find_proto: find_proto

Description Usage Arguments Value

View source: R/protospacers.R

Description

Function to identify all candidate guide (protospacer) sequences from a given genomic region.

Usage

1
find_proto(d_seq = "TGATCTACTAGAGACTACTAACGGGGATACATAG", l = 20, PAM = "NGG")

Arguments

d_seq

input DNA sequence for genomic region from which to identify protospacer sequences (in capital letters).

l

protospacer length.

PAM

sequence to match.

Value

a dataframe with columns.

  1. start.

  2. end.

  3. protospacer sequence.

  4. PAM sequence (e.g. CGG as the sequence that matched the PAM sequence NGG).

  5. Strand (+ or -).


c1au6i0/crispR documentation built on Feb. 27, 2020, 12:42 a.m.