Description Usage Arguments Objects from the Class Slots Extends Methods Author(s) References See Also Examples
Performs a parametric bootstrap test (by adding independent Gaussian noise) to determine whether the clusters found by an unsupervised method appear to be robust in a given data set.
1  PerturbationClusterTest(data, FUN, nTimes=100, noise=1, verbose=TRUE, ...)

data 
A data matrix, numerical data frame, or

FUN 
A 
... 
Additional arguments passed to the classifying function, 
noise 
An optional numeric argument; the standard deviation of the mean zero Gaussian noise added to each measurement during each bootstrap. Defaults to 1. 
nTimes 
The number of bootstrap samples to collect. 
verbose 
A logical flag 
Objects should be created using the PerturbationClusterTest
function, which performs the requested bootstrap on the
clusters. Following the standard R paradigm, the resulting object can be
summarized and plotted to determine the results of the test.
f
:A function
that, given a data matrix,
returns a vector of cluster assignments. Examples of functions
with this behavior are cutHclust
,
cutKmeans
, cutPam
, and
cutRepeatedKmeans
.
noise
:The standard deviation of the Gaussian noise added during each bootstrap sample.
nTimes
:An integer, the number of bootstrap samples that were collected.
call
:An object of class call
, which records
how the object was produced.
result
:Object of class matrix
containing, for
each pair of columns in the original data, the number of times
they belonged to the same cluster of a bootstrap sample.
Class ClusterTest
, directly. See that class for
descriptions of the inherited methods image
and hist
.
signature(object = PerturbationClusterTest)
:
Write out a summary of the object.
Kevin R. Coombes krc@silicovore.com
Kerr MK, Churchill GJ.
Bootstrapping cluster analysis: Assessing the reliability of
conclusions from microarray experiments.
PNAS 2001; 98:89618965.
BootstrapClusterTest
,
ClusterTest
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37  showClass("PerturbationClusterTest")
## simulate data from two different groups
d1 < matrix(rnorm(100*30, rnorm(100, 0.5)), nrow=100, ncol=30, byrow=FALSE)
d2 < matrix(rnorm(100*20, rnorm(100, 0.5)), nrow=100, ncol=20, byrow=FALSE)
dd < cbind(d1, d2)
cols < rep(c('red', 'green'), times=c(30,20))
colnames(dd) < paste(cols, c(1:30, 1:20), sep='')
## peform your basic hierarchical clustering...
hc < hclust(distanceMatrix(dd, 'pearson'), method='complete')
## bootstrap the clusters arising from hclust
bc < PerturbationClusterTest(dd, cutHclust, nTimes=200, k=3, metric='pearson')
summary(bc)
## look at the distribution of agreement scores
hist(bc, breaks=101)
## let heatmap compute a new dendrogram from the agreement
image(bc, col=blueyellow(64), RowSideColors=cols, ColSideColors=cols)
## plot the agreement matrix with the original dendrogram
image(bc, dendrogram=hc, col=blueyellow(64), RowSideColors=cols, ColSideColors=cols)
## bootstrap the results of Kmeans
kmc < PerturbationClusterTest(dd, cutKmeans, nTimes=200, k=3)
image(kmc, dendrogram=hc, col=blueyellow(64), RowSideColors=cols, ColSideColors=cols)
## contrast the behavior when all the data comes from the same group
xx < matrix(rnorm(100*50, rnorm(100, 0.5)), nrow=100, ncol=50, byrow=FALSE)
hct < hclust(distanceMatrix(xx, 'pearson'), method='complete')
bct < PerturbationClusterTest(xx, cutHclust, nTimes=200, k=4, metric='pearson')
summary(bct)
image(bct, dendrogram=hct, col=blueyellow(64), RowSideColors=cols, ColSideColors=cols)
## cleanup
rm(d1, d2, dd, cols, hc, bc, kmc, xx, hct, bct)

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