ACL394: Time Dependent Dose Response of Yeast Cells to Mating...

Description Usage Format References

Description

This datasets was generated by Cell-ID, from an experiment done in 2004 by Alejandro Colman-Lerner and Andrew Gordon at the Molecular Science Institute (MSI). Saccharomices cerevisiae yeast cells of strain TCY3154 (MATa, bar1, prm1::Pprm1-YFP::HIS+, trp1::Pact1-CFP::TRP1) where stimulated with different doses of alpha-factor pheromone 10 minutes before the first time point. Images where adquired every 15 minutes for 3.5 hours. In the dataset there are 3 positions per treatment. The strain TCY3154 was derived from ACL394, a W303 derivative.

data(ACL394) loads the unfiltered dataset, while data(ACL394filtered) loads the dataset with filters applied.

pos1.cell.counter is a data.frame as returned by ImageJ's plugin Cell Counter.

pdata is a data.frame with the description of each position.

Usage

1
X

Format

a cell.data object

References

Colman-Lerner et al. (2005). "Regulated cell-to-cell variation in a cell-fate decision system." Nature 437(7059):699-706.


RcellData documentation built on May 1, 2019, 8:39 p.m.