Description Usage Arguments Value
design a combinatorial library
1 2 3 4 5 6 7 8 9 10 11 12 13 | design_combo_lib(
design_file,
all_by_all_gene = F,
row_genes = NULL,
col_genes = NULL,
ref_genes = NULL,
gene_pairs = NULL,
guide_pairing = "all",
dual_orientation = F,
gene_col = "Target Gene Symbol",
guide_col = "sgRNA Sequence",
guide_rank = "Pick Order"
)
|
design_file |
from GPP's CRISPick |
all_by_all_gene |
design the simplest combinatorial library, pairing all genes in the design file with all other genes |
row_genes |
genes to be paired with col_genes |
col_genes |
genes to be paired with row_genes |
ref_genes |
all genes get paired with these |
gene_pairs |
programmed gene pairs |
guide_pairing |
either 'all' or 'rank' |
dual_orientation |
pair guides in both directions |
gene_col |
column name with genes from CRISPick |
guide_col |
column name with guides from CRISPick |
guide_rank |
column name with guide ranks from CRISPick |
combinatorial designs with columns gene_x, gene_y, guide_x, guide_y
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