FRAPdata: FRAP analysis dataset

In FRApp: FRAP Data Analysis Using Nonlinear Mixed Effect Models with 'shiny'

FRAP analysis dataset

Description

The dataset provides 65 measures of fluorescence intensity equally spaced in time over a period of 100 seconds for 132 dendritic spines, for a total of 8580 observations. Data presents a nested hierarchical structure: spines belongs to 54 neurons, grouped in 6 cultures. The number of spines within neurons, and of neurons within cultures is not constant. Experimental condition is applied to 28 neurons (CAP2-shRNA, 71 spines), whereas 26 are controls (SCR, 61 spines). The dataset is a subset of the original version where spines with fluorescence intensity larger than 0.6 at time 0 have been excluded. For a comprehensive description of the data and of the data cleaning steps, please refer to the paper below.

Usage

FRAPdata

Format

FRAPdata

A data frame with 8580 rows and 6 columns:

Culture.id

Culture id, from 1 to 6

genetic.id

Experimental condition, SCR for control and SH for CAP2-shRNA

neuron

Neuron id and experimental condition, nested within cultures

spine.ID

Spine id, nested within neurons

time

Time, from 0 to 99.84

y

Fluorescence intensities

Source

Di Credico, G., Pelucchi, S., Pauli, F. et al. Nonlinear mixed-effects models to analyze actin dynamics in dendritic spines. Sci Rep 15, 5790 (2025). https://doi.org/10.1038/s41598-025-87154-w

FRApp documentation built on April 4, 2025, 12:19 a.m.