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HDStIM: High Dimensional Stimulation Immune Mapping ('HDStIM')
A method for identifying responses to experimental stimulation in mass or flow cytometry that uses high dimensional analysis of measured parameters and can be performed with an end-to-end unsupervised approach. In the context of in vitro stimulation assays where high-parameter cytometry was used to monitor intracellular response markers, using cell populations annotated either through automated clustering or manual gating for a combined set of stimulated and unstimulated samples, 'HDStIM' labels cells as responding or non-responding. The package also provides auxiliary functions to rank intracellular markers based on their contribution to identifying responses and generating diagnostic plots.
Package details |
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| Author | Rohit Farmer [aut, cre] (<https://orcid.org/0000-0003-4197-3047>), Richard Apps [aut] (<https://orcid.org/0000-0001-5140-0141>), John Tsang [aut, pdr] (<https://orcid.org/0000-0003-3186-3047>) |
| Maintainer | Rohit Farmer <rohit.farmer@gmail.com> |
| License | CC0 | file LICENSE |
| Version | 0.1.0 |
| URL | https://github.com/niaid/HDStIM https://niaid.github.io/HDStIM/ |
| Package repository | View on CRAN |
| Installation |
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