Man pages for BMILAB/scAPAtrap
Identification and Quantification of Alternative Polyadenylation Sites from Single-cell RNA-seq Data

convertAPAtrapDataConvert the scExpMa list generated by scAPAtrap to other...
countPeaksCalculate the expression level of each peak in each cell with...
dedupByPosDeduplication with umitools
extractBcAndUbExtract barcode and UMI in reads with umi_tools
findPeaksPerform peak calling on a BAM file
findPeaksByStrandPerform peak calling on a BAM file with single strand
findTailsFind the positions with polyA tails genome/chromosome-wide
findTailsByPeaksFind tail positions around/within peak ranges
findUniqueMapFilter a BAM file to preserve unique mappings with samtools
generateAlignBamAlign the sequence to the reference genome with STAR
generateFinalBamGenerate a BAM file for called peaks with featureCounts and...
generateRefIndexBuild the reference genome index with STAR
generateSAFGenerate a peak annotation file
generatescExpMaGenerate single cell expression matrix
initScAPAtrapInitiate scAPAtrap and check/set parameters
loadBpCoveragesLoad a BAM file and calculate coverages of each position with...
reducePeaksReduce peaks number in a countsfile and/or peaksfile
scAPAtrapWrapper function for one-step running of scAPAtrap
separateBamBystrandSeparate BAM files by strand
setToolsSet tool names for running scAPAtrap
setTrapParamsSet the parameters for running scAPAtrap
TRAPFILESTRAPFILES global variable
TRAP.PARAMSGet parameters to run scAPAtrap
BMILAB/scAPAtrap documentation built on Oct. 13, 2023, 2:36 a.m.