positiveFactor: Acessor functions for the 'positiveFactor' slot in a...

Description Usage Arguments Details Value Author(s) See Also Examples

Description

Positive size factors can be used to adjust all platform associated sources of variation.

Usage

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## S4 method for signature 'NanoStringSet'
positiveFactor(object)
## S4 replacement method for signature 'NanoStringSet,numeric'
positiveFactor(object) <- value

Arguments

object

A NanoStringSet object.

value

A vector of positive size factors.

Details

The observed counts including negative control genes and housekeeping control genes might be effect by some experimental factors like hybridization and binding efficiency. In order to get the true rate of gene expression, these variations must be normalized. Positive size factors can normalize this kind of variation.

Value

A vector contain positive size factors

Author(s)

Hong Wang <hong.wang@uky.edu> chi wang <chi.wang@uky.edu>

See Also

positiveControl

Examples

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data(NanoStringData)
## obtain positive factors
positiveFactor(NanoStringData)

## assign a vector
n=ncol(exprs(NanoStringData))
positiveFactor(NanoStringData)=rep(1,n)

Shedimus/NanoStringDiff documentation built on Dec. 5, 2019, 1:56 a.m.