R/data.R

#' Activation
#'
#' An experiment performed to assess the in vitro activation of OT-I and OT-II transgenic T cells co-cultured with
#' OVA-presenting dendritic cells (DCs). This data set is comprised of two samples saved as a \code{flowSet} object.
#' 
#' Pooled DCs & T cells were labelled as follows:
#' - Hoechst 33258, a DNA interchelating dye which fluoresces in Alexa Fluor 405-A and Alexa Fluor 430-A channels
#' - CD11c-APC-Cy7 to identify DCs within the samples.
#' - Va2-PE to identify cells which express the OVA-specific TCR (i.e. OT-I and OT-II T cells).
#' - CD8-FITC to identify the CD8+ OT-I transgenic T cells (Alexa Fluor 488-A).
#' - CD4-Alexa Fluor 700 to identify the CD4+ OT-II transgenic T cells (Alexa Fluor 700-A)
#' - CD44-APC to assess T cell activation (Alexa Fluor 647-A)
#' - CD69-PerCP Cy5.5 to assess T cell activation (7-AAD-A)
#' 
#' @format A flowSet containing two flowFrame objects:
#'
"Activation"
DillonHammill/CytoGate documentation built on May 16, 2019, 7:20 a.m.