positiveFactor: Acessor functions for the 'positiveFactor' slot in a...
In Shedimus/NanoStringDiff: Differential Expression Analysis of NanoString nCounter Data
Description
Usage
Arguments
Details
Value
Author(s)
See Also
Examples
Description
Positive size factors can be used to adjust all platform associated
sources of variation.
Usage
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## S4 method for signature 'NanoStringSet'
positiveFactor(object)
## S4 replacement method for signature 'NanoStringSet,numeric'
positiveFactor(object) <- value
Arguments
object
A NanoStringSet object.
value
A vector of positive size factors.
Details
The observed counts including negative control genes and housekeeping control
genes might be effect by some experimental factors like hybridization and
binding efficiency. In order to get the true rate of gene expression,
these variations must be normalized. Positive size factors can normalize
this kind of variation.
Value
A vector contain positive size factors
Author(s)
Hong Wang <hong.wang@uky.edu>
chi wang <chi.wang@uky.edu>
See Also
positiveControl
Examples
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data(NanoStringData)
## obtain positive factors
positiveFactor(NanoStringData)
## assign a vector
n=ncol(exprs(NanoStringData))
positiveFactor(NanoStringData)=rep(1,n)
Shedimus/NanoStringDiff documentation built on Dec. 5, 2019, 1:56 a.m.
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Description Usage Arguments Details Value Author(s) See Also Examples
Description
Positive size factors can be used to adjust all platform associated sources of variation.
Usage
1 2 3 4 | ## S4 method for signature 'NanoStringSet'
positiveFactor(object)
## S4 replacement method for signature 'NanoStringSet,numeric'
positiveFactor(object) <- value
|
Arguments
object |
A NanoStringSet object. |
value |
A vector of positive size factors. |
Details
The observed counts including negative control genes and housekeeping control genes might be effect by some experimental factors like hybridization and binding efficiency. In order to get the true rate of gene expression, these variations must be normalized. Positive size factors can normalize this kind of variation.
Value
A vector contain positive size factors
Author(s)
Hong Wang <hong.wang@uky.edu> chi wang <chi.wang@uky.edu>
See Also
positiveControl
Examples
1 2 3 4 5 6 7 | data(NanoStringData)
## obtain positive factors
positiveFactor(NanoStringData)
## assign a vector
n=ncol(exprs(NanoStringData))
positiveFactor(NanoStringData)=rep(1,n)
|
R Package Documentation
Browse R Packages
We want your feedback!
Note that we can't provide technical support on individual packages. You should contact the package authors for that.
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