bio_binding: Calculate Binding Affinities

In BradyAJohnston/biochemr: Tools For User-Friendly & Reproducible Analysis of Biochemistry Experiments

Calculate Binding Affinities

Description

Calculated binding affinities (Kd) from experimental data that contains dose and response information. Could be titrated concentration and resulting fluorescence, or percentage shift in a EMSA or something similar.

Usage

bio_binding(data, conc, resp, group = NULL, slope = 1)

Arguments

data	data.frame or tibble containing dose response data.
conc	Column with the concentration information.
resp	Column containing the response information.
group	allowed to be free (set slope = NA).
slope	For all 1:1 binding interactions, the slope for the log - logistic curve should be 1. If the interaction has some cooperativity or it is not a 1:1 binding interaction, then this value will change and should be

Value

a tibble

Examples

Puromycin %>%
  bio_binding(conc, rate, group = state)

BradyAJohnston/biochemr documentation built on April 13, 2022, 9:15 p.m.