shearCorrection: Correct for DNA shearing

In ddPCRclust: Clustering algorithm for ddPCR data

Description

Longer DNA templates produce a lower droplet count due to DNA shearing. This function normalizes the ddPCRclust result based on a stable marker of different lengths to negate the effect of differences in the lengths of the actual markers of interest. (Work in progress)

Usage

shearCorrection(counts, lengthControl, stableControl)

Arguments

counts	The counts per marker as provided by calculateCPDs.
lengthControl	The name of the length Control. If the template name is for example CPT2, the name in the template should be CPT2-125, where 125 represents the number of basepairs.
stableControl	The name of the stable Control used as a reference for this experiment.

Value

A linear regression model fitting the length vs ln(ratio) (see lm for details on linear regression).

ddPCRclust documentation built on Nov. 8, 2020, 6:06 p.m.