In mikeod38/ProvidenciaChemo:

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Fig. 4. Modulation of octanol avoidance by Providencia requires the OCTR-1 OA receptor in the ASH/ASI sensory neurons.\ a-b) Reversal response latency of animals of the indicated genotypes grown on the shown bacteria in control conditions of NGM + 0.5% L-Tyr (a) or supplemented with TA + 0.5% L-Tyr (b) to 100% octanol using SOS assays. Each dot is the response time of a single worm. Y-axis is log~10~-scaled for these log-normal distributed data, and normalized to the indicated control group for each experimental day. Numbers in parentheses indicate the number of worms tested in assays over at least 3 independent days. Boxplot indicates median and quartiles, whiskers indicate the data range, excluding outliers. Gray thin and thick vertical bars at right indicate Bayesian 95% and 66% credible intervals for the difference of means, respectively. P-values between indicated conditions are from a LMM with Tukey-type multivariate-t adjustment.\ c) (Left) Cartoon depicting assay setup of the short-range bacterial choice assay. (Right) Preference index of animals grown on the indicated bacteria for the test bacteria JUb39. Each dot represents one assay of at least 10 animals; assays were performed over at least 4 independent days. Y-axis is on log-odds (logit) scale. Errors are SEM. Gray thin and thick vertical bars at right indicate Bayesian 95% and 66% credible intervals, respectively. P-values represent difference of means relative to JUb39-grown animals from a GLMM with Dunnett-type multivariate-t adjustment.\ d) Cartoon of working model. JUb39 colonizes the C. elegans intestine and produces TA via the TyrDC and AdcA enzymes. TA is converted to OA by C. elegans TBH-1 and acts via the ASH neuron-expressed OCTR-1 OA receptor to modulate octanol avoidance and food choice.

mikeod38/ProvidenciaChemo documentation built on April 6, 2020, 11:57 p.m.