formatRawFluorescenceFile: Format raw qPCR fluorescence file data for results_Table or...

In HannerLab/MDMAPR: Molecular Detection Mapping and Analysis Platform

Description

Function formats raw qPCR fluoresence file data from MIC, StepOnePlus or Biomeme two3/Franklin machines into table that includes well location names and fluorescence data for each reaction cycle. The table is written to the local machine directory as a CSV file. The formatted data can be copied and pasted into the results_Table or standardCurveResults_Table, which are used in the MDMAPR 2.0 MySQL database.

Usage

formatRawFluorescenceFile(rawFluorescenceFile, platform, outputFileName)

Arguments

rawFluorescenceFile	defines raw qPCR fluorescence file to read in.
platform	defines platform fluorescence file was generated from.
outputFileName	defines output name for CSV file that data will be written to.

Value

Writes CSV file to local directory.

Examples

## Not run: 
formatRawFluorescenceFile("rawFluorescenceFile.xlsx",
                          "StepOnePlus",
                          "formatted_SOP_fluorescence.csv")

formatRawFluorescenceFile("rawFluorescenceFile.csv",
                          "MIC",
                          "formatted_MIC_fluorescence.csv")

formatRawFluorescenceFile("rawFluorescenceFile.csv",
                          "Biomeme two3/Franklin",
                          "formatted_Biomeme_fluorescence.csv")

## End(Not run)

HannerLab/MDMAPR documentation built on Dec. 17, 2021, 10:28 p.m.