"setD" estimates the minimal sample size given the users precision requirements including the accepted errors of the first and second type.
1 2 3 
nmax 
maximum available number of organisms that can be tested per treatment. The calculation of the optimal number will only be done if this number is high enough to generate the response of at least one organism under control conditions (nmax*SL.p > 1). Otherwise a warning message is issued. 
SL.p 
spontaneous lethality in percent. The function "spoD" can be used to calculate SL.p from own experimental data. 
immunity.p 
Immunity in percent. A population of biological objects might contain a subpopulation which shows no reaction at all (is immune). immunity.p gives the size of the subpopulation in percent of total population size. The concentrationresponse curve always has [100%  IY] as maximum. EC values are affected by immunity.p only if risktype=3 is used. 
risk.type 
Choose one of (1,2,3) to select a risk type (see reference for more detail): 1: Total risk (TR): The total risk is the total response expressed as percentage of affected biological units among all treated units. Spontaneous lethality and immunity are ignored. 2: Added risk (AR): The reference frame is restricted below and above by spontaneous lethality (SL) and immunity (IY). Only the response above the SL is considered as an effect. Using AR, the total response associated with a target effect of size xx and a spontaneous lethality SL is xx + SL. 3: Extra risk (ER): The reference frame is the interval from SL to (100%IY). Using ER, the total response associated with a target effect of size xx is SL + 0.01 * xx * (100%SLIY). 
target.EC.p 
The target response in percent (e.g. 10% to calculate EC10). Only a single value is allowed. Note that the interpretation of target.EC depends on the risktype setting. 
plot 
There are three possibilities: plot = FALSE: no plots. plot ="single": Creates one plot showing the distributions under no treatment and under treated conditions with the optimal number of cases. Additionally, the actual rates of the type I and type II error are given. plot ="all": In addition to the "single" plot this option provides a sample size estimation for all possible target values. This gives an impression which possibilities of detection exist under the chosen conditions. This option may need a lot of computer capacity and time. It should not be activated in general. 
alpha.p 
alpha & beta: size of the type I and type II in percent. The default global settings for alpha and beta are alpha = 5 percent and beta = 20 percent. Increasing the default error rates is not recommended. The accepted error rates are global parameters and affect all calculations. 
beta.p 
see alpha.p 
print.result 
If omitted, the result is written to "02.sample size.txt" in the calling directory, if a file name is given, the result is written to that file, if FALSE, nothing is written 
number.organisms 
sample size per treatment, substance and run 
spontaneous.lethality 
assumed spontaneous lethality in % 
immunity 
assumed immunity in % 
delta.to.zero 
total response (= 
risk.type 
risk type, see arguments 
target.EC 
the target response in percent 
exact.alpha 
exact alpha error when testing H0: p(response) =

exact.beta 
exact beta error, given the parameters assumed and
number of organisms = 
Nadia Keddig & Werner Wosniok
Optimal test design for binary response data: the example of the Fish Embryo Toxicity Test. Submitted.
1 2 3 4 5 6  # sample size calculation per treatment and experimental run
setD(nmax=350,SL.p=5.5,immunity.p=0,risk.type=2,target.EC=10,
alpha.p=5,beta.p=20,plot="single")
setD(nmax=350,SL.p=3,target.EC=5,plot="FALSE")
setD(nmax=350,SL.p=3,target.EC=5,plot="FALSE",print.result="setD.txt")
setD(nmax=350,SL.p=3,target.EC=5,plot="FALSE",print.result=FALSE)

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