Description Usage Arguments Details Value
View source: R/process_seurat.R
This command creates a Seurat object, filters for doublets and dead cells runs UMap, Tsne, and creates a clusterplot
1 | process_seurat(dirlist, outlist)
|
dirlist |
takes a list of 10x sequencing directories to process |
outlist |
takes a list of what you would like the output objects to be called |
you may need to modify the criteria for subsetting. If your elbow plot is far from 18 you will need to change it most likely. process_seurat()
a list of Seurat objects that in which doublets and dead cells have been filtered variable features have been identified, normalized by SCtransform,
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