process_seurat: Process Seurat objects

Description Usage Arguments Details Value

View source: R/process_seurat.R

Description

This command creates a Seurat object, filters for doublets and dead cells runs UMap, Tsne, and creates a clusterplot

Usage

1
process_seurat(dirlist, outlist)

Arguments

dirlist

takes a list of 10x sequencing directories to process

outlist

takes a list of what you would like the output objects to be called

Details

you may need to modify the criteria for subsetting. If your elbow plot is far from 18 you will need to change it most likely. process_seurat()

Value

a list of Seurat objects that in which doublets and dead cells have been filtered variable features have been identified, normalized by SCtransform,


H-E-A-L-T-H/UsefulFunctions documentation built on Oct. 30, 2019, 6:37 p.m.