get.homology.template: Create a donor oligo based on input sequence and sgRNA guides

Description Usage Arguments Value

View source: R/templateDesigner.R

Description

This function finds 60bp homology arms up and downstream of the sgRNA cut site and merges the left homology arm, insert and right homology arm to build a donor template

Usage

1
get.homology.template(pam.res, full.seq, ins)

Arguments

pam.res

The dataframe results after running find.pams()

full.seq

A sequence 150bp or longer

ins

A DNA sequence that acts as the insert

Value

a dataframe with the guide sequence and the corresponding donor oligo


ahmcewan/templateDesigner documentation built on Oct. 12, 2020, 12:04 a.m.