package is an interactive interface to explore and visualise
experimental mass spectrometry-based spatial proteomics data. It
relies on the
shiny framework for
interactive visualisation, the
package to handle data and metadata and the
software for spatial proteomics specific data matters. Example spatial
data is available in the
pRoloc suite set of software are distributed as part of the
R/Bioconductor project and are developed
at the Computational Proteomics Unit
Cambridge Centre for Proteomics
labs, at the University of Cambridge.
This document describes the installation of the software, followed by
a basic quick start guide for using
pRolocGUI to search and
visualise the organelle map. Please refer to the respective
documentation and vignettes for full details about the software.
If you use these open-source software for your research, please cite:
Gatto L, Breckels LM, Wieczorek S, Burger T, Lilley KS. Mass-spectrometry-based spatial proteomics data analysis using pRoloc and pRolocdata. Bioinformatics. 2014 May 1;30(9):1322-4. doi:10.1093/bioinformatics/btu013. Epub 2014 Jan 11. PMID:24413670; PMCID:PMC3998135.
Breckels LM, Gatto L, Christoforou A, Groen AJ, Lilley KS, Trotter MW. The effect of organelle discovery upon sub-cellular protein localisation. J Proteomics. 2013 Mar 21. doi:pii: S1874-3919(13)00094-8. 10.1016/j.jprot.2013.02.019. PMID:23523639.
Gatto L., Breckels L.M., Burger T, Nightingale D.J.H., Groen A.J., Campbell C., Mulvey C.M., Christoforou A., Ferro M., Lilley K.S. 'A foundation for reliable spatial proteomics data analysis' Mol Cell Proteomics. 2014 May 20.
pRolocGUI is written in the
programming language. Before installing the software you need to
R and (optionally)
1) Download the latest
R release for your operating system from the
R website and install it.
2) Optional, but
and install the RStudio IDE.
RStudio provides a good code editor and
excellent integration with the
4) Install the Bioconductor packages
R >= 3.1.1 and Bioconductor version
R console, type
if (!requireNamespace("BiocManager", quietly=TRUE)) install.packages("BiocManager") BiocManager::install(c("pRoloc", "pRolocdata", "pRolocGUI"))
The development code on github can also be installed using
install_github). New pre-release features
might not be documented or thoroughly tested and could substantially
change prior to release. Use at your own risks.
Before using a package's functionality, it needs to be loaded:
We first load data from
Christoforou et al 2016
distributed in the
There are 3 different visualisation applications currently
These apps are launched using the
pRolocVis function and
object, which is an
MSnSet containing the data
one wishes to interrogate. One may also specify which app
they wish to use by using the
app argument, see
for more details. The default app that is loaded if
app is not specified is the
The graphical interface is described in details in the package
vignette that is included in the package itself (get it by typing
R), available by clicking the
the interface is loaded or can be
pRolocGUI- This tutorial is for the older legacy applications. New videos will appear shortly for the new applications.
pRolocGUIto explore and visualise experimental spatial proteomics data - This tutorial is for the older legacy applications. New videos will appear shortly for the new applications.
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