V2: Line-scan data of HEK-293 cells expressing Venus (EYFP)...

In FCSlib: A Collection of Fluorescence Fluctuation Spectroscopy Analysis Methods

Description

This data set consists on a raster line scan performed over HEK-293 cells expressing dimers of the fluorescent protein Venus, also known as SEYFP-F46L. The scan line is 64 pixels long, and the scanning direction is from the cytoplasm to the nucleus, across the nuclear envelope. A pixel size of 50 nm was used, as well as a pixel dwell time of 12.5 us and a line scan time of 1.925 ms. Fluorescence excitation was provided by a 488 nm laser at 0.1 Fluorescence intensity data was collected using the photon-counting mode in an Olympus FV1000 Upright BX61WI confocal microscope.

Usage

data(V2)

Format

A matrix with 64 rows and 25000 columns

Details

To analyze this data set with FCSlib, import by typing: readFileTiff("V2.tif")[,,1]

FCSlib documentation built on Nov. 27, 2020, 5:09 p.m.