spikeindata: Example of normalized datasets from raw_data,

Description Usage Format Details Examples

Description

We normalize the intensity of the light peptides using that of the heavy peptides. This corrects any systematic errors that can occur during a run or across replicates. The calculation is greatly simplified by the use of the tidyr and dplyr packages. The area from all the different peptide fragments is first summed then log transformed. The median intensity of the reference heavy peptides medianlog2heavy is calculated. Their intensities should ideally remain constant across runs since the spiked concentration of the heavy peptide is constant. The difference between the median for all the heavy peptide spikes is calculated. It is then used to correct (i.e. to normalize) the intensity of the light peptides log2light to obtain the adjusted intensity log2light_norm. The intensity is finally converted back to original space. Details are available in vignette. The variables are as follows:

Usage

1

Format

A data frame with 30 rows and 4 variables.

Details

Examples

1

Vitek-Lab/MSstatsLOBD documentation built on Dec. 18, 2021, 6:20 p.m.