getIntensity: To get the final extracted intensity of control line and test...

In NPhogat/GNSplex: An R package for analysis of the data of gold nanoparticles based singleplex bioassay

Description

Function getIntensity reads the .jpeg files of the lateral flow assay and returns the extracted intensity of the control line and test line signals

Usage

getIntensity(imagefile, cut1 = 8, cut2 = 25, pi = 1.2, fp = TRUE)

Arguments

imagefile	.jpeg image of the lateral flow assay
cut1	first value on X-axis (index) to cut the control line or test line peak, as obtained from the function readIGNScutprocess
cut2	second value on x-axis (index) to cut the control line or test line peak as obtained from the readIGNScutprocess
pi	the value of intensity to be applied as baseline correction for control line or test line peaks (signals). These should be the same values, as applied in the readIGNScutprocess
fp	TRUE or FALSE. When TRUE, one can implement for first peak control line. When FALSE; one can implement for second peak test line.

Details

Allows the user to get the baseline corrected values of intensities of control line or test line

Value

baseline corrected intensities of control line and test line

Author(s)

Navneet Phogat, Matthias Kohl, [e-mail]

Examples

#'## to read and see complete plot of signal
file3 <- system.file("exData", "Serum30nM_2.JPG", package = "GNSplex")
readIGNSplot(file3)
## to read and cut the first peak (control line signal) and to do baseline
##correction
file3 <- system.file("exData", "Serum30nM_2.JPG", package = "GNSplex")
readIGNScutprocess(imagefile = file3, fp = TRUE, cut1 = 3, cut2 = 13, pi = 2.0)
## To cut the second peak (test line signal) and to do baseline correction
readIGNScutprocess(imagefile = file3, fp = FALSE, cut1 = 17, cut2 = 30, pi = 2.0)
## To get the baseline corrected intensity of control line (peak 1)
getIntensity(imagefile = file3, cut1 = 3, cut2 = 13, pi = 2.0)
## to get the baseline corrected intensity of test line (peak 2)
getIntensity(imagefile = file3, cut1 = 17, cut2 = 30, pi = 2.0, fp = FALSE)

NPhogat/GNSplex documentation built on May 8, 2020, 9:28 p.m.