scrubDoublets: The main function used to identify doublets

Description Usage Arguments Value

Description

The main function used to identify doublets

Usage

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scrubDoublets(exp, n_neighbors = NULL, doublet_score_threshold = NULL,
  sim_doublet_ratio = 2, expected_doublet_rate = 0.06,
  stdev_doublet_rate = 0.02, synthetic_doublet_umi_subsampling = 1,
  use_approx_neighbors = TRUE, distance_metric = "euclidean",
  min_counts = 2, min_cells = 3, min_gene_variability_pctl = 85,
  log_transform = FALSE, z_score = TRUE, n_prin_comps = 30,
  verbose = TRUE)

Arguments

exp

expression matrix of a single-cell RNA-seq data set (gene, cell)

n_neighbors

the number of neighbors used to create the KNN graph

doublet_score_threshold

the threshold used to separate doublets

sim_doublet_ratio

the expected doublet ratio

stdev_doublet_rate

the standard deviation of doublet rate

synthetic_doublet_umi_subsampling

the subsampling rate while simulation

use_approx_neighbors

whether use approx neighbors

distance_metric

which method use measure distance of two nodes

min_counts

minimum read count of cell

min_cells

minimum detected cell of gene

min_gene_variability_pctl

minumum gene variability percentage

log_transform

whether perform log transformation

z_score

whether transfer to z_score

n_prin_comps

the number of prin comps used

verbose

whether display processing message

Value

the doublet label and score for each cell


ChengxiangQiu/rscrublet documentation built on May 3, 2019, 5:23 p.m.