calculate_rh: Calculate hydrodynamic radii

View source: R/calculate_rh.R

calculate_rhR Documentation

Calculate hydrodynamic radii

Description

Calculate hydrodynamic radii

Usage

calculate_rh(
  t1,
  focus = 10,
  transition = 1,
  w = 500/1e+06,
  Vc = 0.0025,
  Vout = 0.001,
  Vin = 5e-04,
  temp = 25,
  eta = 0.00089,
  dims = chamber_dims
)

Arguments

t1

Retention time, in minutes.

focus

Focus period, in minutes.

transition

Transition time between focusing and elution, in minutes.

w

Channel thickness, in metres.

Vc

Cross-flow rate, in L/min.

Vout

Detector flow rate, in L/min.

Vin

Injection flow rate, in L/min.

temp

Temperature, degrees Celsius.

eta

Dynamic viscosity, in N * s / m^2.

dims

A list with the elements L, b1, b2, and z1, as defined in Wang et al. (2018, units of cm).

Value

A vector of hydrodynamic radii in metres.

References

  1. Wang, J.-L.; Alasonati, E.; Fisicaro, P.; Benedetti, M. F.; Martin, M. Theoretical and Experimental Investigation of the Focusing Position in Asymmetrical Flow Field-Flow Fractionation (AF4). Journal of Chromatography A 2018, 1561, 67–75. https://doi.org/10.1016/j.chroma.2018.04.056.

  2. Kumar, A. A Simple Correlation for Estimating Viscosities of Solutions of Salts in Aqueous, Non-Aqueous and Mixed Solvents Applicable to High Concentration, Temperature and Pressure. Can. J. Chem. Eng. 1993, 71 (6), 948–954. https://doi.org/10.1002/cjce.5450710617.

  3. Messaud, F. A.; Sanderson, R. D.; Runyon, J. R.; Otte, T.; Pasch, H.; Williams, S. K. R. An Overview on Field-Flow Fractionation Techniques and Their Applications in the Separation and Characterization of Polymers. Progress in Polymer Science 2009, 34 (4), 351–368. https://doi.org/10.1016/j.progpolymsci.2008.11.001.

  4. Wahlund, K. Gustav.; Giddings, J. Calvin. Properties of an Asymmetrical Flow Field-Flow Fractionation Channel Having One Permeable Wall. Anal. Chem. 1987, 59 (9), 1332–1339. https://doi.org/10.1021/ac00136a016.

  5. Tanase, M.; Zolla, V.; Clement, C. C.; Borghi, F.; Urbanska, A. M.; Rodriguez-Navarro, J. A.; Roda, B.; Zattoni, A.; Reschiglian, P.; Cuervo, A. M.; Santambrogio, L. Hydrodynamic Size-Based Separation and Characterization of Protein Aggregates from Total Cell Lysates. Nat Protoc 2015, 10 (1), 134–148. https://doi.org/10.1038/nprot.2015.009.

  6. Field-Flow Fractionation in Biopolymer Analysis; Williams, S. K. R., Caldwell, K. D., Eds.; Springer Vienna: Vienna, 2012. https://doi.org/10.1007/978-3-7091-0154-4.

Examples

tr <- seq(11.6, 15.6, by = .1)
calculate_rh(tr)

bentrueman/fffprocessr documentation built on June 23, 2024, 1:23 a.m.