Description Usage Arguments Value
Function to identify all candidate guide (protospacer) sequences from a given genomic region.
1 | find_proto(d_seq = "TGATCTACTAGAGACTACTAACGGGGATACATAG", l = 20, PAM = "NGG")
|
d_seq |
input DNA sequence for genomic region from which to identify protospacer sequences (in capital letters). |
l |
protospacer length. |
PAM |
sequence to match. |
a dataframe with columns.
start.
end.
protospacer sequence.
PAM sequence (e.g. CGG as the sequence that matched the PAM sequence NGG).
Strand (+ or -).
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