In changebio/MAGPA: Multivariate Analysis of Genotype–Phenotype Association and Visualization for 3D Image

source("setup.R")
knitr::opts_chunk$set(rgl.newwindow = TRUE)
set.seed(123)

Introduction

The MAGPA (multivariate analysis of genotype–phenotype association) is a package of multivariate correlation analysis and an interactive visualization tool for 3D image. This package was implemented for genetic association analysis of facial phenotypes and visualization related results. In addition, it can also be used in genome-wide association analysis of other multivariate phenotypes, especially three-dimensional image data. It can not only receive the prepared features, but also preprocess the features with principal component analysis and automatically select the number of variables. The genotype should be a SnpMatrix, which is a special object holding large arrays of single nucleotide polymorphism (SNP). Then，canonical correlation analysis (CCA) is used to extract the linear combination of variables to maximize the correlation with each SNP. For the interactive visualization, the function visual3d is required to provide at least a reference of 3D image object and a vector, such as the phenotypic changes under different genotypes, which is highly customized. it can draw a 3D object with different style and gradient colors.

Installation

The MAGPA package can be installed via GitHub. Befor installation, R or Rstudio should be installed on computer. To install the latest version of MAGPA through GitHub. Please run the following command in R.

if (!require("devtools"))
  install.packages("devtools")
devtools::install_github("changebio/MAGPA")

library(MAGPA)

Multivariate Analysis of Genotype–Phenotype Association

About the Data

In this vignette we will look at a toy example, which includes genotype data in geno and phenotype data in pheno. the genotype data and phenotype data are used to demonstrate how to use the function magpa and show the one of the possible input to the function. The variable geno is a list with a SnpMatrix genotypes (2000 rows, 50 columns) and a data frame map. The variable pheno is a matrix (2000 rows, 300 columns) expanded by 2000 samples with 100 three-dimensional coordinate in each sample.

data(geno)
str(geno)

data(pheno)
str(pheno)

How to use magpa

If your phenotype is pre-prepared, you can follow the step 2.2. Otherwise, you can follow the step 1, which set pca argument to TRUE. Or you can follow the step 2.1 to select features, and then follow the step 2.2.

processing phenotype and multivariate association

the function magpa is performed multivariate analysis of genotype–phenotype association based on CCA, which calls cca in the yacca package to do canonical correlation analysis. The first argument of magpa can be a file prefix name of plink output (.bed, .bim, .fam), an object read by read.plink of the snpStats package, or a SnpMatrix. The second argument is the phenotypic matrix (rows are the number of samples, and columns are the number of features).

gpa <- magpa(geno,pheno,pca = TRUE)
head(gpa)

automatic extraction of the principal components

The function pcapheno is implemented to automatically extract the principal components from high-dimension data, which calls paran to performs Horn's parallel analysis for evaluating the components retained in a principle component analysis.

paral<- pcapheno(pheno)
new_pheno<- paral$pheno
head(new_pheno)

multivariate association with prepared phenotype

gpa<- magpa(geno,new_pheno)
head(gpa)

The result in a matrix included SNP, CHR, position, MAF, canonical correlation, chisq, and pvalue. It can be used to draw QQ plot and Manhattan plot.

Visualization for 3D Face

The function visual3d is an interactive graphing function based on the rgl package. the function is very flexible. The first argument is a reference of 3D image object, whose file (with .obj suffix) can be read by the readobj function. the second argument can be a vector or list. the thrid argument is a vector of colors or a color palette, if it is not given, the default color palette will be used. Two examples of using visual3d are as follows.You can see more in the help documentation.

example 1 : facial segmentation

Here, refface includes two object, a 3D reference face ref.m and a label vecter of facial segmentation seg.idx. The human face was divided into 10 segments, as shown in the figure.

knitr::include_graphics("segments.png")

ref.m contains 32251 vertices, each of which is labeled in one of 10 categories. the information is in seg.idx.

data(refface)
ref.m <- refface$ref.m
seg.idx<- refface$seg.idx
visual3d(ref.m,seg.idx,col = colorbar(1:10,c("blue", "cyan","green", "yellow", "orange","red")))
rglwidget()

example 2 : heatmap of nose region

The nose region was extracted from face by Meshexract function. Then, the volume of each tetrahedron made by tri-mesh and origin is calculated by Meshvolume function. visual3d shows the heatmap of the nose region.

clear3d() # Remove the earlier display
nose.idx<- which(seg.idx==10)
nose.m <- Meshextract(ref.m,nose.idx)
nose.vol<- Meshvolume(nose.m)
visual3d(nose.m,nose.vol)
rglwidget(reuse = FALSE)

Sessioninfo

sessionInfo()

changebio/MAGPA documentation built on June 15, 2020, 9:26 p.m.