README.md
In DmitryMarkovich/Thrombin_Analyzer: Thrombin Analyzer

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This application performs data analysis in thrombin generation experiments. Below is the step-by-step guide that explains how to use it.

Calibration

Calibration signal tab visualizes the results of analysis of a calibration signal. Use the Load calibration data file button and choose a suitable signal to load. Then, choose a model to fit the loaded signal using the Select model to fit calibration signal. Parameters obtained from the model fitted to the signal will be shown in Parameters tab.

Thrombin generation

Thrombin generation signal tab visualizes the results of analysis of a thrombin generation signal. Use the Load thrombin generation data file button and choose a suitable signal to load. Then, choose a model to fit the loaded signal using the Select model to fit thrombin generation signal. Use Thrombogram tab to visualize the thrombogram and the thrombin velocity of the signal. Parameters obtained from the model fitted to the signal will be shown in Parameters tab.

Parameters

Analyze a calibration or a thrombin generation signal or both following the steps above and check the parameters with the Parameters tab.

Controls

SC time, min the minimal length of the measurement that will cause the app to try out models with substrate consumption (SC). If You want all the signals in Your dataset to use models without SC, set it to a value larger than the maximal time in the dataset. The default value is 121 --- all signals that are shorter than 121 minutes will not be fitted with SC models by the Auto model.
SC ratio this ratio is used by Auto model to make a decision whether SC model should be preferred to the non-SC model. If the residual standard error of the fit without SC is greater than SC ratio multiplied by the residual standard error of the fit with SC, than SC model would not be preferred. It means that if a fit of an SC model does not increase the residual standard error of the fit enough, there is no need for SC model for this signal. The default value is 1.7.
Mode --- this radio button switches the mode of the app from Speed to Accuracy. In Speed mode, no attempts to fit more complex models will be made to save time, whereas in Accuracy mode the app will persistently try to fit more complicated models to the data for better approximation. The default mode is Speed.

Dataset

Dataset tab visualizes the results of the analysis of a dataset from a thrombin generation experiment. Use the Load dataset file to load a dataset, and press Analyze! to analyze it. Overlay thrombograms and check the details of the performed analysis using the Overlay and Overlay details tabs.

If You have the results and the parameters available for the dataset, You can alternatively load them using the Load parameters and Load results.

Demo signals

In case there are no data on Your computer, download some of the provided demo signals by choosing from options in Select a demo signal to try out models on data!. The data You choose is visualized in Demo signals tab. Download data, and analyze it following the steps above.