create_target_BEDfile: Create a New Entry on the Master Bedfile

In blaserlab/genotyping.functions: Supplies Functios For Zebrafish Genotyping

Create a New Entry on the Master Bedfile

Description

Use this function to find a crispr target sequence and write to the master bedfile. Query should be a 23-letter string corresponding to the gRNA used to make the cripsr, entered 5' to 3'. The target recognition site should first, followed by the PAM. By convention, all crispr target names will be converted to upper case. If either the target range or name are duplicated, an error will result.

Usage

create_target_BEDfile(
  query,
  crispr_target_name,
  genome = c("Drerio", "GFP"),
  master_bed = "data-raw/master.bed"
)

Arguments

query	the guide RNA sequence, using DNA nucleotides
crispr_target_name	The name for the crispr site.

Value

Nothing. Adds an entry to the master bedfile.

See Also

character(0) DNAString-class,matchPattern BSgenome.Drerio.blaserlabgenotyping.dr11

blaserlab/genotyping.functions documentation built on Oct. 16, 2024, 1:40 a.m.