, 
               r = 0.4, 
               border = "gray30",
               col = wcol,
               lwd = 1 + 3*outside)
  invisible(apply(badwlls, 
                  1, 
                  function(x) {
                    lines(x = c(x[2] - 0.5, x[2] + 0.5),
                          y = c(x[1] - 0.5, x[1] + 0.5))
                    lines(x = c(x[2] - 0.5, x[2] + 0.5),
                          y = c(x[1] + 0.5, x[1] - 0.5))
                  }))  
  .drawCircles(x = coli, 
               y = rowi, 
               r = 0.2, 
               border = "gray30",
               col = "white")
  text(x = coli,
       y = rowi,
       label = wllt,
       font = 2,
       col = "black",
       cex = 0.65)
  
  lgnd <- try(plot.new(), silent = TRUE)
  if (is(lgnd, "try-error")) {
    par(mar = rep(0, 4), family = "mono", new = FALSE)
    plot.new()
    warning("Could not add the legend. Please adjust device size. See",
            " ?tcplPlotPlate for more information.")
  } else {
    par(mar = c(2, 0, 2, 6) + 0.1, family = "mono")
    plot.window(xlim = c(0, 1), 
                ylim = arng, 
                xaxs = "i", 
                yaxs = "i")
    rect(0, 
         seq(arng[1], arng[2], length.out = 101)[-101],
         1,
         seq(arng[1], arng[2], length.out = 101)[-1],
         col = myPal(100),
         border = myPal(100))
    apts <- quantile(arng, probs = seq(0, 1, length.out = 10))
    axis(side = 4,
         at = apts, 
         las = 2, 
         cex = 0.5,
         labels = sprintf("%5.1f", apts))
    axis(side = 4, 
         at = range(vals, na.rm = TRUE), 
         labels = c("", ""),
         lwd = 0, 
         lwd.ticks = 2, 
         tck = -0.5)
    axis(side = 4, 
         at = range(vals, na.rm = TRUE), 
         labels = c("", ""),
         lwd = 0, 
         lwd.ticks = 2, 
         tck = 0.5)
  }
  
}

#-------------------------------------------------------------------------------
USEPA/CompTox-ToxCast-tcpl documentation built on May 5, 2019, 4:48 p.m.