normalizeFrames: Normalize the values of a 'Frames' object

View source: R/auxiliary.R

normalizeFramesR Documentation

Normalize the values of a Frames object

Description

Applies a transformation to the Frames object in a way that the intensities throughout the acquisition are normalized overall in term of pixel values sums. It can be used to compensate for example a global change in the illumination values, e.g. due to changed acquisition conditions in experiments that span long timescales.

Usage

normalizeFrames(frames, normFun = "median")

Arguments

frames

A Frames object to normalize

normFun

The normalization function chosen. Can be one of mean or median

Value

A Frames object with normalized pixel values.

Author(s)

Federico Marini, marinif@uni-mainz.de, 2014

Examples

data(MesenteriumSubset)
normalizeFrames(MesenteriumSubset,normFun="median") 

federicomarini/flowcatchR documentation built on May 3, 2024, 11:15 p.m.