In lwjohnst86/seminar2016: Slides and Data Analysis for PhD Seminar Fall 2016
Words: 270 / 250
Fatty acid (FA) composition in four serum lipid fractions and the pathogenesis
of diabetes: The Prospective Metabolism and Islet Cell Evaluation (PROMISE)
cohort
Luke W. Johnston
Background: Evidence suggests specific FAs within serum lipid fractions
contribute uniquely to the risk for diabetes. However, limited longitudinal data
exist on the role of the FA composition in different lipid fractions and the
underlying pathogenesis of diabetes. Our aim was to study the longitudinal
associations of FA composition in four lipid fractions with 6-yr changes in
insulin sensitivity (IS) and beta-cell function.
Methods: Adults at-risk for diabetes in PROMISE had glucose and insulin
measured from an oral glucose tolerance test at 3 visits over 6-yrs (n=477).
Baseline fasting FA in phospholipid (PL), cholesteryl ester, triacylglycerol
(TAGFA), and non-esterified (NEFA) fractions were quantified using
thin-layer-chromatography and gas-chromatography. The inverse of HOMA-IR
(1/HOMA-IR) and the Matsuda index assessed IS; the Insulinogenic Index over
HOMA-IR (IGI/IR) and the Insulin Secretion-Sensitivity Index-2 (ISSI-2) assessed
beta-cell function. Partial least squares and generalized estimating equations
was conducted adjusting for visit, sex, ethnicity, baseline age, waist, NEFA,
and ALT.
Results: IS and beta-cell function declined by 14-27% over 6-yrs. Total
NEFA associated negatively with beta-cell function. In PL, cis-vaccenate
associated positively with IS while palmitate associated with declines in
beta-cell function. Four TAGFA (14:0, 16:0, 14:1n7, 16:1n17) clustered together
to associate with lower IS. Monounsaturated TAGFA tended to associate positively
with IS.
Conclusions: These findings reveal fraction and FA specific associations
with core abnormalities underlying diabetes, providing a comprehensive view of
FA in diabetes pathogenesis. In particular, within the TAGFA, the FA cluster may
be indicative of higher de novo lipogenesis of carbohydrates. We hope the
results encourage further research on the FA with particularly strong
associations, as many of these FA are not well studied.
lwjohnst86/seminar2016 documentation built on May 21, 2019, 9:15 a.m.
R Package Documentation
Browse R Packages
We want your feedback!
Note that we can't provide technical support on individual packages. You should contact the package authors for that.
Words: 270 / 250
Fatty acid (FA) composition in four serum lipid fractions and the pathogenesis of diabetes: The Prospective Metabolism and Islet Cell Evaluation (PROMISE) cohort
Luke W. Johnston
Background: Evidence suggests specific FAs within serum lipid fractions contribute uniquely to the risk for diabetes. However, limited longitudinal data exist on the role of the FA composition in different lipid fractions and the underlying pathogenesis of diabetes. Our aim was to study the longitudinal associations of FA composition in four lipid fractions with 6-yr changes in insulin sensitivity (IS) and beta-cell function.
Methods: Adults at-risk for diabetes in PROMISE had glucose and insulin measured from an oral glucose tolerance test at 3 visits over 6-yrs (n=477). Baseline fasting FA in phospholipid (PL), cholesteryl ester, triacylglycerol (TAGFA), and non-esterified (NEFA) fractions were quantified using thin-layer-chromatography and gas-chromatography. The inverse of HOMA-IR (1/HOMA-IR) and the Matsuda index assessed IS; the Insulinogenic Index over HOMA-IR (IGI/IR) and the Insulin Secretion-Sensitivity Index-2 (ISSI-2) assessed beta-cell function. Partial least squares and generalized estimating equations was conducted adjusting for visit, sex, ethnicity, baseline age, waist, NEFA, and ALT.
Results: IS and beta-cell function declined by 14-27% over 6-yrs. Total NEFA associated negatively with beta-cell function. In PL, cis-vaccenate associated positively with IS while palmitate associated with declines in beta-cell function. Four TAGFA (14:0, 16:0, 14:1n7, 16:1n17) clustered together to associate with lower IS. Monounsaturated TAGFA tended to associate positively with IS.
Conclusions: These findings reveal fraction and FA specific associations with core abnormalities underlying diabetes, providing a comprehensive view of FA in diabetes pathogenesis. In particular, within the TAGFA, the FA cluster may be indicative of higher de novo lipogenesis of carbohydrates. We hope the results encourage further research on the FA with particularly strong associations, as many of these FA are not well studied.
R Package Documentation
Browse R Packages
We want your feedback!
Note that we can't provide technical support on individual packages. You should contact the package authors for that.
Embedding an R snippet on your website
Add the following code to your website.
For more information on customizing the embed code, read Embedding Snippets.