README.md

In nghiavtr/mascotParser: Parse Mascot results file (*.dat) from both PMF and MSMS searches

mascotParser

A parser for Mascot search results ( both PMF and MSMS) in R package.

How to install "mascotParser"

From Github using R:

install.packages("devtools")
library("devtools")
install_github("mascotParser","nghiavtr")
library("mascotParser")

1. Example for parsing MSMS Mascot search result files:

library(MascotParser)
filename <- "MSMS_Mascot_search_result.dat";

parse the dat file

myParse <- parse(new("MascotParser"),filename)

Get MS1 peaks

myParse@qexp
myParse@qintensity

how many query of MS2 in this data set?

myParse@MSMSnumQuery

how many hits and hit scores from these query?

myParse@PIHnumHits
myParse@PIHhitScores

Extract information of MS2 peaks from query 3

myms2=getMSMSqueryInfo(myParse,3)
myms2$mz
myms2$intensity

plot MS1 mass spetrum

plotSpec(myParse,specTitle=NULL,queryId=NULL)

plot mass spectrum of query 1

plotSpec(myParse,specTitle=NULL,queryId=1)

draw a panel plot of MS2 peaks from query 1 and query 2

mz1=getMSMSqueryInfo(myParse,1)$mz
int1=getMSMSqueryInfo(myParse,1)$intensity
mz2=getMSMSqueryInfo(myParse,2)$mz
int2=getMSMSqueryInfo(myParse,2)$intensity
plotSpecPanel(mz1,int1,mz2,int2,xlimVal=500,plotTitle="test title")

2. Example for parsing PMF Mascot search result files:

library(MascotParser)
filename <- "PMF_Mascot_search_result.dat";

parse the dat file

myParse <- parse(new("MascotParser"),filename)

get unassigned peaks from the search

negPeaks <- myParse@qexp[getUnassignedPeaks(myParse, scoreThres=MascotThres)]

plot PMF mass spetrum

plotSpec(myParse,specTitle=NULL,queryId=NULL)

nghiavtr/mascotParser documentation built on May 23, 2019, 4:42 p.m.