R/tcplPlotPlate.R

Defines functions tcplPlotPlate

Documented in tcplPlotPlate

#-------------------------------------------------------------------------------
# tcplPlotPlate: look at the plate map for a given apid
#-------------------------------------------------------------------------------

#' @title Plot plate heatmap
#' 
#' @description
#' \code{tcplPlotPlate} generates a heatmap of assay plate data
#' 
#' @param dat data.table containing tcpl data
#' @param apid Character of length 1, the apid to plot
#' @param id Integer of length 1, the assay component id (acid) or assay
#' endpoint id (aeid), depending on level. Only need to specify for multiplexed
#' assays when more than one acid/aeid share an apid.
#' @param quant Numeric vector, the range of data to include in the legend
#' 
#' @details
#' The legend represents the range of the data supplied to dat, for the 
#' applicable ID. The additional horizontal lines on the legend indicate the
#' range of the plotted plate, to show the relation of the plate to the assay
#' as a whole. A plot with a legend specific for the given apid can be created
#' by only supplying the data for the apid of interest to 'dat'.
#' 
#' The quant parameter, by default including 99.8% of the data, 
#' allows for extreme outliers without losing resolution. Outliers in either
#' direction will be highlighted with a dark ring, as seen in the example. 
#' A NULL value for 'quant' will not restrict the data at all, and will use 
#' the full range for the legend. 
#' 
#' Wells with a well quality of 0 (only applicable for level 1 plots), will 
#' have an "X" through their center. 
#' 
#' @note
#' For the optimal output size, use width = 10, height = 10*(2/3), 
#' pointsize = 10, units = "in"
#' 
#' @examples 
#' ## Store the current config settings, so they can be reloaded at the end 
#' ## of the examples
#' conf_store <- tcplConfList()
#' tcplConfDefault()
#'  
#' d1 <- tcplLoadData(lvl = 1, fld = "acid", val = 1)
#' \dontrun{
#' tcplPlotPlate(dat = d1, apid = "09Apr2014.Plate.17")
#' }
#' 
#' ## Reset configuration
#' options(conf_store)
#'  
#' @import data.table
#' @importFrom stats quantile
#' @export


tcplPlotPlate <- function(dat, apid, id = NULL, quant = c(0.001, 0.999)) {
  
  ## Variable-binding to pass R CMD Check
  wllq <- aid <- wllt <- cndx <- nwll <- rown <- rowi <- coln <- NULL
  coli <- anm <- NULL
  
  if (length(apid) != 1) stop("'apid' must be of length 1.")
  ap <- apid
  
  lvl <- NULL
  dnames <- names(dat)
  if ("m1id" %in% dnames) {lvl <- 1L; idnm <- "acid"; vlnm <- "rval"}
  if ("m2id" %in% dnames) {lvl <- 2L; idnm <- "acid"; vlnm <- "cval"}
  if ("m3id" %in% dnames) {lvl <- 3L; idnm <- "aeid"; vlnm <- "resp"}
  if ("s1id" %in% dnames) {lvl <- 1L; idnm <- "acid"; vlnm <- "resp"}
  if (is.null(lvl)) stop("Invalid dat input.")
  
  sub <- dat[apid == ap]
  setnames(sub, c(idnm, vlnm), c("aid", "val"))
  if (!"wllq" %in% dnames) sub[ , wllq := 1L]
  
  if (is.null(id)) id <- sub[ , unique(aid)]
  if (length(id) != 1) {
    stop("Multiple ids for the given plate, must specifiy 'id'.")
  }
    
  nwlt <- sub[ , paste0(wllt, cndx)]
  
  ap_size <- do.call(if (idnm == "acid") "tcplLoadAcid" else "tcplLoadAeid", 
                     list(fld = idnm, val = id, add.fld = "assay_footprint"))
  setnames(ap_size, c("aid", "anm", "nwll"))
  
  ap_size[ , nwll := as.numeric(gsub("[^0-9]", "", nwll))]
  plate_dim <- data.table(nwll = c(6, 12, 24, 48, 96, 384, 1536),
                          coln = c(3,  4,  6,  8, 12,  24,   48),
                          rown = c(2,  3,  4,  6,  8,  16,   32))
  setkey(plate_dim, nwll)
  setkey(ap_size, nwll)
  ap_size <- plate_dim[ap_size]
  ap_size[ , rown := max(rown, sub[ , max(rowi)])]
  ap_size[ , coln := max(coln, sub[ , max(coli)])]
  
  cat_name <- ap_size[ , paste0(toupper(idnm), aid, " (", anm, ")")]
  cat_name <- paste(cat_name, ap, sep = ": ")
  
  if (is.null(quant)) {
    arng <- dat[get(idnm) == id, range(get(vlnm), na.rm = TRUE)]
  } else {
    arng <- dat[get(idnm) == id, 
                quantile(get(vlnm), c(0.001, 0.999), na.rm = TRUE)]
  }
  
  with(data = sub[aid == id],
       .plateHeat(vals = val,
                  rowi = rowi, 
                  coli = coli, 
                  wllt = nwlt,
                  wllq = wllq,
                  rown = ap_size[ , rown], 
                  coln = ap_size[, coln], 
                  main = cat_name, 
                  arng = arng))
  
}

#-------------------------------------------------------------------------------
USEPA/CompTox-ToxCast-tcpl documentation built on May 5, 2019, 4:48 p.m.