karlen: Real-Time PCR Data Sets by Karlen et al. (2007)

Real-time quantitative polymerase chain reaction (qPCR) data sets by Karlen et al. (2007) <doi:10.1186/1471-2105-8-131>. Provides one single tabular tidy data set in long format, encompassing 32 dilution series, for seven PCR targets and four biological samples. The targeted amplicons are within the murine genes: Cav1, Ccn2, Eln, Fn1, Rpl27, Hspg2, and Serpine1, respectively. Dilution series: scheme 1 (Cav1, Eln, Hspg2, Serpine1): 1-fold, 10-fold, 50-fold, and 100-fold; scheme 2 (Ccn2, Rpl27, Fn1): 1-fold, 10-fold, 50-fold, 100-fold and 1000-fold. For each concentration there are five replicates, except for the 1000-fold concentration, where only two replicates were performed. Each amplification curve is 40 cycles long. Original raw data file is Additional file 2 from "Statistical significance of quantitative PCR" by Y. Karlen, A. McNair, S. Perseguers, C. Mazza, and N. Mermod (2007) <https://static-content.springer.com/esm/art%3A10.1186%2F1471-2105-8-131/MediaObjects/12859_2006_1503_MOESM2_ESM.ZIP>.

install.packages("karlen")

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