View source: R/plotPat.TcGSA.R
plotPat.TcGSA | R Documentation |
This function plots a series of gene sets dynamic trends heatmaps. One heatmap is drawn for each patient. NOT IMPLEMENTED YET (TODO)
plotPat.TcGSA( x, threshold = 0.05, myproc = "BY", nbsimu_pval = 1e+06, expr, Subject_ID, TimePoint, baseline = NULL, only.signif = TRUE, group.var = NULL, Group_ID_paired = NULL, ref = NULL, group_of_interest = NULL, FUNcluster = NULL, clustering_metric = "euclidian", clustering_method = "ward", B = 500, max_trends = 4, aggreg.fun = "median", na.rm.aggreg = TRUE, methodOptiClust = "firstSEmax", verbose = TRUE, clust_trends = NULL, N_clusters = NULL, myclusters = NULL, label.clusters = NULL, prev_rowCL = NULL, descript = TRUE, plotAll = TRUE, color.vec = c("darkred", "#D73027", "#FC8D59", "snow", "#91BFDB", "#4575B4", "darkblue"), legend.breaks = NULL, label.column = NULL, time_unit = "", cex.label.row = 1, cex.label.column = 1, margins = c(5, 25), heatKey.size = 1, dendrogram.size = 1, heatmap.height = 1, heatmap.width = 1, cex.clusterKey = 1, cex.main = 1, horiz.clusterKey = TRUE, main = NULL, subtitle = NULL, ... )
x |
a tcgsa object. |
threshold |
the threshold at which the FDR or the FWER should be controlled. |
myproc |
a vector of character strings containing the names of the
multiple testing procedures for which adjusted p-values are to be computed.
This vector should include any of the following: " |
nbsimu_pval |
the number of observations under the null distribution to
be generated in order to compute the p-values. Default is |
expr |
either a matrix or dataframe of gene expression upon which
dynamics are to be calculated, or a list of gene sets estimation of gene
expression. In the case of a matrix or dataframe, its dimension are n
x p, with the p sample in column and the n genes in row.
In the case of a list, its length should correspond to the number of gene
sets under scrutiny and each element should be an 3 dimension array of
estimated gene expression, such as for the list returned in the
|
Subject_ID |
a factor of length p that is in the same order as the
columns of |
TimePoint |
a numeric vector or a factor of length p that is in
the same order as |
baseline |
a character string which is the value of |
only.signif |
logical flag for plotting only the significant gene sets.
If |
group.var |
in the case of several treatment groups, this is a factor of
length p that is in the same order as |
Group_ID_paired |
a character vector of length p that is in the
same order as |
ref |
the group which is used as reference in the case of several
treatment groups. Default is |
group_of_interest |
the group of interest, for which dynamics are to be
computed in the case of several treatment groups. Default is |
FUNcluster |
the clustering function used to agglomerate genes in
trends. Default is |
clustering_metric |
character string specifying the metric to be used
for calculating dissimilarities between observations in the hierarchical
clustering when |
clustering_method |
character string defining the agglomerative method
to be used in the hierarchical clustering when |
B |
integer specifying the number of Monte Carlo ("bootstrap") samples
used to compute the gap statistics. Default is |
max_trends |
integer specifying the maximum number of different clusters
to be tested. Default is |
aggreg.fun |
a character string such as |
na.rm.aggreg |
a logical flag indicating whether |
methodOptiClust |
character string indicating how the "optimal"" number
of clusters is computed from the gap statistics and their standard
deviations. Possible values are |
verbose |
logical flag enabling verbose messages to track the computing
status of the function. Default is |
clust_trends |
object of class ClusteredTrends containing
already computed trends for the plotted gene sets. Default is |
N_clusters |
an integer that is the number of clusters in which the
dynamics should be regrouped. The cutoff of the clustering tree is
automatically calculated accordingly. Default is |
myclusters |
a character vector of colors for predefined clusters of the
represented gene sets, with as many levels as the value of |
label.clusters |
if |
prev_rowCL |
a hclust object, such as the one return by the
present plotting function (see Value) for instance. If not |
descript |
logical flag indicating that the description of the gene sets
should appear after their name on the right side of the plot if |
plotAll |
logical flag indicating whether a first heatmap with the median
over all the patients should be plotted, or not. Default is |
color.vec |
a character strings vector used to define the color
palette used in the plot. Default is
|
legend.breaks |
a numeric vector indicating the splitting points for
coloring. Default is |
label.column |
a vector of character strings with the labels to be
displayed for the columns (i.e. the time points). Default is |
time_unit |
the time unit to be displayed (such as |
cex.label.row |
a numerical value giving the amount by which row labels
text should be magnified relative to the default |
cex.label.column |
a numerical value giving the amount by which column
labels text should be magnified relative to the default |
margins |
numeric vector of length 2 containing the margins (see
|
heatKey.size |
the size of the color key for the heatmap fill. Default
is |
dendrogram.size |
the horizontal size of the dendrogram. Default is
|
heatmap.height |
the height of the heatmap. Default is |
heatmap.width |
the width of the heatmap. Default is |
cex.clusterKey |
a numerical value giving the amount by which the
clusters legend text should be magnified relative to the default |
cex.main |
a numerical value giving the amount by which title text
should be magnified relative to the default |
horiz.clusterKey |
a logical flag; if |
main |
a character string for an optional title. Default is
|
subtitle |
a character string for an optional subtitle. Default is
|
... |
other parameters to be passed through to plotting functions. |
On the heatmap, each line corresponds to a gene set, and each column to a time point.
First a heatmap is computed on all the patients (see plot.TcGSA
and clustTrend
) to define the clustering. Then, the clustering
and coloring thus defined on all the patients are consistently used in the
separate heatmaps that are plotted by patient.
If expr
is a matrix or a dataframe, then the "original" data are
plotted. On the other hand, if expr
is a list returned in the
'Estimations'
element of TcGSA.LR
, then it is those
"estimations" made by the TcGSA.LR
function that are plotted.
If descript
is FALSE
, the second element of margins
can
be reduced (for instance use margins = c(5, 10)
), as there is not so
much need for space in order to display only the gene set names, without
their description.
The median shown in the heatmap uses the respectively standardized (reduced and centered) expression of the genes over the patients.
An object of class hclust which describes the tree produced by the clustering process. The object is a list with components:
merge
an n-1 by 2 matrix. Row i of
merge
describes the merging of clusters at step i of the clustering.
If an element j in the row is negative, then observation -j was
merged at this stage. If j is positive then the merge was with the
cluster formed at the (earlier) stage j of the algorithm. Thus
negative entries in merge indicate agglomerations of singletons, and positive
entries indicate agglomerations of non-singletons.
height
a set of n-1 real values (non-decreasing for
ultrametric trees). The clustering height: that is, the value of the
criterion associated with the Ward clustering method.
order
a vector giving the permutation of the original
observations suitable for plotting, in the sense that a cluster plot using
this ordering and matrix merge will not have crossings of the branches.
labels
the gene sets name.
call
the call which produced the result clustering:
hclust(d = dist(map2heat, method = "euclidean"), method = "ward.D2")
method "ward.D2", as it is the clustering method that has been used for clustering the gene set trends.
dist.method
"euclidean", as it is the distance that has been used
for clustering the gene set trends.
legend.breaks
a numeric vector giving the splitting points used
for coloring the heatmap. If plot
is FALSE
, then it is
NULL
.
myclusters
a character vector of colors for clusters of the
represented gene sets, with as many levels as the value of N_clusters
.
If no clusters were represented, than this is NULL
.
ddr
a dendrogram object with the reordering used for the
heatmap. See heatmap.2
function from package
gplots
.
clustersExport
a data frame with 2 variables containing the two
following variables :
GeneSet
: the gene sets
clustered.
Cluster
: the cluster they belong to.
The data
frame is order by the variable Cluster
.
Boris P. Hejblum
Hejblum BP, Skinner J, Thiebaut R, (2015) Time-Course Gene Set Analysis for Longitudinal Gene Expression Data. PLOS Comput. Biol. 11(6):e1004310. doi: 10.1371/journal.pcbi.1004310
plot.TcGSA
,
TcGSA.LR
, hclust
if(interactive()){ data(data_simu_TcGSA) tcgsa_sim_1grp <- TcGSA.LR(expr=expr_1grp, gmt=gmt_sim, design=design, subject_name="Patient_ID", time_name="TimePoint", time_func="linear", crossedRandom=FALSE) plotPat.TcGSA(x=tcgsa_sim_1grp, expr=expr_1grp, Subject_ID=design$Patient_ID, TimePoint=design$TimePoint, B=100, time_unit="H" ) plotPat.TcGSA(x=tcgsa_sim_1grp, expr=tcgsa_sim_1grp$Estimations, Subject_ID=design$Patient_ID, TimePoint=design$TimePoint, baseline=1, B=100, time_unit="H" ) }
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