filter_debris_flow: Flag debris in flow cytometry data.

In Cleanet: Automated Doublet Detection and Classification for Cytometry Data

Flag debris in flow cytometry data.

Description

Detect events in the lower left corner of FSC-A/SSC-A plots. This function aims for high specificity, but not high sensitivity: for Cleanet's purposes, it suffices to deplete debris, even if not all of it is eliminated.

Usage

filter_debris_flow(df, sum_max = 50000, cols = c("FSC-A", "SSC-A"))

Arguments

df	A data frame containing scattering channels and protein expression data.
sum_max	Numeric; events whose sum of FSC-A and SSC-A is smaller than this value are flagged.
cols	Names of columns to use. This function is intended for use with the area channel of forward and side scatter.

Value

A binary array with the same length as the number of rows in df. TRUE for debris, FALSE for everything else.

Cleanet documentation built on April 4, 2025, 12:17 a.m.