CalculateAmounts: CalculateAmounts

In TomHarrop/qubitr: Analyse Results from the Clariostar Qubit Assay

Description

Use the background_subtracted fluorescence values to make a lm from the standard_wells and predict the amounts in the other wells.

Usage

CalculateAmounts(background_subtracted, standard_wells)

Arguments

background_subtracted	A data.table of background-subtracted Clariostar readings. Must have a column called background_subtracted.
standard_wells	A data.table of standards with the columns well and amount, e.g. data.table(well = paste0(LETTERS[3:8], 5), amount = c(rep(10, 3), rep(100, 3))) well amount 1: C5 10 2: D5 10 3: E5 10 4: F5 100 5: G5 100 6: H5 100

Value

Returns a data.table of with an additional column called calculated_amount containing the result of predict on the background_subtracted values using the lm from the standards

TomHarrop/qubitr documentation built on May 14, 2019, 9:38 a.m.