Description Usage Arguments Value
The main function used to identify doublets
1 2 3 4 5 6 7 | scrubDoublets(exp, n_neighbors = NULL, doublet_score_threshold = NULL,
sim_doublet_ratio = 2, expected_doublet_rate = 0.06,
stdev_doublet_rate = 0.02, synthetic_doublet_umi_subsampling = 1,
use_approx_neighbors = TRUE, distance_metric = "euclidean",
min_counts = 2, min_cells = 3, min_gene_variability_pctl = 85,
log_transform = FALSE, z_score = TRUE, n_prin_comps = 30,
verbose = TRUE)
|
exp |
expression matrix of a single-cell RNA-seq data set (gene, cell) |
n_neighbors |
the number of neighbors used to create the KNN graph |
doublet_score_threshold |
the threshold used to separate doublets |
sim_doublet_ratio |
the expected doublet ratio |
stdev_doublet_rate |
the standard deviation of doublet rate |
synthetic_doublet_umi_subsampling |
the subsampling rate while simulation |
use_approx_neighbors |
whether use approx neighbors |
distance_metric |
which method use measure distance of two nodes |
min_counts |
minimum read count of cell |
min_cells |
minimum detected cell of gene |
min_gene_variability_pctl |
minumum gene variability percentage |
log_transform |
whether perform log transformation |
z_score |
whether transfer to z_score |
n_prin_comps |
the number of prin comps used |
verbose |
whether display processing message |
the doublet label and score for each cell
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