R/rnaf.R

#' @name rnaf
#' @title 
#'   Model of positional bias that can arise when RNA-seq is performed
#'   using protocols relying on RNA fragmentation.
#' @description 
#'   This positional bias model was estimated in Li and Jiang (2012).
#'   With RNA fragmentation, reads are more likely to have come from the middle
#'   of the transcript than either end. The probabilities included in this 
#'   dataset were estimated from Supplementary Figure S3 in Li and Jiang's 
#'   manuscript. Data points from the figure were inferred and exported as CSV 
#'   files using WebPlotDigitizer. The CSV files and the code used to process 
#'   them and create the datasets are available in the Polyester GitHub
#'   repository (\url{https://github.com/alyssafrazee/polyester}).
#' @docType data
#' @format 
#'   data frame with 100 rows and 2 columns. Column 1 is position along a
#'   transcript (in percent), while Column 2 is the probability of getting a 
#'   fragment at that position. Column 2 sums to 1.
#' @references 
#'   Li W and Jiang T (2012): Transcriptome assembly and isoform expression
#'   level estimation from biased RNA-Seq reads. Bioinformatics 28(22):
#'   2914-2921.
#' 
#'   Rohatgi A (2014): WebPlotDigitizer: Version 3.4 of WebPlotDigitizer. 
#'   ZENODO. 10.5281/zenodo.11835
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alyssafrazee/polyester documentation built on Sept. 17, 2021, 8:54 a.m.