tidy_lab: Tidy a lab object

In KaiAragaki/ragaki: Reduce Annoyances

Description

Tidy a lab object

Tidy a nanodrop object

Usage

tidy_lab(x, ...)

## S3 method for class 'nanodrop'
tidy_lab(x, force_tidy = FALSE, ...)

Arguments

x	a nanodrop object
...	Arguments passed to their respective methods
force_tidy	Logical. Should the tidying take place, even if the is_tidy attribute is TRUE?

Details

This function:

• renames columns to sensible substitutes

• converts date-times to ISO 8601-esque date-time format (YYYY-MM-DD HH:MM:SS vs YYYY-MM-DDTHH:MM:SSZ)

It's recommended that you do any manipulations to these data after you tidy, rather than before, as tidy_nanodrop expects the output to be fairly similar to the output from read_nanodrop.

A tidy nanodrop object will (usually) contain the following columns:

date

The date-time of sample reading, as YYYY-MM-DD HH:MM:SS

sample_name

The name of the sample provided by the NanoDrop itself

conc

The concentration of nucleic acid in ng/uL

a260_280

The absorbance at 260nm / absorbance at 280nm. Typically a marker of protein contamination. Pure nucleic acid is typically around 2.

a260_230

The absorbance at 260nm / absorbance at 230nm. Typically a marker of guanidine salt contamination. Pure nucleic acid is typically around 2.

a260

The absorbance at 260nm, the wavelength nucleic acids absorb most strongly.

a280

The absorbance at 280nm, the wavelength proteins absorb most strongly.

tube_name

The label given the physical tube that contains sample

cell_line

The cell line from which the sample came, if applicable

experimental_condition

A brief description of the conditions these samples were subject to for the experiment

tube_name, cell_line, and experimental_condition are always unfilled, because these data are not within nanodrop data. However, they are provided because they are included in the masterlist data, and it's wise to fill them out for your records.

The remaining columns are typically unused.

Value

a nanodrop object

KaiAragaki/ragaki documentation built on Dec. 25, 2021, 2:24 a.m.