design_combo_lib: design a combinatorial library

In PeterDeWeirdt/combibuild: What the Package Does (One Line, Title Case)

Description

design a combinatorial library

Usage

design_combo_lib(
  design_file,
  all_by_all_gene = F,
  row_genes = NULL,
  col_genes = NULL,
  ref_genes = NULL,
  gene_pairs = NULL,
  guide_pairing = "all",
  dual_orientation = F,
  gene_col = "Target Gene Symbol",
  guide_col = "sgRNA Sequence",
  guide_rank = "Pick Order"
)

Arguments

design_file	from GPP's CRISPick
all_by_all_gene	design the simplest combinatorial library, pairing all genes in the design file with all other genes
row_genes	genes to be paired with col_genes
col_genes	genes to be paired with row_genes
ref_genes	all genes get paired with these
gene_pairs	programmed gene pairs
guide_pairing	either 'all' or 'rank'
dual_orientation	pair guides in both directions
gene_col	column name with genes from CRISPick
guide_col	column name with guides from CRISPick
guide_rank	column name with guide ranks from CRISPick

Value

combinatorial designs with columns gene_x, gene_y, guide_x, guide_y

PeterDeWeirdt/combibuild documentation built on March 18, 2020, 7:36 a.m.