Description Usage Format Details References Examples
Time-course RNA-seq leaf and root data from "Transcriptomic analysis of field-droughted sorghum from seedling to maturity reveals biotic and metabolic responses"
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A list with two related datasets: a gene expression data set (element
data
) and corresponding meta data (element meta
). The column
names of data
correspond to the rownames of meta
, linking the
samples together in the two datasets. See examples for accessing these two
data.frames.
This are two datasets from a mRNA-Seq time-course experiment,
measuring gene expression across time in the plant sorghum. One dataset
corresponds to the leaf of the plant (varoquaux2019leaf
), the other
to the root from the same sets of plants (varoquaux2019leaf
). Each
sample sequenced is actually a pool of at least 10 plants. Note that leaf
and root samples were processed and sequenced as two separate batches.
Any comparison between the two datasets should be traded with care.
data
is a data frame with 34,211 rows corresponding to
sorghum genes with
(Phytozome
v3.0 gene ids). Root has 198 columns and leaf has 197 columns,
corresponding to samples. The root and leaf samples are from the same
plants, and have ids in the form of "0622162L05" or "0622162R05", with "L"
corresponding to the leaf and "R" corresponding to the root sample. Some
samples were removed for quality control reasons, resulting in slightly
different numbers of samples between the leaf and root.
The data provided is the raw data. Note that leafs and roots were sequenced as two different batches.
meta
is a data frame with rows corresponding to samples and
and columns with meta data. These columns are the same across leaf and
root, allowing for easy reuse of code across the datasets:
Barcode of the sample, same as row name
Internal Barcode given to the sample by the sequencing facility (JGI)
Plot from which the sample was taken
Numeric value corresponding to the week sample was taken
Each condition combination was replicated three times, but not all replicates were sequenced. Each replicate came from a different plot. The replicate id tracks this plot for each condition. However, replicate 1 in one condition is a completely different plot from replicate 1 in another condition, though the replicates were physically close to each other in the field.
Genotype (i.e. variety) of the sample, one of BT642 or RT430
The drought condition, one of "Control" (regular watering), "Postflowering" (drought applied post flowering of the plant), or "Preflowering" (drought applied pre-flowering of the plant, and then watering resumed after flowering)
Either "Leaves" or "Root", only relevant if merging the two datasets
The day sample collected, in form of "Day14" (corresponding to Week 2)
Row plant was sampled from within the block
logical, indicating whether the week sampled corresponding to the plant flowering
The date sample was collected
The time of day plant was collected
The number of plants pooled for the sample
The Julian date of flowering for the block the sample was collected from
The date of flowering for the block the sample was collected from
The week of flowering for the block the sample was collected from
The day of flowering for the block the sample was collected from
Identifies timepoints with no replicates
Full set of factors (Genotype x Treatment x Week)
The following variables are features measured at the end of planting when the remaining plants from the plot were harvested:
The following variables are features regarding the quality of the mapping of the sequences from the sample:
Varoquaux et al, 2019 (PNAS)
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dataLeaf = attr(varoquaux2019leaf, "data")
metaLeaf = attr(varoquaux2019leaf, "meta")
data(varoquaux2019root)
dataRoot = attr(varoquaux2019root, "data")
metaRoot = attr(varoquaux2019root, "meta")
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