inst/doc/pRolocGUI.R

In pRolocGUI: Interactive visualisation of spatial proteomics data

## ----env, echo=FALSE----------------------------------------------------------
library("BiocStyle")

## ----loadPkgs, message = FALSE, warning = FALSE-------------------------------
library("pRolocGUI")
library("pRolocdata")

## ----loadData, echo = TRUE, message = FALSE, warning = FALSE------------------
data(hyperLOPIT2015) 

## ----example, eval = FALSE, echo = TRUE---------------------------------------
#  pRolocVis(object = hyperLOPIT2015, fcol = "markers")

## ----pca1, eval = FALSE, echo = TRUE------------------------------------------
#  pRolocVis(object = hyperLOPIT2015, fcol = "markers")

## ----compare, eval = FALSE, echo = TRUE---------------------------------------
#  data(hyperLOPIT2015ms3r1)
#  data(hyperLOPIT2015ms3r2)
#  mydata <- MSnSetList(list(hyperLOPIT2015ms3r1, hyperLOPIT2015ms3r2))
#  pRolocVis(mydata, app = "compare", fcol = "markers")

## ----compare2, eval=FALSE, echo=TRUE------------------------------------------
#  data("hyperLOPITU2OS2018")
#  data("lopitdcU2OS2018")
#  xx <- MSnSetList(list(hyperLOPITU2OS2018, lopitdcU2OS2018))
#  if (interactive()) {
#    pRolocVis(xx, app = "compare", fcol = c("markers", "final.assignment"))
#  }

## ----aggvar, eval = FALSE, echo = TRUE, message = FALSE, warning = FALSE------
#  ## load PSM data
#  data("hyperLOPIT2015ms2psm")
#  
#  ## Visualise the PSMs per to protein group
#  pRolocVis(hyperLOPIT2015ms2psm, app = "aggregate", fcol = "markers",
#            groupBy = "Protein.Group.Accessions")

## ----aggvar2, eval = FALSE, echo = TRUE, message = FALSE, warning = FALSE-----
#  ## Combine PSM data to peptides
#  hl <- combineFeatures(hyperLOPIT2015ms2psm,
#                        groupBy = fData(hyperLOPIT2015ms2psm)$Sequence,
#                        method = median)
#  
#  ## Visualise peptides according to protein group
#  pRolocVis(hyperLOPIT2015ms2psm, app = "aggregate", fcol = "markers",
#            groupBy = "Protein.Group.Accessions")